TDA1/YMR291W Protein Information Help

Standard Name Tda1p 1
Systematic Name Ymr291wp
ORF Classification Verified
Description Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress (1, 2, 3, 4, 5, 6)
Name Description Topoisomerase I Damage Affected 1
Experimental Data
Molecules/cell 10200 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 586
Molecular Weight (Da) 66,219
Isoelectric Point (pI) 6.94

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Tda1p (InterPro)
Physical Interactions There are 30 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012DEFA | Q03533
MIPS: YMR291W
NCBI: 2499620 | 530348 | 6323948 | NP_014019.1
GenBank/EMBL/DDBJ: DAA10192.1 | X80836
External Classifications EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MTTASSSASQ LQQRLPEEKP WPQLSGSNAD AQTFKCKYVT NHNSLGDGNF
      51  SVVKECMNIH TKDLYAMKLI KKQTVKNKIQ LIQREFDLLR SISEKIRDME
     101  KKNEHSLDIF EGHHHILQLF DYFETADNIV LITQLCQKGD LYEKIVENQC
     151  LDLETQVTSY CACLVSVLEF LHSQGIVHRD LKAENVLFRL RVNENEKNLQ
     201  GEHHGDFKYD LLAHDLVLAD FGLAAEYNTS KVNSLKEFVG TISYIAPEIV
     251  KCKGVGEMTP DQVGKLDKYG CPVDIWALGV LTYFMAFGYT PFDCTTDDET
     301  LECISKCDYY VDEQMMHDPK YEQFWNFVQC CFTIDPAVRR SAKNLKQHPF
     351  IKDYFATSNS LNTKDTPNFS FHPTIRRVSS TASMHTLRSP SKSRKTTTLA
     401  YLNMDGGSSE TSTAFSSKMD LPDLYVDRTI NSRERSLNRI RDTLKKTLSM
     451  TSLKPAGTFD YLHANKNGTS LSSSKSGLVK KNSTFVLDPK PPKNSLMNGC
     501  FSTTPESRSN FNTPKTLSRQ GSSTSVKKYV NEVDLLLTPR TASMSSNDTT
     551  AINDYDTTND KNPARKHAAS FQVNVDDSDG DETMQI*

external links for Tda1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Tda1p
1) Reid RJ, et al.  (2011) Selective ploidy ablation, a high-throughput plasmid transfer protocol, identifies new genes affecting topoisomerase I-induced DNA damage. Genome Res 21(3):477-86
2) Hunter T and Plowman GD  (1997) The protein kinases of budding yeast: six score and more. Trends Biochem Sci 22(1):18-22
3) Giaever G, et al.  (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91
4) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
5) Ptacek J, et al.  (2005) Global analysis of protein phosphorylation in yeast. Nature 438(7068):679-84
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41