SAM50/YNL026W Protein Information

Standard Name	Sam50p 1
Systematic Name	Ynl026wp
Alias	Omp85p 2 , Tob55p 3
ORF Classification	Verified
Description	Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 (1, 2, 3, 4)
Name Description	Sorting and Assembly Machinery 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	484
Molecular Weight (Da)	54,406
Isoelectric Point (pI)	8.68

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Sam50p (InterPro)
Physical Interactions	There are 24 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013BAF3 \| P53969 MIPS: YNL026W NCBI: 1730715 \| 6324302 \| NP_014372.1 GenBank/EMBL/DDBJ: DAA10518.1 \| Z71302

Amino Acid Sequence (or in FASTA format)
1 MTSSSGVDNE ISLDSPMPIF NESSTLKPIR VAGVVTTGTD HIDPSVLQAY 51 LDDTIMKSIT LGQLVKNADV LNKRLCQHHI ALNAKQSFHF QGNTYISDEK 101 ETHDVVPLME VVSQLDILPP KTFTAKTGTN FGNDNDAEAY LQFEKLIDKK 151 YLKLPTRVNL EILRGTKIHS SFLFNSYSSL SPQSILNLKV FSQFYNWNTN 201 KGLDIGQRGA RLSLRYEPLF LHKLLHNPHS NESPTLFHEW FLETCWRSTK 251 ICSQGTSAPY MYSGTMLSQA GDQLRTILGH TFVLDKRDHI MCPTKGSMLK 301 WSNELSPGKH LKTQLELNSV KSWMNDDFIT FSTTIKTGYL KNLSSQQSLP 351 VHICDKFQSG GPSDIRGFQT FGLGPRDLYD AVGGDAFVSY GLSVFSRLPW 401 KKVEKSNFRL HWFFNGGKLV NHDNTSLGNC IGQLSKEHST STGIGLVLRH 451 PMARFELNFT LPITAHENDL IRKGFQFGLG LAFL*

Amino Acid Sequence (or in FASTA format)

       1  MTSSSGVDNE ISLDSPMPIF NESSTLKPIR VAGVVTTGTD HIDPSVLQAY
      51  LDDTIMKSIT LGQLVKNADV LNKRLCQHHI ALNAKQSFHF QGNTYISDEK
     101  ETHDVVPLME VVSQLDILPP KTFTAKTGTN FGNDNDAEAY LQFEKLIDKK
     151  YLKLPTRVNL EILRGTKIHS SFLFNSYSSL SPQSILNLKV FSQFYNWNTN
     201  KGLDIGQRGA RLSLRYEPLF LHKLLHNPHS NESPTLFHEW FLETCWRSTK
     251  ICSQGTSAPY MYSGTMLSQA GDQLRTILGH TFVLDKRDHI MCPTKGSMLK
     301  WSNELSPGKH LKTQLELNSV KSWMNDDFIT FSTTIKTGYL KNLSSQQSLP
     351  VHICDKFQSG GPSDIRGFQT FGLGPRDLYD AVGGDAFVSY GLSVFSRLPW
     401  KKVEKSNFRL HWFFNGGKLV NHDNTSLGNC IGQLSKEHST STGIGLVLRH
     451  PMARFELNFT LPITAHENDL IRKGFQFGLG LAFL*

external links for Sam50p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Sam50p

1)	Kozjak V, et al. (2003) An essential role of Sam50 in the protein sorting and assembly machinery of the mitochondrial outer membrane. J Biol Chem 278(49):48520-3
2)	Gentle I, et al. (2004) The Omp85 family of proteins is essential for outer membrane biogenesis in mitochondria and bacteria. J Cell Biol 164(1):19-24
3)	Paschen SA, et al. (2003) Evolutionary conservation of biogenesis of beta-barrel membrane proteins. Nature 426(6968):862-6
4)	Habib SJ, et al. (2005) Assembly of the TOB complex of mitochondria. J Biol Chem 280(8):6434-40