MET4/YNL103W Protein Information Help

Standard Name Met4p 1
Systematic Name Ynl103wp
ORF Classification Verified
Description Leucine-zipper transcriptional activator; responsible for regulation of sulfur amino acid pathway; requires different combinations of auxiliary factors Cbf1p, Met28p, Met31p and Met32p; feedforward loop exists in the regulation of genes controlled by Met4p and Met32p; can be ubiquitinated by ubiquitin ligase SCF-Met30p, is either degraded or maintained in an inactive state; regulates degradation of its own DNA-binding cofactors by targeting them to SCF-Met30p (2, 3)
Name Description METhionine requiring 1
Experimental Data
Molecules/cell 1300 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 672
Molecular Weight (Da) 74,301
Isoelectric Point (pI) 5.59

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Met4p (InterPro)
Physical Interactions There are 62 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00001683F5 | P32389
MIPS: YNL103W
NCBI: 1302018 | 171940 | 3930 | 398364833 | 417296 | 88984445 | 929850 | NP_014296.4
GenBank/EMBL/DDBJ: DAA10442.1 | M84455 | Z12126 | Z50161 | Z71379
Amino Acid Sequence (or in FASTA format)
 
       1  MKQEQSHEGD SYSTEFINLF GKDTATHPSS NNGANNNGMG STNSLDQFVA
      51  TASSSSSLVT SSENRRPLIG DVTNRGNTNL YDHAVTPEIL LEQLAYVDNF
     101  IPSLDNEFSN VDWNVNTTHN NANNNGADTF SSINANPFDL DEQLAIELSA
     151  FADDSFIFPD EDKPSNNNNN SNNGNDDHSN HDVLHEDPST NNRQRNPHFL
     201  TQRRNTFLTS QYDQSKSRFS SKNKRNGNNG ETNNFGDNMQ NNHPFEPNFM
     251  GSPSQFPADA TNMTSIDHGG FTNVDITSTE NNTTGDNGVD ALSNLLHRTT
     301  HTPNRSSPLS NVTSAQNSSS QQRKHSESKV DSNSDNNSSN KAPNITVPDY
     351  SIIPTSVLVT LLPRVNVPNG AYNSLISAGF DNDQIDAIAA IMAYHHQKKI
     401  RENNSNNNKN INTNDSQEAP ILKNINELLS VLIPPSPAET AAPTTLSTSP
     451  SFNEHGVVAE ASFLSSILEL GIKHPKSNNI HNQRQPSRND HKISRESDGN
     501  NGNDNVHHNN AVIKSSTTRG DEIAKIRSEP TLNASSSDHK ENSLKRSHSG
     551  DLKNKKVPVD RKYSDNEDDE YDDADLHGFE KKQLIKKELG DDDEDLLIQS
     601  KKSHQKKKLK EKELESSIHE LTEIAASLQK RIHTLETENK LLKNLVLSSG
     651  ETEGIKKAES LKKQIFEKVQ KE*

external links for Met4p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome

YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Met4p
1) Masselot M and De Robichon-Szulmajster H  (1975) Methionine biosynthesis in Saccharomyces cerevisiae. I. Genetical analysis of auxotrophic mutants. Mol Gen Genet 139(2):121-32
2) Blaiseau PL and Thomas D  (1998) Multiple transcriptional activation complexes tether the yeast activator Met4 to DNA. EMBO J 17(21):6327-36
3) McIsaac RS, et al.  (2012) Perturbation-based analysis and modeling of combinatorial regulation in the yeast sulfur assimilation pathway. Mol Biol Cell 23(15):2993-3007
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41