RNH201/YNL072W Protein Information

Standard Name	Rnh201p
Systematic Name	Ynl072wp
Alias	Rnh35p 1
ORF Classification	Verified
Description	Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome (1, 2, 3, 4)
Name Description	RNase H 1
Gene Product	Rnh2A

Experimental Data
Molecules/cell	623 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	307
Molecular Weight (Da)	34,875
Isoelectric Point (pI)	9.62

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rnh201p (InterPro)
Physical Interactions	There are 7 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001343E9 \| P53942 MIPS: YNL072W NCBI: 1301957 \| 1730743 \| 6324257 \| 791114 \| NP_014327.1 GenBank/EMBL/DDBJ: DAA10473.1 \| X86470 \| Z71348
External Classifications	EC: 3.1.26.4 [Ribonuclease H]

Amino Acid Sequence (or in FASTA format)
1 MVPPTVEASL ESPYTKSYFS PVPSALLEQN DSPIIMGIDE AGRGPVLGPM 51 VYAVAYSTQK YQDETIIPNY EFDDSKKLTD PIRRMLFSKI YQDNEELTQI 101 GYATTCITPL DISRGMSKFP PTRNYNLNEQ AHDVTMALID GVIKQNVKLS 151 HVYVDTVGPP ASYQKKLEQR FPGVKFTVAK KADSLYCMVS VASVVAKVTR 201 DILVESLKRD PDEILGSGYP SDPKTVAWLK RNQTSLMGWP ANMVRFSWQT 251 CQTLLDDASK NSIPIKWEEQ YMDSRKNAAQ KTKQLQLQMV AKPVRRKRLR 301 TLDNWYR*

Amino Acid Sequence (or in FASTA format)

       1  MVPPTVEASL ESPYTKSYFS PVPSALLEQN DSPIIMGIDE AGRGPVLGPM
      51  VYAVAYSTQK YQDETIIPNY EFDDSKKLTD PIRRMLFSKI YQDNEELTQI
     101  GYATTCITPL DISRGMSKFP PTRNYNLNEQ AHDVTMALID GVIKQNVKLS
     151  HVYVDTVGPP ASYQKKLEQR FPGVKFTVAK KADSLYCMVS VASVVAKVTR
     201  DILVESLKRD PDEILGSGYP SDPKTVAWLK RNQTSLMGWP ANMVRFSWQT
     251  CQTLLDDASK NSIPIKWEEQ YMDSRKNAAQ KTKQLQLQMV AKPVRRKRLR
     301  TLDNWYR*

external links for Rnh201p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Rnh201p

1)	Frank P, et al. (1998) Yeast RNase H(35) is the counterpart of the mammalian RNase HI, and is evolutionarily related to prokaryotic RNase HII. FEBS Lett 421(1):23-6
2)	Qiu J, et al. (1999) Saccharomyces cerevisiae RNase H(35) functions in RNA primer removal during lagging-strand DNA synthesis, most efficiently in cooperation with Rad27 nuclease. Mol Cell Biol 19(12):8361-71
3)	Crow YJ, et al. (2006) Mutations in genes encoding ribonuclease H2 subunits cause Aicardi-Goutieres syndrome and mimic congenital viral brain infection. Nat Genet 38(8):910-6
4)	Nick McElhinny SA, et al. (2010) Genome instability due to ribonucleotide incorporation into DNA. Nat Chem Biol 6(10):774-81
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41