PRE5/YMR314W Protein Information

Standard Name	Pre5p
Systematic Name	Ymr314wp
ORF Classification	Verified
Description	Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress (1, 2)
Name Description	PRoteinase yscE

Experimental Data
Molecules/cell	7210 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	234
Molecular Weight (Da)	25,604
Isoelectric Point (pI)	7.43

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pre5p (InterPro)
Physical Interactions	There are 137 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052FDE \| P40302 MIPS: YMR314W NCBI: 112490847 \| 112490861 \| 11513417 \| 11513431 \| 11513996 \| 11514010 \| 14488810 \| 14488824 \| 178847464 \| 178847478 \| 178847506 \| 178847520 \| 190016371 \| 190016385 \| 197725325 \| 197725339 \| 20150381 \| 20150402 \| 211939216 \| 211939230 \| 211939244 \| 211939258 \| 238828241 \| 238828255 \| 258588325 \| 258588339 \| 258588353 \| 258588367 \| 258588547 \| 258588561 \| 298508227 \| 298508241 \| 298508443 \| 298508457 \| 3114274 \| 3114288 \| 323462940 \| 323462954 \| 323462968 \| 323462982 \| 323462996 \| 323463010 \| 330689385 \| 330689399 \| 333361148 \| 333361162 \| 333361176 \| 333361190 \| 333361204 \| 333361218 \| 333361232 \| 333361246 \| 335892345 \| 335892359 \| 340780423 \| 340780437 \| 340780451 \| 340780465 \| 350610676 \| 350610690 \| 359545850 \| 359545864 \| 378792245 \| 378792259 \| 378792279 \| 378792293 \| 390980820 \| 390980834 \| 390980848 \| 390980862 \| 402550736 \| 402550750 \| 403071966 \| 403072196 \| 409973864 \| 409973878 \| 409973892 \| 409973906 \| 444302343 \| 444302357 \| 444302371 \| 444302385 \| 444302399 \| 444302413 \| 45269848 \| 511903 \| 6323974 \| 730371 \| 75765704 \| 75765718 \| 93279372 \| 93279386 \| 984687 \| 99032206 \| 99032220 \| NP_014045.1 \| NM_001182825.1 GenBank/EMBL/DDBJ: DAA10215.1 \| AY557978 \| L34347 \| Z54141
External Classifications	EC: 3.4.25.1 [Proteasome endopeptidase complex]

Amino Acid Sequence (or in FASTA format)
1 MFRNNYDGDT VTFSPTGRLF QVEYALEAIK QGSVTVGLRS NTHAVLVALK 51 RNADELSSYQ KKIIKCDEHM GLSLAGLAPD ARVLSNYLRQ QCNYSSLVFN 101 RKLAVERAGH LLCDKAQKNT QSYGGRPYGV GLLIIGYDKS GAHLLEFQPS 151 GNVTELYGTA IGARSQGAKT YLERTLDTFI KIDGNPDELI KAGVEAISQS 201 LRDESLTVDN LSIAIVGKDT PFTIYDGEAV AKYI*

Amino Acid Sequence (or in FASTA format)

       1  MFRNNYDGDT VTFSPTGRLF QVEYALEAIK QGSVTVGLRS NTHAVLVALK
      51  RNADELSSYQ KKIIKCDEHM GLSLAGLAPD ARVLSNYLRQ QCNYSSLVFN
     101  RKLAVERAGH LLCDKAQKNT QSYGGRPYGV GLLIIGYDKS GAHLLEFQPS
     151  GNVTELYGTA IGARSQGAKT YLERTLDTFI KIDGNPDELI KAGVEAISQS
     201  LRDESLTVDN LSIAIVGKDT PFTIYDGEAV AKYI*

external links for Pre5p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Pre5p

1)	Heinemeyer W, et al. (1994) PRE5 and PRE6, the last missing genes encoding 20S proteasome subunits from yeast? Indication for a set of 14 different subunits in the eukaryotic proteasome core. Biochemistry 33(40):12229-37
2)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41