TOP2/YNL088W Protein Information Help

Standard Name Top2p 1
Systematic Name Ynl088wp
Alias Tor3p , Trf3p 2
ORF Classification Verified
Description Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation (3, 4, 5, 6, 7)
Name Description TOPoisomerase 1, 8
Experimental Data
Molecules/cell 5730 9
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,428
Molecular Weight (Da) 164,213
Isoelectric Point (pI) 7.03

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Top2p (InterPro)
Physical Interactions There are 71 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00000010F4 | P06786
MIPS: YNL088W
NCBI: 1301989 | 1351262 | 157830305 | 157830349 | 163931058 | 172998 | 19880866 | 19880880 | 19880887 | 297039743 | 297039748 | 34810356 | 34810357 | 37928124 | 37928125 | 398364937 | 410945796 | 410945801 | 887623 | NP_014311.3
GenBank/EMBL/DDBJ: DAA10457.1 | AF458969 | AF458971 | AF458972 | M13814 | X89016 | Z71364
External Classifications EC: 5.99.1.3 [DNA topoisomerase (ATP-hydrolyzing)]
Amino Acid Sequence (or in FASTA format)
 
       1  MSTEPVSASD KYQKISQLEH ILKRPDTYIG SVETQEQLQW IYDEETDCMI
      51  EKNVTIVPGL FKIFDEILVN AADNKVRDPS MKRIDVNIHA EEHTIEVKND
     101  GKGIPIEIHN KENIYIPEMI FGHLLTSSNY DDDEKKVTGG RNGYGAKLCN
     151  IFSTEFILET ADLNVGQKYV QKWENNMSIC HPPKITSYKK GPSYTKVTFK
     201  PDLTRFGMKE LDNDILGVMR RRVYDINGSV RDINVYLNGK SLKIRNFKNY
     251  VELYLKSLEK KRQLDNGEDG AAKSDIPTIL YERINNRWEV AFAVSDISFQ
     301  QISFVNSIAT TMGGTHVNYI TDQIVKKISE ILKKKKKKSV KSFQIKNNMF
     351  IFINCLIENP AFTSQTKEQL TTRVKDFGSR CEIPLEYINK IMKTDLATRM
     401  FEIADANEEN ALKKSDGTRK SRITNYPKLE DANKAGTKEG YKCTLVLTEG
     451  DSALSLAVAG LAVVGRDYYG CYPLRGKMLN VREASADQIL KNAEIQAIKK
     501  IMGLQHRKKY EDTKSLRYGH LMIMTDQDHD GSHIKGLIIN FLESSFPGLL
     551  DIQGFLLEFI TPIIKVSITK PTKNTIAFYN MPDYEKWREE ESHKFTWKQK
     601  YYKGLGTSLA QEVREYFSNL DRHLKIFHSL QGNDKDYIDL AFSKKKADDR
     651  KEWLRQYEPG TVLDPTLKEI PISDFINKEL ILFSLADNIR SIPNVLDGFK
     701  PGQRKVLYGC FKKNLKSELK VAQLAPYVSE CTAYHHGEQS LAQTIIGLAQ
     751  NFVGSNNIYL LLPNGAFGTR ATGGKDAAAA RYIYTELNKL TRKIFHPADD
     801  PLYKYIQEDE KTVEPEWYLP ILPMILVNGA EGIGTGWSTY IPPFNPLEII
     851  KNIRHLMNDE ELEQMHPWFR GWTGTIEEIE PLRYRMYGRI EQIGDNVLEI
     901  TELPARTWTS TIKEYLLLGL SGNDKIKPWI KDMEEQHDDN IKFIITLSPE
     951  EMAKTRKIGF YERFKLISPI SLMNMVAFDP HGKIKKYNSV NEILSEFYYV
    1001  RLEYYQKRKD HMSERLQWEV EKYSFQVKFI KMIIEKELTV TNKPRNAIIQ
    1051  ELENLGFPRF NKEGKPYYGS PNDEIAEQIN DVKGATSDEE DEESSHEDTE
    1101  NVINGPEELY GTYEYLLGMR IWSLTKERYQ KLLKQKQEKE TELENLLKLS
    1151  AKDIWNTDLK AFEVGYQEFL QRDAEARGGN VPNKGSKTKG KGKRKLVDDE
    1201  DYDPSKKNKK STARKGKKIK LEDKNFERIL LEQKLVTKSK APTKIKKEKT
    1251  PSVSETKTEE EENAPSSTSS SSIFDIKKED KDEGELSKIS NKFKKISTIF
    1301  DKMGSTSATS KENTPEQDDV ATKKNQTTAK KTAVKPKLAK KPVRKQQKVV
    1351  ELSGESDLEI LDSYTDREDS NKDEDDAIPQ RSRRQRSSRA ASVPKKSYVE
    1401  TLELSDDSFI EDDEEENQGS DVSFNEED*

external links for Top2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Top2p
1) Holm C, et al.  (1985) DNA topoisomerase II is required at the time of mitosis in yeast. Cell 41(2):553-63
2) Sadoff BU, et al.  (1995) Isolation of mutants of Saccharomyces cerevisiae requiring DNA topoisomerase I. Genetics 141(2):465-79
3) Klein F, et al.  (1992) Localization of RAP1 and topoisomerase II in nuclei and meiotic chromosomes of yeast. J Cell Biol 117(5):935-48
4) Goto T and Wang JC  (1984) Yeast DNA topoisomerase II is encoded by a single-copy, essential gene. Cell 36(4):1073-80
5) Wang JC  (1996) DNA topoisomerases. Annu Rev Biochem 65:635-92
6) Roca J  (1995) The mechanisms of DNA topoisomerases. Trends Biochem Sci 20(4):156-60
7) Hashash N, et al.  (2012) Topoisomerase II- and Condensin-Dependent Breakage of MEC1(ATR)-Sensitive Fragile Sites Occurs Independently of Spindle Tension, Anaphase, or Cytokinesis. PLoS Genet 8(10):e1002978
8) DiNardo S, et al.  (1984) DNA topoisomerase II mutant of Saccharomyces cerevisiae: topoisomerase II is required for segregation of daughter molecules at the termination of DNA replication. Proc Natl Acad Sci U S A 81(9):2616-20
9) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41