PSE1/YMR308C Protein Information

Standard Name	Pse1p
Systematic Name	Ymr308cp
Alias	Kap121p
ORF Classification	Verified
Description	Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p (1, 2, 3, 4, 5, 6)
Name Description	Protein Secretion Enhancer

Experimental Data
Molecules/cell	15500 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,089
Molecular Weight (Da)	121,029
Isoelectric Point (pI)	4.45

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pse1p (InterPro)
Physical Interactions	There are 219 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052EC1 \| P32337 MIPS: YMR308C NCBI: 1709850 \| 255385 \| 4246 \| 6323968 \| 798950 \| NP_014039.1 GenBank/EMBL/DDBJ: DAA10209.1 \| S45357 \| Z11538 \| Z49212

Amino Acid Sequence (or in FASTA format)
1 MSALPEEVNR TLLQIVQAFA SPDNQIRSVA EKALSEEWIT ENNIEYLLTF 51 LAEQAAFSQD TTVAALSAVL FRKLALKAPP SSKLMIMSKN ITHIRKEVLA 101 QIRSSLLKGF LSERADSIRH KLSDAIAECV QDDLPAWPEL LQALIESLKS 151 GNPNFRESSF RILTTVPYLI TAVDINSILP IFQSGFTDAS DNVKIAAVTA 201 FVGYFKQLPK SEWSKLGILL PSLLNSLPRF LDDGKDDALA SVFESLIELV 251 ELAPKLFKDM FDQIIQFTDM VIKNKDLEPP ARTTALELLT VFSENAPQMC 301 KSNQNYGQTL VMVTLIMMTE VSIDDDDAAE WIESDDTDDE EEVTYDHARQ 351 ALDRVALKLG GEYLAAPLFQ YLQQMITSTE WRERFAAMMA LSSAAEGCAD 401 VLIGEIPKIL DMVIPLINDP HPRVQYGCCN VLGQISTDFS PFIQRTAHDR 451 ILPALISKLT SECTSRVQTH AAAALVNFSE FASKDILEPY LDSLLTNLLV 501 LLQSNKLYVQ EQALTTIAFI AEAAKNKFIK YYDTLMPLLL NVLKVNNKDN 551 SVLKGKCMEC ATLIGFAVGK EKFHEHSQEL ISILVALQNS DIDEDDALRS 601 YLEQSWSRIC RILGDDFVPL LPIVIPPLLI TAKATQDVGL IEEEEAANFQ 651 QYPDWDVVQV QGKHIAIHTS VLDDKVSAME LLQSYATLLR GQFAVYVKEV 701 MEEIALPSLD FYLHDGVRAA GATLIPILLS CLLAATGTQN EELVLLWHKA 751 SSKLIGGLMS EPMPEITQVY HNSLVNGIKV MGDNCLSEDQ LAAFTKGVSA 801 NLTDTYERMQ DRHGDGDEYN ENIDEEEDFT DEDLLDEINK SIAAVLKTTN 851 GHYLKNLENI WPMINTFLLD NEPILVIFAL VVIGDLIQYG GEQTASMKNA 901 FIPKVTECLI SPDARIRQAA SYIIGVCAQY APSTYADVCI PTLDTLVQIV 951 DFPGSKLEEN RSSTENASAA IAKILYAYNS NIPNVDTYTA NWFKTLPTIT 1001 DKEAASFNYQ FLSQLIENNS PIVCAQSNIS AVVDSVIQAL NERSLTEREG 1051 QTVISSVKKL LGFLPSSDAM AIFNRYPADI MEKVHKWFA*

Amino Acid Sequence (or in FASTA format)

       1  MSALPEEVNR TLLQIVQAFA SPDNQIRSVA EKALSEEWIT ENNIEYLLTF
      51  LAEQAAFSQD TTVAALSAVL FRKLALKAPP SSKLMIMSKN ITHIRKEVLA
     101  QIRSSLLKGF LSERADSIRH KLSDAIAECV QDDLPAWPEL LQALIESLKS
     151  GNPNFRESSF RILTTVPYLI TAVDINSILP IFQSGFTDAS DNVKIAAVTA
     201  FVGYFKQLPK SEWSKLGILL PSLLNSLPRF LDDGKDDALA SVFESLIELV
     251  ELAPKLFKDM FDQIIQFTDM VIKNKDLEPP ARTTALELLT VFSENAPQMC
     301  KSNQNYGQTL VMVTLIMMTE VSIDDDDAAE WIESDDTDDE EEVTYDHARQ
     351  ALDRVALKLG GEYLAAPLFQ YLQQMITSTE WRERFAAMMA LSSAAEGCAD
     401  VLIGEIPKIL DMVIPLINDP HPRVQYGCCN VLGQISTDFS PFIQRTAHDR
     451  ILPALISKLT SECTSRVQTH AAAALVNFSE FASKDILEPY LDSLLTNLLV
     501  LLQSNKLYVQ EQALTTIAFI AEAAKNKFIK YYDTLMPLLL NVLKVNNKDN
     551  SVLKGKCMEC ATLIGFAVGK EKFHEHSQEL ISILVALQNS DIDEDDALRS
     601  YLEQSWSRIC RILGDDFVPL LPIVIPPLLI TAKATQDVGL IEEEEAANFQ
     651  QYPDWDVVQV QGKHIAIHTS VLDDKVSAME LLQSYATLLR GQFAVYVKEV
     701  MEEIALPSLD FYLHDGVRAA GATLIPILLS CLLAATGTQN EELVLLWHKA
     751  SSKLIGGLMS EPMPEITQVY HNSLVNGIKV MGDNCLSEDQ LAAFTKGVSA
     801  NLTDTYERMQ DRHGDGDEYN ENIDEEEDFT DEDLLDEINK SIAAVLKTTN
     851  GHYLKNLENI WPMINTFLLD NEPILVIFAL VVIGDLIQYG GEQTASMKNA
     901  FIPKVTECLI SPDARIRQAA SYIIGVCAQY APSTYADVCI PTLDTLVQIV
     951  DFPGSKLEEN RSSTENASAA IAKILYAYNS NIPNVDTYTA NWFKTLPTIT
    1001  DKEAASFNYQ FLSQLIENNS PIVCAQSNIS AVVDSVIQAL NERSLTEREG
    1051  QTVISSVKKL LGFLPSSDAM AIFNRYPADI MEKVHKWFA*

external links for Pse1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Pse1p

1)	Seedorf M and Silver PA (1997) Importin/karyopherin protein family members required for mRNA export from the nucleus. Proc Natl Acad Sci U S A 94(16):8590-5
2)	Marelli M, et al. (1998) Specific binding of the karyopherin Kap121p to a subunit of the nuclear pore complex containing Nup53p, Nup59p, and Nup170p. J Cell Biol 143(7):1813-30
3)	Isoyama T, et al. (2001) Nuclear import of the yeast AP-1-like transcription factor Yap1p is mediated by transport receptor Pse1p, and this import step is not affected by oxidative stress. J Biol Chem 276(24):21863-9
4)	Leslie DM, et al. (2002) Kap121p-mediated nuclear import is required for mating and cellular differentiation in yeast. Mol Cell Biol 22(8):2544-55
5)	Delahodde A, et al. (2001) Pse1/Kap121-dependent nuclear localization of the major yeast multidrug resistance (MDR) transcription factor Pdr1. Mol Microbiol 39(2):304-12
6)	Ueta R, et al. (2003) Pse1p mediates the nuclear import of the iron-responsive transcription factor Aft1p in Saccharomyces cerevisiae. J Biol Chem 278(50):50120-7
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41