RNH201/YNL072W Protein Information Help

Standard Name Rnh201p 1
Systematic Name Ynl072wp
Alias Rnh35p 2
ORF Classification Verified
Description Ribonuclease H2 catalytic subunit; removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; role in ribonucleotide excision repair; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome (1, 2, 3, 4, 5)
Name Description RNase H 2
Gene Product Rnh2A
Experimental Data
Molecules/cell 623 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 307
Molecular Weight (Da) 34,875
Isoelectric Point (pI) 9.62

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Rnh201p (InterPro)
Physical Interactions There are 7 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00001343E9 | P53942
MIPS: YNL072W
NCBI: 1301957 | 1730743 | 6324257 | 791114 | NP_014327.1 | NM_001182910.1
GenBank/EMBL/DDBJ: CAA60188.1 | CAA95946.1 | DAA10473.1 | X86470 | Z71348
External Classifications EC: 3.1.26.4 [Ribonuclease H]
Amino Acid Sequence (or in FASTA format)
       1  MVPPTVEASL ESPYTKSYFS PVPSALLEQN DSPIIMGIDE AGRGPVLGPM
      51  VYAVAYSTQK YQDETIIPNY EFDDSKKLTD PIRRMLFSKI YQDNEELTQI
     101  GYATTCITPL DISRGMSKFP PTRNYNLNEQ AHDVTMALID GVIKQNVKLS
     151  HVYVDTVGPP ASYQKKLEQR FPGVKFTVAK KADSLYCMVS VASVVAKVTR
     201  DILVESLKRD PDEILGSGYP SDPKTVAWLK RNQTSLMGWP ANMVRFSWQT
     251  CQTLLDDASK NSIPIKWEEQ YMDSRKNAAQ KTKQLQLQMV AKPVRRKRLR
     301  TLDNWYR*                                              

external links for Rnh201p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) Organelle DB
Candida (CGD) CYC2008 (complexes) MIPS YPL+
YGOB Complexome Pfam domains YeastGFP
YOGY DIP YeastRC Structure Prediction (Seattle) YeastRC Public Image Repository

GeneMANIA


IMP

References cited on this page View Complete Literature Guide for Rnh201p
1) Nick McElhinny SA, et al.  (2010) Genome instability due to ribonucleotide incorporation into DNA. Nat Chem Biol 6(10):774-81
2) Frank P, et al.  (1998) Yeast RNase H(35) is the counterpart of the mammalian RNase HI, and is evolutionarily related to prokaryotic RNase HII. FEBS Lett 421(1):23-6
3) Qiu J, et al.  (1999) Saccharomyces cerevisiae RNase H(35) functions in RNA primer removal during lagging-strand DNA synthesis, most efficiently in cooperation with Rad27 nuclease. Mol Cell Biol 19(12):8361-71
4) Crow YJ, et al.  (2006) Mutations in genes encoding ribonuclease H2 subunits cause Aicardi-Goutieres syndrome and mimic congenital viral brain infection. Nat Genet 38(8):910-6
5) Sparks JL, et al.  (2012) RNase H2-initiated ribonucleotide excision repair. Mol Cell 47(6):980-6
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41