POL1/YNL102W Protein Information Help

Standard Name Pol1p 1
Systematic Name Ynl102wp
Alias Cdc17p 2 , Crt5p 3 , Hpr3p
ORF Classification Verified
Description Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis (4, 5, 6)
Name Description POLymerase
Experimental Data
Molecules/cell 1050 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,468
Molecular Weight (Da) 166,808
Isoelectric Point (pI) 6.14

Click on image for expanded interactive view
pbrowse

Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Pol1p (InterPro)
Physical Interactions There are 95 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052DF8 | P13382
MIPS: YNL102W
NCBI: 1302016 | 1706503 | 172209 | 239781864 | 239781867 | 239781870 | 239781873 | 310942889 | 3932 | 398364843 | 453055722 | 453056078 | 453056079 | 453056080 | 453056085 | 453056086 | 929851 | NP_014297.3
GenBank/EMBL/DDBJ: DAA10443.1 | J03268 | Z12126 | Z50161 | Z71378
External Classifications EC: 2.7.7.7 [DNA-directed DNA polymerase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSSKSEKLEK LRKLQAARNG TSIDDYEGDE SDGDRIYDEI DEKEYRARKR
      51  QELLHDDFVV DDDGVGYVDR GVEEDWREVD NSSSDEDTGN LASKDSKRKK
     101  NIKREKDHQI TDMLRTQHSK STLLAHAKKS QKKSIPIDNF DDILGEFESG
     151  EVEKPNILLP SKLRENLNSS PTSEFKSSIK RVNGNDESSH DAGISKKVKI
     201  DPDSSTDKYL EIESSPLKLQ SRKLRYANDV QDLLDDVENS PVVATKRQNV
     251  LQDTLLANPP SAQSLADEED DEDSDEDIIL KRRTMRSVTT TRRVNIDSRS
     301  NPSTSPFVTA PGTPIGIKGL TPSKSLQSNT DVATLAVNVK KEDVVDPETD
     351  TFQMFWLDYC EVNNTLILFG KVKLKDDNCV SAMVQINGLC RELFFLPREG
     401  KTPTDIHEEI IPLLMDKYGL DNIRAKPQKM KYSFELPDIP SESDYLKVLL
     451  PYQTPKSSRD TIPSDLSSDT FYHVFGGNSN IFESFVIQNR IMGPCWLDIK
     501  GADFNSIRNA SHCAVEVSVD KPQNITPTTT KTMPNLRCLS LSIQTLMNPK
     551  ENKQEIVSIT LSAYRNISLD SPIPENIKPD DLCTLVRPPQ STSFPLGLAA
     601  LAKQKLPGRV RLFNNEKAML SCFCAMLKVE DPDVIIGHRL QNVYLDVLAH
     651  RMHDLNIPTF SSIGRRLRRT WPEKFGRGNS NMNHFFISDI CSGRLICDIA
     701  NEMGQSLTPK CQSWDLSEMY QVTCEKEHKP LDIDYQNPQY QNDVNSMTMA
     751  LQENITNCMI SAEVSYRIQL LTLTKQLTNL AGNAWAQTLG GTRAGRNEYI
     801  LLHEFSRNGF IVPDKEGNRS RAQKQRQNEE NADAPVNSKK AKYQGGLVFE
     851  PEKGLHKNYV LVMDFNSLYP SIIQEFNICF TTVDRNKEDI DELPSVPPSE
     901  VDQGVLPRLL ANLVDRRREV KKVMKTETDP HKRVQCDIRQ QALKLTANSM
     951  YGCLGYVNSR FYAKPLAMLV TNKGREILMN TRQLAESMNL LVVYGDTDSV
    1001  MIDTGCDNYA DAIKIGLGFK RLVNERYRLL EIDIDNVFKK LLLHAKKKYA
    1051  ALTVNLDKNG NGTTVLEVKG LDMKRREFCP LSRDVSIHVL NTILSDKDPE
    1101  EALQEVYDYL EDIRIKVETN NIRIDKYKIN MKLSKDPKAY PGGKNMPAVQ
    1151  VALRMRKAGR VVKAGSVITF VITKQDEIDN AADTPALSVA ERAHALNEVM
    1201  IKSNNLIPDP QYYLEKQIFA PVERLLERID SFNVVRLSEA LGLDSKKYFR
    1251  REGGNNNGED INNLQPLETT ITDVERFKDT VTLELSCPSC DKRFPFGGIV
    1301  SSNYYRVSYN GLQCKHCEQL FTPLQLTSQI EHSIRAHISL YYAGWLQCDD
    1351  STCGIVTRQV SVFGKRCLND GCTGVMRYKY SDKQLYNQLL YFDSLFDCEK
    1401  NKKQELKPIY LPDDLDYPKE QLTESSIKAL TEQNRELMET GRSVVQKYLN
    1451  DCGRRYVDMT SIFDFMLN*

external links for Pol1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Pol1p
1) Burgers PM, et al.  (1990) Revised nomenclature for eukaryotic DNA polymerases. Eur J Biochem 191(3):617-8
2) Carson, M.J.  (1987) CDC17, the structural gene for DNA polymerase I of yeast: mitotic hyperrecombination and effects on telomere metabolism. Ph.D. thesis
3) Zhou Z and Elledge SJ  (1992) Isolation of crt mutants constitutive for transcription of the DNA damage inducible gene RNR3 in Saccharomyces cerevisiae. Genetics 131(4):851-66
4) Foiani M, et al.  (1996) A meiosis-specific protein kinase, Ime2, is required for the correct timing of DNA replication and for spore formation in yeast meiosis. Mol Gen Genet 253(3):278-88
5) Burgers PM  (1998) Eukaryotic DNA polymerases in DNA replication and DNA repair. Chromosoma 107(4):218-27
6) Gutierrez PJ and Wang TS  (2003) Genomic instability induced by mutations in Saccharomyces cerevisiae POL1. Genetics 165(1):65-81
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41