YCK2/YNL154C Protein Information Help

Standard Name Yck2p
Systematic Name Ynl154cp
ORF Classification Verified
Description Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing; stabilized by Sod1p binding in the presence of glucose and oxygen, causing glucose repression of respiratory metabolism; YCK2 has a paralog, YCK1, that arose from the whole genome duplication (1, 10, 11, 2, 3, 4, 5, 6, 7, 8, 9)
Name Description Yeast Casein Kinase 5
Experimental Data
Molecules/cell 6160 12
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 546
Molecular Weight (Da) 62,079
Isoelectric Point (pI) 9.86

Click on image for expanded interactive view
pbrowse

Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Yck2p (InterPro)
Physical Interactions There are 253 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000127A60 | P23292
MIPS: YNL154C
NCBI: 1050798 | 1302114 | 140437 | 173210 | 433622 | 6324175 | NP_014245.1
GenBank/EMBL/DDBJ: DAA10395.1 | M74453 | X60326 | X92517 | Z71430
External Classifications EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSQVQSPLTA TNSGLAVNNN TMNSQMPNRS NVRLVNGTLP PSLHVSSNLN
      51  HNTGNSSASY SGSQSRDDST IVGLHYKIGK KIGEGSFGVL FEGTNMINGL
     101  PVAIKFEPRK TEAPQLKDEY RTYKILAGTP GIPQEYYFGQ EGLHNILVID
     151  LLGPSLEDLF DWCGRRFSVK TVVQVAVQMI TLIEDLHAHD LIYRDIKPDN
     201  FLIGRPGQPD ANKVHLIDFG MAKQYRDPKT KQHIPYREKK SLSGTARYMS
     251  INTHLGREQS RRDDMEAMGH VFFYFLRGQL PWQGLKAPNN KQKYEKIGEK
     301  KRLTNVYDLA QGLPIQFGRY LEIVRNLSFE ETPDYEGYRM LLLSVLDDLG
     351  ETADGQYDWM KLNGGRGWDL SINKKPNLHG YGHPNPPNEK SKRHRSKNHQ
     401  YSSPDHHHHY NQQQQQQQAQ AQAQAQAQAK VQQQQLQQAQ AQQQANRYQL
     451  QPDDSHYDEE REASKLDPTS YEAYQQQTQQ KYAQQQQKQM QQKSKQFANT
     501  GANGQTNKYP YNAQPTANDE QNAKNAAQDR NSNKSSKGFF SKLGCC*

external links for Yck2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Yck2p
1) Marchal C, et al.  (2000) Casein kinase I-dependent phosphorylation within a PEST sequence and ubiquitination at nearby lysines signal endocytosis of yeast uracil permease. J Biol Chem 275(31):23608-14
2) Hicke L, et al.  (1998) Cytoplasmic tail phosphorylation of the alpha-factor receptor is required for its ubiquitination and internalization. J Cell Biol 141(2):349-58
3) Wang PC, et al.  (1992) Two genes in Saccharomyces cerevisiae encode a membrane-bound form of casein kinase-1. Mol Biol Cell 3(3):275-86
4) Robinson LC, et al.  (1993) Casein kinase I-like protein kinases encoded by YCK1 and YCK2 are required for yeast morphogenesis. Mol Cell Biol 13(5):2870-81
5) Robinson LC, et al.  (1992) Yeast casein kinase I homologues: an essential gene pair. Proc Natl Acad Sci U S A 89(1):28-32
6) Robinson LC, et al.  (1999) The Yck2 yeast casein kinase 1 isoform shows cell cycle-specific localization to sites of polarized growth and is required for proper septin organization. Mol Biol Cell 10(4):1077-92
7) Feng Y and Davis NG  (2000) Akr1p and the type I casein kinases act prior to the ubiquitination step of yeast endocytosis: Akr1p is required for kinase localization to the plasma membrane. Mol Cell Biol 20(14):5350-9
8) Roth AF, et al.  (2002) The yeast DHHC cysteine-rich domain protein Akr1p is a palmitoyl transferase. J Cell Biol 159(1):23-8
9) Babu P, et al.  (2004) Akr1p-dependent palmitoylation of Yck2p yeast casein kinase 1 is necessary and sufficient for plasma membrane targeting. J Biol Chem 279(26):27138-47
10) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
11) Reddi AR and Culotta VC  (2013) SOD1 integrates signals from oxygen and glucose to repress respiration. Cell 152(1-2):224-35
12) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41