PUB1/YNL016W Protein Information Help

Standard Name Pub1p 1
Systematic Name Ynl016wp
Alias Rnp1p 2
ORF Classification Verified
Description Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress (1, 3, 4, 5, 6)
Name Description PolyUridylate Binding 1
Experimental Data
Molecules/cell 49600 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 453
Molecular Weight (Da) 50,763
Isoelectric Point (pI) 4.81

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Pub1p (InterPro)
Physical Interactions There are 52 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00000532A0 | P32588
MIPS: YNL016W
NCBI: 1301841 | 172438 | 295646 | 295789538 | 295789539 | 295789540 | 308153665 | 311124 | 347948469 | 417556 | 6324312 | NP_014382.1
GenBank/EMBL/DDBJ: DAA10528.1 | L01797 | L13725 | L20767 | Z71292
Amino Acid Sequence (or in FASTA format)
 
       1  MSENNEEQHQ QQQQQQPVAV ETPSAVEAPA SADPSSEQSV AVEGNSEQAE
      51  DNQGENDPSV VPANAITGGR ETSDRVLYVG NLDKAITEDI LKQYFQVGGP
     101  IANIKIMIDK NNKNVNYAFV EYHQSHDANI ALQTLNGKQI ENNIVKINWA
     151  FQSQQSSSDD TFNLFVGDLN VNVDDETLRN AFKDFPSYLS GHVMWDMQTG
     201  SSRGYGFVSF TSQDDAQNAM DSMQGQDLNG RPLRINWAAK RDNNNNNNYQ
     251  QRRNYGNNNR GGFRQYNSNN NNNMNMGMNM NMNMNMNNSR GMPPSSMGMP
     301  IGAMPLPSQG QPQQSQTIGL PPQVNPQAVD HIIRSAPPRV TTAYIGNIPH
     351  FATEADLIPL FQNFGFILDF KHYPEKGCCF IKYDTHEQAA VCIVALANFP
     401  FQGRNLRTGW GKERSNFMPQ QQQQGGQPLI MNDQQQPVMS EQQQQQQQQQ
     451  QQQ*

external links for Pub1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Pub1p
1) Matunis MJ, et al.  (1993) PUB1: a major yeast poly(A)+ RNA-binding protein. Mol Cell Biol 13(10):6114-23
2) Ripmaster TL and Woolford JL Jr  (1993) A protein containing conserved RNA-recognition motifs is associated with ribosomal subunits in Saccharomyces cerevisiae. Nucleic Acids Res 21(14):3211-6
3) Anderson JT, et al.  (1993) PUB1 is a major nuclear and cytoplasmic polyadenylated RNA-binding protein in Saccharomyces cerevisiae. Mol Cell Biol 13(10):6102-13
4) Duttagupta R, et al.  (2005) Global analysis of Pub1p targets reveals a coordinate control of gene expression through modulation of binding and stability. Mol Cell Biol 25(13):5499-513
5) Buchan JR, et al.  (2008) P bodies promote stress granule assembly in Saccharomyces cerevisiae. J Cell Biol 183(3):441-55
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41