YKU70/YMR284W Protein Information

Standard Name	Yku70p 1
Systematic Name	Ymr284wp
Alias	Hdf1p 2 , Nes24p 3
ORF Classification	Verified
Description	Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair (1, 10, 4, 5, 6, 7, 8, 9) Also known as: KU70
Name Description	Yeast KU protein 1

Experimental Data
Molecules/cell	892 11

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	602
Molecular Weight (Da)	70,647
Isoelectric Point (pI)	6.31

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Yku70p (InterPro)
Physical Interactions	There are 46 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000004C4D7 \| P32807 MIPS: YMR284W NCBI: 112362573 \| 112362575 \| 112362577 \| 112362579 \| 112362581 \| 112362583 \| 112362585 \| 112362587 \| 112362591 \| 112362593 \| 112362595 \| 112362597 \| 1346259 \| 287606 \| 3765 \| 51013177 \| 6323940 \| 825550 \| NP_014011.1 GenBank/EMBL/DDBJ: DAA10185.1 \| AM296292 \| AM296293 \| AM296294 \| AM296295 \| AM296296 \| AM296297 \| AM296298 \| AM296299 \| AM296301 \| AM296302 \| AM296303 \| AM296304 \| AY692863 \| D15052 \| X70379 \| Z49704
External Classifications	EC: 3.6.4.12 [DNA helicase]

Amino Acid Sequence (or in FASTA format)
1 MRSVTNAFGN SGELNDQVDE TGYRKFDIHE GILFCIELSE TMFKESSDLE 51 YKSPLLEILE SLDELMSQLV ITRPGTAIGC YFYYCNREDA KEGIYELFPL 101 RDINATFMKK LNDLLEDLSS GRISLYDYFM FQQTGSEKQV RLSVLFTFML 151 DTFLEEIPGQ KQLSNKRVFL FTDIDKPQEA QDIDERARLR RLTIDLFDNK 201 VNFATFFIGY ADKPFDNEFY SDILQLGSHT NENTGLDSEF DGPSTKPIDA 251 KYIKSRILRK KEVKRIMFQC PLILDEKTNF IVGVKGYTMY THEKAGVRYK 301 LVYEHEDIRQ EAYSKRKFLN PITGEDVTGK TVKVYPYGDL DINLSDSQDQ 351 IVMEAYTQKD AFLKIIGFRS SSKSIHYFNN IDKSSFIVPD EAKYEGSIRT 401 LASLLKILRK KDKIAILWGK LKSNSHPSLY TLSPSSVKDY NEGFYLYRVP 451 FLDEIRKFPS LLSYDDGSEH KLDYDNMKKV TQSIMGYFNL RDGYNPSDFK 501 NPLLQKHYKV LHDYLLQIET TFDENETPNT KKDRMMREDD SLRKLYYIRN 551 KILESEKSED PIIQRLNKYV KIWNMFYKKF NDDNISIKEE KKPFDKKPKF 601 NI*

Amino Acid Sequence (or in FASTA format)

       1  MRSVTNAFGN SGELNDQVDE TGYRKFDIHE GILFCIELSE TMFKESSDLE
      51  YKSPLLEILE SLDELMSQLV ITRPGTAIGC YFYYCNREDA KEGIYELFPL
     101  RDINATFMKK LNDLLEDLSS GRISLYDYFM FQQTGSEKQV RLSVLFTFML
     151  DTFLEEIPGQ KQLSNKRVFL FTDIDKPQEA QDIDERARLR RLTIDLFDNK
     201  VNFATFFIGY ADKPFDNEFY SDILQLGSHT NENTGLDSEF DGPSTKPIDA
     251  KYIKSRILRK KEVKRIMFQC PLILDEKTNF IVGVKGYTMY THEKAGVRYK
     301  LVYEHEDIRQ EAYSKRKFLN PITGEDVTGK TVKVYPYGDL DINLSDSQDQ
     351  IVMEAYTQKD AFLKIIGFRS SSKSIHYFNN IDKSSFIVPD EAKYEGSIRT
     401  LASLLKILRK KDKIAILWGK LKSNSHPSLY TLSPSSVKDY NEGFYLYRVP
     451  FLDEIRKFPS LLSYDDGSEH KLDYDNMKKV TQSIMGYFNL RDGYNPSDFK
     501  NPLLQKHYKV LHDYLLQIET TFDENETPNT KKDRMMREDD SLRKLYYIRN
     551  KILESEKSED PIIQRLNKYV KIWNMFYKKF NDDNISIKEE KKPFDKKPKF
     601  NI*

external links for Yku70p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Yku70p

1)	Boulton SJ and Jackson SP (1996) Saccharomyces cerevisiae Ku70 potentiates illegitimate DNA double-strand break repair and serves as a barrier to error-prone DNA repair pathways. EMBO J 15(18):5093-103
2)	Feldmann H and Winnacker EL (1993) A putative homologue of the human autoantigen Ku from Saccharomyces cerevisiae. J Biol Chem 268(17):12895-900
3)	Yoshida M, et al. (1994) Cloning and sequencing of the NES24 gene of Saccharomyces cerevisiae. Yeast 10(3):371-6
4)	Martin SG, et al. (1999) Relocalization of telomeric Ku and SIR proteins in response to DNA strand breaks in yeast. Cell 97(5):621-33
5)	Milne GT, et al. (1996) Mutations in two Ku homologs define a DNA end-joining repair pathway in Saccharomyces cerevisiae. Mol Cell Biol 16(8):4189-98
6)	Mages GJ, et al. (1996) Involvement of the Saccharomyces cerevisiae HDF1 gene in DNA double-strand break repair and recombination. J Biol Chem 271(14):7910-5
7)	Boulton SJ and Jackson SP (1998) Components of the Ku-dependent non-homologous end-joining pathway are involved in telomeric length maintenance and telomeric silencing. EMBO J 17(6):1819-28
8)	Porter SE, et al. (1996) The DNA-binding protein Hdf1p (a putative Ku homologue) is required for maintaining normal telomere length in Saccharomyces cerevisiae. Nucleic Acids Res 24(4):582-5
9)	Laroche T, et al. (1998) Mutation of yeast Ku genes disrupts the subnuclear organization of telomeres. Curr Biol 8(11):653-6
10)	Bertuch AA and Lundblad V (2003) The Ku heterodimer performs separable activities at double-strand breaks and chromosome termini. Mol Cell Biol 23(22):8202-15
11)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41