SIS1/YNL007C Protein Information

Standard Name	Sis1p 1
Systematic Name	Ynl007cp
ORF Classification	Verified
Description	Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; has similarity to bacterial DnaJ proteins; protein abundance increases in response to DNA replication stress (2, 3, 4)
Name Description	SIt4 Suppressor 1

Experimental Data
Molecules/cell	20300 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	352
Molecular Weight (Da)	37,590
Isoelectric Point (pI)	9.86

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Sis1p (InterPro)
Physical Interactions	There are 78 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052E5F \| P25294 MIPS: YNL007C NCBI: 116666768 \| 116666769 \| 116666770 \| 122921354 \| 1301824 \| 134509 \| 4474 \| 6324321 \| 9954877 \| NP_014391.1 GenBank/EMBL/DDBJ: DAA10536.1 \| X58460 \| Z71283

Amino Acid Sequence (or in FASTA format)
1 MVKETKLYDL LGVSPSANEQ ELKKGYRKAA LKYHPDKPTG DTEKFKEISE 51 AFEILNDPQK REIYDQYGLE AARSGGPSFG PGGPGGAGGA GGFPGGAGGF 101 SGGHAFSNED AFNIFSQFFG GSSPFGGADD SGFSFSSYPS GGGAGMGGMP 151 GGMGGMHGGM GGMPGGFRSA SSSPTYPEEE TVQVNLPVSL EDLFVGKKKS 201 FKIGRKGPHG ASEKTQIDIQ LKPGWKAGTK ITYKNQGDYN PQTGRRKTLQ 251 FVIQEKSHPN FKRDGDDLIY TLPLSFKESL LGFSKTIQTI DGRTLPLSRV 301 QPVQPSQTST YPGQGMPTPK NPSQRGNLIV KYKVDYPISL NDAQKRAIDE 351 NF*

Amino Acid Sequence (or in FASTA format)

       1  MVKETKLYDL LGVSPSANEQ ELKKGYRKAA LKYHPDKPTG DTEKFKEISE
      51  AFEILNDPQK REIYDQYGLE AARSGGPSFG PGGPGGAGGA GGFPGGAGGF
     101  SGGHAFSNED AFNIFSQFFG GSSPFGGADD SGFSFSSYPS GGGAGMGGMP
     151  GGMGGMHGGM GGMPGGFRSA SSSPTYPEEE TVQVNLPVSL EDLFVGKKKS
     201  FKIGRKGPHG ASEKTQIDIQ LKPGWKAGTK ITYKNQGDYN PQTGRRKTLQ
     251  FVIQEKSHPN FKRDGDDLIY TLPLSFKESL LGFSKTIQTI DGRTLPLSRV
     301  QPVQPSQTST YPGQGMPTPK NPSQRGNLIV KYKVDYPISL NDAQKRAIDE
     351  NF*

external links for Sis1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Sis1p

1)	Luke MM, et al. (1991) Characterization of SIS1, a Saccharomyces cerevisiae homologue of bacterial dnaJ proteins. J Cell Biol 114(4):623-38
2)	Lu Z and Cyr DM (1998) Protein folding activity of Hsp70 is modified differentially by the hsp40 co-chaperones Sis1 and Ydj1. J Biol Chem 273(43):27824-30
3)	Fan CY, et al. (2004) Exchangeable chaperone modules contribute to specification of type I and type II Hsp40 cellular function. Mol Biol Cell 15(2):761-73
4)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41