DOM34/YNL001W Protein Information Help

Standard Name Dom34p 1
Systematic Name Ynl001wp
ORF Classification Verified
Description Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7, 8, 9)
Name Description Duplication Of Multilocus region 3
Experimental Data
Molecules/cell 1720 10
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 386
Molecular Weight (Da) 44,057
Isoelectric Point (pI) 5.49

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Dom34p (InterPro)
Physical Interactions There are 24 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000168400 | P33309
MIPS: YNL001W
NCBI: 115502382 | 1301812 | 166007233 | 166007234 | 166007235 | 295608 | 334359141 | 377656233 | 461949 | 496711 | 6324327 | NP_014397.1
GenBank/EMBL/DDBJ: DAA10542.1 | L11277 | X77114 | Z71277
External Classifications EC: 3.1.-.- [Hydrolases acting on ester bonds ]
Amino Acid Sequence (or in FASTA format)
 
       1  MKVISLKKDS FNKGGAVITL LPEDKEDLFT VYQIVDKDDE LIFKKKFTSK
      51  LDEAGKKKST DLVKLKIKVI SEDFDMKDEY LKYKGVTVTD ESGASNVDIP
     101  VGKYLSFTLD YVYPFTIIKQ NFNKFMQKLL NEACNIEYKS DTAAVVLQEG
     151  IAHVCLVTSS STILKQKIEY SMPKKKRTTD VLKFDEKTEK FYKAIYSAMK
     201  KDLNFDKLKT IILCSPGFYA KILMDKIFQY AEEEHNKKIL DNKGMFFIAH
     251  CSTGYLQGIN EVLKNPLYAS KLQDTKYSKE IMVMDEFLLH LNKDDDKAWY
     301  GEKEVVKAAE YGAISYLLLT DKVLHSDNIA QREEYLKLMD SVESNGGKAL
     351  VLSTLHSLGE ELDQLTGIAC ILKYPLPDLD EDDGEE*

external links for Dom34p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Dom34p
1) Lalo D, et al.  (1994) Organization of the centromeric region of chromosome XIV in Saccharomyces cerevisiae. Yeast 10(4):523-33
2) Ragan MA, et al.  (1996) An archaebacterial homolog of pelota, a meiotic cell division protein in eukaryotes. FEMS Microbiol Lett 144(2-3):151-5
3) Davis L and Engebrecht J  (1998) Yeast dom34 mutants are defective in multiple developmental pathways and exhibit decreased levels of polyribosomes. Genetics 149(1):45-56
4) Carr-Schmid A, et al.  (2002) Novel G-protein complex whose requirement is linked to the translational status of the cell. Mol Cell Biol 22(8):2564-74
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Lee HH, et al.  (2007) Structural and functional insights into Dom34, a key component of no-go mRNA decay. Mol Cell 27(6):938-50
7) Passos DO, et al.  (2009) Analysis of Dom34 and its function in no-go decay. Mol Biol Cell 20(13):3025-32
8) Shoemaker CJ, et al.  (2010) Dom34:Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay. Science 330(6002):369-72
9) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
10) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41