LEU4/YNL104C Protein Information Help

Standard Name Leu4p 1
Systematic Name Ynl104cp
ORF Classification Verified
Description Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication (2, 3, 4)
Name Description LEUcine biosynthesis 1, 5
Experimental Data
Molecules/cell 6630 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 619
Molecular Weight (Da) 68,408
Isoelectric Point (pI) 5.89

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Leu4p (InterPro)
Physical Interactions There are 20 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012E4DF | P06208
MIPS: YNL104C
NCBI: 126192 | 1302020 | 171841 | 171842 | 3931 | 6324225 | 929849 | NP_014295.1 | NM_001182942.1
GenBank/EMBL/DDBJ: DAA10441.1 | M12893 | Z12126 | Z50161 | Z71380
External Classifications EC: 2.3.3.13 [2-isopropylmalate synthase]
Amino Acid Sequence (or in FASTA format)
 
       1  MVKESIIALA EHAASRASRV IPPVKLAYKN MLKDPSSKYK PFNAPKLSNR
      51  KWPDNRITRA PRWLSTDLRD GNQSLPDPMS VEQKKEYFHK LVNIGFKEIE
     101  VSFPSASQTD FDFTRYAVEN APDDVSIQCL VQSREHLIKR TVEALTGAKK
     151  ATIHTYLATS DMFREIVFNM SREEAISKAV EATKLVRKLT KDDPSQQATR
     201  WSYEFSPECF SDTPGEFAVE ICEAVKKAWE PTEENPIIFN LPATVEVASP
     251  NVYADQIEYF ATHITEREKV CISTHCHNDR GCGVAATELG MLAGADRVEG
     301  CLFGNGERTG NVDLVTVAMN MYTQGVSPNL DFSDLTSVLD VVERCNKIPV
     351  SQRAPYGGDL VVCAFSGSHQ DAIKKGFNLQ NKKRAQGETQ WRIPYLPLDP
     401  KDIGRDYEAV IRVNSQSGKG GAAWVILRSL GLDLPRNMQI EFSSAVQDHA
     451  DSLGRELKSD EISKLFKEAY NYNDEQYQAI SLVNYNVEKF GTERRVFTGQ
     501  VKVGDQIVDI EGTGNGPISS LVDALSNLLN VRFAVANYTE HSLGSGSSTQ
     551  AASYIHLSYR RNADNEKAYK WGVGVSEDVG DSSVRAIFAT INNIIHSGDV
     601  SIPSLAEVEG KNAAASGSA*

external links for Leu4p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Leu4p
1) Baichwal V, et al.  (1983) Leucine biosynthesis in yeast. Identification of two genes (LEU4, LEU5) that affect alpha-Isopropylmalate synthase activity and evidence that LEU1 and LEU2 gene expression is controlled by alpha-Isopropylmalate and the product of a regulatory gene. Curr Genet 7(5):369-377
2) Beltzer JP, et al.  (1988) Yeast LEU4 encodes mitochondrial and nonmitochondrial forms of alpha-isopropylmalate synthase. J Biol Chem 263(1):368-74
3) Drain P and Schimmel P  (1988) Multiple new genes that determine activity for the first step of leucine biosynthesis in Saccharomyces cerevisiae. Genetics 119(1):13-20
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Chang LF, et al.  (1984) Cloning and characterization of yeast Leu4, one of two genes responsible for alpha-isopropylmalate synthesis. Genetics 108(1):91-106
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41