TGL3/YMR313C Protein Information Help

Standard Name Tgl3p
Systematic Name Ymr313cp
ORF Classification Verified
Description Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation (1, 2, 3)
Name Description TriacylGlycerol Lipase 1
Experimental Data
Molecules/cell 3210 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 642
Molecular Weight (Da) 73,612
Isoelectric Point (pI) 8.5

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Tgl3p (InterPro)
Transmembrane Domains There are 1 total predicted transmembrane domains (TMHMM)
Signal Peptide(s) There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions There are 11 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013B94F | P40308
MIPS: YMR313C
NCBI: 1730604 | 553138 | 6323973 | 984686 | NP_014044.1
GenBank/EMBL/DDBJ: DAA10214.1 | L34347 | Z54141
External Classifications EC: 3.1.1.3 [Triacylglycerol lipase]
Amino Acid Sequence (or in FASTA format)
 
       1  MKETAQEYKV SAVIPTLLKN WILRVVYATL DHIPPFVWEI LHVITDIYFF
      51  WVQKLINYVR PHSRVIYYNA IKKLDECDTY QMWCQQASVV DEITGANLWR
     101  RNFFSRRYDF NSVIEQYSIL ENMLREEKYD VVKEKFSTTG PCMLRNFAGI
     151  GDKKLFTKSL MGTKLLIEQY LTRILEGLDI LNNQTLTPTS FFQRCKLSLG
     201  TTALILQGGS LFGLFHLGVI RGLLLQDLMP NIISGSSMGA CVASLFGCLS
     251  NEQLKQLLTD DNLLNIIKND VDLLKSCGYG NLEQHLNLGT LIQNLIHHGY
     301  SQDVYLFIRF VMKYIVKEKT FEEVYQITGK VFNIVIHPTD KSCPNLLNYV
     351  TTPNVLIKSA IECSLGSGVI SEDTSLLCKN LENEIEPFLN INKNKQVKFL
     401  TPENANNPSI TESPYTRLTE LFNVNNFIVS LARPYLAPLV VNDLKHEIKT
     451  SKYYYYKHYP NMPPINANTV RKTQRSSSQS PIKAGTVEDL EPEPLMSPVP
     501  PSSAVNDSAE YIIPELGIPQ LNFTEMEPLA FKFKYHLERK LKNIATMEFR
     551  HRMEVLDNLG LLCSLIKRLI IDEKTPRSAT EIAVVPRMKS LSLTRIIEGQ
     601  LNNIPYWIKS GERSTWPALA LIKTRCAVEF KLDDIIRARR SR*

external links for Tgl3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Tgl3p
1) Athenstaedt K and Daum G  (2003) YMR313c/TGL3 encodes a novel triacylglycerol lipase located in lipid particles of Saccharomyces cerevisiae. J Biol Chem 278(26):23317-23
2) Kurat CF, et al.  (2009) Cdk1/Cdc28-dependent activation of the major triacylglycerol lipase Tgl4 in yeast links lipolysis to cell-cycle progression. Mol Cell 33(1):53-63
3) Rajakumari S and Daum G  (2010) Janus-faced Enzymes Yeast Tgl3p and Tgl5p Catalyze Lipase and Acyltransferase Reactions. Mol Biol Cell 21(4):501-10
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41