RIA1/YNL163C Protein Information

Standard Name	Ria1p
Systematic Name	Ynl163cp
Alias	Efl1p
ORF Classification	Verified
Description	Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes (1, 2, 3, 4, 5, 6)
Name Description	RIbosome Assembly 1

Experimental Data
Molecules/cell	7770 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,110
Molecular Weight (Da)	124,462
Isoelectric Point (pI)	4.86

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ria1p (InterPro)
Physical Interactions	There are 8 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013BB94 \| P53893 MIPS: YNL163C NCBI: 1050790 \| 1302132 \| 1730796 \| 6324166 \| NP_014236.1 \| NM_001183001.1 GenBank/EMBL/DDBJ: DAA10385.1 \| X92517 \| Z71439
External Classifications	EC: 3.6.5.- [Hydrolases, Acting on GTP; involved in cellular and subcellular movement]

Amino Acid Sequence (or in FASTA format)
1 MPRVESETYK RLQNDPSCIR NICIVAHVDH GKTSLSDSLL ASNGIISQRL 51 AGKIRFLDAR PDEQLRGITM ESSAISLYFR VLRKQEGSDE PLVSEHLVNL 101 IDSPGHIDFS SEVSAASRLC DGAVVLVDVV EGVCSQTVTV LRQCWTEKLK 151 PILVLNKIDR LITELQLTPQ EAYIHLSKVI EQVNSVIGSF FANERQLDDL 201 FWREQLEKNE NAEYIEKDDS GIYFNPTDNN VIFASAIDGW GFNIGQLAKF 251 YEQKLGAKRE NLQKVLWGDF YMDPKTKKII NNKGLKGRSL KPLFTSLILE 301 NIWKIYQNII TSRDSEMVEK IAKTLNIKLL ARDLRSKDDK QLLRTIMGQW 351 LPVSTAVLLT VIEKLPSPLE SQTDRLNTIL VSESDTAAMD PRLLKAMKTC 401 DKEGPVSAYV SKMLSIPREE LPVESKRIAS SDELMERSRK AREEALNAAK 451 HAGMVENMAM MDLNDNSKNT SDLYKRAKDT VMTPEVGEQT KPKPSRNNDV 501 FCVVSEPSSA LDLEFEYEGE DDSDSQDNFG LDFVPTDIDP NDPLSSMFEY 551 EEEDPLLESI KQISEDVNDE VDDIFDEKEE CLVAFARIYS GTLRVGQEIS 601 VLGPKYDPKC PEEHIETAII THLYLFMGKE LVPLDVCPSG NIVGIRGLAG 651 KVLKSGTLIE KGVQGVNLAG VNFHFTPIVR VAVEPANPVE MSKLVRGLKL 701 LDQADPCVHT YVENTGEHIL CTAGELHLER CLKDLTERFA GIEITHSEPA 751 IPYRETFLSA SDMNPPQNSQ LGRGVHELLL SQYKITFRTF PLSGKVTDFL 801 SQHQNSIKNI LKTSTSSMDP VIESTGSSFL DKKSLLVAFE EVINQEEKSR 851 ELLSGFKVKL AGFGPSRVGC NILLSQDNLL GSLFEGTPAA FEYSDSIKNG 901 FQLAVSEGPL ANEPVQGMCV LVESVHKMSQ DEIESIEDPR YQQHIVDLSG 951 RLITSTRDAI HEAFLDWSPR IMWAIYSCDI QTSVDVLGKV YAVILQRHGK 1001 IISEEMKEGT PFFQIEAHVP VVEAFGLSED IRKRTSGAAQ PQLVFSGFEC 1051 IDLDPFWVPT TEEELEELGD TADRENIARK HMNAIRRRKG LFIEEKVVEN 1101 AEKQRTLKKN *

Amino Acid Sequence (or in FASTA format)

       1  MPRVESETYK RLQNDPSCIR NICIVAHVDH GKTSLSDSLL ASNGIISQRL
      51  AGKIRFLDAR PDEQLRGITM ESSAISLYFR VLRKQEGSDE PLVSEHLVNL
     101  IDSPGHIDFS SEVSAASRLC DGAVVLVDVV EGVCSQTVTV LRQCWTEKLK
     151  PILVLNKIDR LITELQLTPQ EAYIHLSKVI EQVNSVIGSF FANERQLDDL
     201  FWREQLEKNE NAEYIEKDDS GIYFNPTDNN VIFASAIDGW GFNIGQLAKF
     251  YEQKLGAKRE NLQKVLWGDF YMDPKTKKII NNKGLKGRSL KPLFTSLILE
     301  NIWKIYQNII TSRDSEMVEK IAKTLNIKLL ARDLRSKDDK QLLRTIMGQW
     351  LPVSTAVLLT VIEKLPSPLE SQTDRLNTIL VSESDTAAMD PRLLKAMKTC
     401  DKEGPVSAYV SKMLSIPREE LPVESKRIAS SDELMERSRK AREEALNAAK
     451  HAGMVENMAM MDLNDNSKNT SDLYKRAKDT VMTPEVGEQT KPKPSRNNDV
     501  FCVVSEPSSA LDLEFEYEGE DDSDSQDNFG LDFVPTDIDP NDPLSSMFEY
     551  EEEDPLLESI KQISEDVNDE VDDIFDEKEE CLVAFARIYS GTLRVGQEIS
     601  VLGPKYDPKC PEEHIETAII THLYLFMGKE LVPLDVCPSG NIVGIRGLAG
     651  KVLKSGTLIE KGVQGVNLAG VNFHFTPIVR VAVEPANPVE MSKLVRGLKL
     701  LDQADPCVHT YVENTGEHIL CTAGELHLER CLKDLTERFA GIEITHSEPA
     751  IPYRETFLSA SDMNPPQNSQ LGRGVHELLL SQYKITFRTF PLSGKVTDFL
     801  SQHQNSIKNI LKTSTSSMDP VIESTGSSFL DKKSLLVAFE EVINQEEKSR
     851  ELLSGFKVKL AGFGPSRVGC NILLSQDNLL GSLFEGTPAA FEYSDSIKNG
     901  FQLAVSEGPL ANEPVQGMCV LVESVHKMSQ DEIESIEDPR YQQHIVDLSG
     951  RLITSTRDAI HEAFLDWSPR IMWAIYSCDI QTSVDVLGKV YAVILQRHGK
    1001  IISEEMKEGT PFFQIEAHVP VVEAFGLSED IRKRTSGAAQ PQLVFSGFEC
    1051  IDLDPFWVPT TEEELEELGD TADRENIARK HMNAIRRRKG LFIEEKVVEN
    1101  AEKQRTLKKN *

external links for Ria1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Ria1p

1)	Becam AM, et al. (2001) Ria1p (Ynl163c), a protein similar to elongation factors 2, is involved in the biogenesis of the 60S subunit of the ribosome in Saccharomyces cerevisiae. Mol Genet Genomics 266(3):454-62
2)	Senger B, et al. (2001) The nucle(ol)ar Tif6p and Efl1p are required for a late cytoplasmic step of ribosome synthesis. Mol Cell 8(6):1363-73
3)	Menne TF, et al. (2007) The Shwachman-Bodian-Diamond syndrome protein mediates translational activation of ribosomes in yeast. Nat Genet 39(4):486-95
4)	Gartmann M, et al. (2010) Mechanism of eIF6-mediated inhibition of ribosomal subunit joining. J Biol Chem 285(20):14848-51
5)	Finch AJ, et al. (2011) Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome. Genes Dev 25(9):917-29
6)	Bussiere C, et al. (2012) Integrity of the P-site is probed during maturation of the 60S ribosomal subunit. J Cell Biol 197(6):747-59
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41