NOP2/YNL061W Protein Information Help

Standard Name Nop2p
Systematic Name Ynl061wp
Alias Yna1p
ORF Classification Verified
Description Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles (1, 2, 3, 4)
Name Description NucleOlar Protein 1
Experimental Data
Molecules/cell 18700 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 618
Molecular Weight (Da) 69,812
Isoelectric Point (pI) 4.79

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Nop2p (InterPro)
Physical Interactions There are 214 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000053116 | P40991
MIPS: YNL061W
NCBI: 1098483 | 1301934 | 398365157 | 51013571 | 576442 | 603587 | 730166 | NP_014338.3
GenBank/EMBL/DDBJ: DAA10485.1 | AY693060 | U12141 | X82656 | X83512 | Z71337
External Classifications EC: 2.1.1.- [Transferases transferring one-carbon groups methyltransferases]
Amino Acid Sequence (or in FASTA format)
 
       1  MGSRRHKNKQ AAPPTLEEFQ ARKEKKANRK LEKGKRPSTT QGDEVSDRKK
      51  KKSKPFKKSR KEEEEVVEED KDLPEVDLEE LSKARKSLFD DEEDDDEAGL
     101  VDEELKDEFD LEQEYDYDED EDNDAHPIFS DDDDEADLEE LNAQNMEALS
     151  KKLDEEEAEE AEEAEMELVE AENMQPRADI LPTEEQEEMM AQETPNLTST
     201  RTRMIEIVKV LENFKTLGAE GRSRGEYVDR LLKDICEYFG YTPFLAEKLF
     251  NLFSPAEAME FFEANEIARP ITIRTNTLKT RRRDLAQTLV NRGVNLQPIG
     301  SWTKVGLQIF DSQVPIGATP EYLAGHYILQ AASSFLPVIA LDPHENERIL
     351  DMAAAPGGKT TYISAMMKNT GCVFANDANK SRTKSLIANI HRLGCTNTIV
     401  CNYDAREFPK VIGGFDRILL DAPCSGTGVI GKDQSVKVSR TEKDFIQIPH
     451  LQKQLLLSAI DSVDCNSKHG GVIVYSTCSV AVEEDEAVID YALRKRPNVK
     501  LVDTGLAIGK EAFTSYRGKK FHPSVKLARR YYPHTYNVDG FFVAKFQKIG
     551  PSSFDDNQAS AKEKETAARK EALEEGIIHS DFATFEDEED DKYIEKSVKN
     601  NLLKKGVNPK AKRPSNEK*

external links for Nop2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Nop2p
1) de Beus E, et al.  (1994) Yeast NOP2 encodes an essential nucleolar protein with homology to a human proliferation marker. J Cell Biol 127(6 Pt 2):1799-813
2) King M, et al.  (1999) A conserved motif in the yeast nucleolar protein Nop2p contains an essential cysteine residue. Biochem J 337 ( Pt 1)():29-35
3) Hong B, et al.  (2001) Temperature sensitive nop2 alleles defective in synthesis of 25S rRNA and large ribosomal subunits in Saccharomyces cerevisiae. Nucleic Acids Res 29(14):2927-37
4) Horsey EW, et al.  (2004) Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation. RNA 10(5):813-27
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41