ALF1/YNL148C Protein Information

Standard Name	Alf1p
Systematic Name	Ynl148cp
ORF Classification	Verified
Description	Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance (1)
Name Description	ALpha-tubulin Foldin

Experimental Data
Molecules/cell	656 2

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	254
Molecular Weight (Da)	28,353
Isoelectric Point (pI)	4.6

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Alf1p (InterPro)
Physical Interactions	There are 9 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000136A4C \| P53904 MIPS: YNL148C NCBI: 1050804 \| 1302103 \| 1730786 \| 6324181 \| 854490 \| NP_014251.1 GenBank/EMBL/DDBJ: DAA10400.1 \| X92517 \| Z46843 \| Z71424

Amino Acid Sequence (or in FASTA format)
1 MVRVVIESEL VRTEKELPNS LKLRQFKDRL YHVTGVEPED MEIVVKRQYD 51 NKEIYSTKKG GAYSNEDEDA NFLKGEEELI VVVTDSNAQS ISNQLATQAE 101 GIPSMEVISE EDYLRRDQSV LRWKMAHGYG RFNAAQQSQR AALAKQDEAY 151 AREQLTAAIG RHCRVTVDGS APREAILRYV GPLPLDVMGT WCGVEFPEAA 201 GKNDGRINGV TLFGPVAPGH GSFVRPRAVE ILSKDEESAE VEDVHDDVES 251 DDEI*

Amino Acid Sequence (or in FASTA format)

       1  MVRVVIESEL VRTEKELPNS LKLRQFKDRL YHVTGVEPED MEIVVKRQYD
      51  NKEIYSTKKG GAYSNEDEDA NFLKGEEELI VVVTDSNAQS ISNQLATQAE
     101  GIPSMEVISE EDYLRRDQSV LRWKMAHGYG RFNAAQQSQR AALAKQDEAY
     151  AREQLTAAIG RHCRVTVDGS APREAILRYV GPLPLDVMGT WCGVEFPEAA
     201  GKNDGRINGV TLFGPVAPGH GSFVRPRAVE ILSKDEESAE VEDVHDDVES
     251  DDEI*

external links for Alf1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Alf1p

1)	Feierbach B, et al. (1999) Alf1p, a CLIP-170 domain-containing protein, is functionally and physically associated with alpha-tubulin. J Cell Biol 144(1):113-24
2)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41