RCM1/YNL022C Protein Information Help

Standard Name Rcm1p 1, 2
Systematic Name Ynl022cp
ORF Classification Verified
Description Putative cytosine 5-methyltransferase, interacts with Trm112p; contains seven beta-strand methyltransferase motif similar to NOP2/YNL061W; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; predicted to be involved in ribosome biogenesis (2, 3, 4, 5, 6, 7)
Experimental Data
Molecules/cell 922 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 490
Molecular Weight (Da) 56,177
Isoelectric Point (pI) 9.03

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Rcm1p (InterPro)
Physical Interactions There are 15 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013BAEE | P53972
MIPS: YNL022C
NCBI: 1301853 | 1730712 | 398365407 | NP_014376.3 | NM_001182861.3
GenBank/EMBL/DDBJ: DAA10523.1 | Z71298
Amino Acid Sequence (or in FASTA format)
 
       1  MNFYRDATWV LEDIEKEAAK ERISGSMQTL VLKSCKRYKL KSNPKHIYAV
      51  LDSCWKYKPY LEKVMKKAHI LEDIPKKKGK PLFSRLTLLL LCHDLLLSKQ
     101  KRIQMGKHPI KDYVLKFKSP LHSEMVKLKL KLKVRELSEL VLSEDISNDL
     151  PPVRWIRINP LKCHPNGETE PVLAELRKKF TLKVDKWSEL VPGSIYYDEF
     201  IPNLFGIHPS DKITAHELYK HGKIIIQDRA SCFPAHILNP GPSDIVIDSC
     251  SAPGNKTTHT ASYIYPEPPK DNNTRIYAFE KDPERAKVLQ KMIKIAGCSP
     301  NISVNVGDFT KLATPEKYKD VTCFIVDPSC SGSGIFGRKF FDSFNRRKID
     351  DKDDDGGIVP DEQEEFIAKE ELQTRLAKLS SFQFQMVKHA MSFPAAKKIV
     401  YSTCSIHAEE NERVVIDLLL DKSVREWGWK VAPKREVIPS WPRRGKVEEF
     451  EEVFRDGVTY DPQQLAEGCI RALPKSDGGI GFFAVCFERD *

external links for Rcm1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Rcm1p
1) Kos M  (2010) personal communication
2) Sardana R and Johnson AW  (2012) The methyltransferase adaptor protein Trm112 is involved in biogenesis of both ribosomal subunits. Mol Biol Cell 23(21):4313-22
3) Brachat A, et al.  (2000) Analysis of deletion phenotypes and GFP fusions of 21 novel Saccharomyces cerevisiae open reading frames. Yeast 16(3):241-53
4) Giaever G, et al.  (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91
5) Kalhor HR, et al.  (2005) A novel methyltransferase required for the formation of the hypermodified nucleoside wybutosine in eucaryotic tRNA. Biochem Biophys Res Commun 334(2):433-40
6) Wade CH, et al.  (2006) The budding yeast rRNA and ribosome biosynthesis (RRB) regulon contains over 200 genes. Yeast 23(4):293-306
7) Petrossian TC and Clarke SG  (2009) Multiple Motif Scanning to identify methyltransferases from the yeast proteome. Mol Cell Proteomics 8(7):1516-26
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41