ZDS1/YMR273C Protein Information

Standard Name	Zds1p 1, 2
Systematic Name	Ymr273cp
Alias	Ces1p 3 , Ckm1p , Nrc1p , Oss1p 4
ORF Classification	Verified
Description	Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication (10, 11, 2, 5, 6, 7, 8, 9)
Name Description	Zillion Different Screens 1, 2

Experimental Data
Molecules/cell	279 12

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	915
Molecular Weight (Da)	103,358
Isoelectric Point (pI)	6.32

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Zds1p (InterPro)
Physical Interactions	There are 64 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000004F95C \| P50111 MIPS: YMR273C NCBI: 1498490 \| 1508861 \| 1709345 \| 1816450 \| 6323929 \| 809096 \| 857372 \| 929925 \| NP_014000.1 GenBank/EMBL/DDBJ: DAA10174.1 \| D50276 \| L42821 \| U32580 \| U63849 \| U65682 \| Z49260

Amino Acid Sequence (or in FASTA format)
1 MSNRDNESML RTTSSDKAIA SQRDKRKSEV LIAAQSLDNE IRSVKNLKRL 51 SIGSMDLLID PELDIKFGGE SSGRRSWSGT TSSSASMPSD TTTVNNTRYS 101 DPTPLENLHG RGNSGIESSN KTKQGNYLGI KKGVHSPSRK LNANVLKKNL 151 LWVPANQHPN VKPDNFLELV QDTLQNIQLS DNGEDNDGNS NENNDIEDNG 201 EDKESQSYEN KENNTINLNR GLSRHGNASL IRRPSTLRRS YTEFDDNEDD 251 DNKGDSASET VNKVEERISK IKERPVSLRD ITEELTKISN SAGLTDNDAI 301 TLARTLSMAG SYSDKKDQPQ PEGHYDEGDI GFSTSQANTL DDGEFASNMP 351 INNTMTWPER SSLRRSRFNT YRIRSQEQEK EVEQSVDEMK NDDEERLKLT 401 KNTIKVEIDP HKSPFRQQDE DSENMSSPGS IGDFQDIYNH YRQSSGEWEQ 451 EMGIEKEAEE VPVKVRNDTV EQDLELREGT TDMVKPSATD DNKETKRHRR 501 RNGWTWLNNK MSREDDNEEN QGDDENEENV DSQRMELDNS KKHYISLFNG 551 GEKTEVSNKE EMNNSSTSTA TSQTRQKIEK TFANLFRRKP HHKHDASSSP 601 SSSPSSSPSI PNNDAVHVRV RKSKKLGNKS GREPVEPIVL RNRPRPHRHH 651 HSRHGSQKIS VKTLKDSQPQ QQIPLQPQLE GAIEIEKKEE SDSESLPQLQ 701 PAVSVSSTKS NSRDREEEEA KKKNKKRSNT TEISNQQHSK HVQKENTDEQ 751 KAQLQAPAQE QVQTSVPVQA SAPVQNSAPV QTSAPVEASA QTQAPAAPPL 801 KHTSILPPRK LTFADVKKPD KPNSPVQFTD SAFGFPLPLL TVSTVIMFDH 851 RLPINVERAI YRLSHLKLSN SKRGLREQVL LSNFMYAYLN LVNHTLYMEQ 901 VAHDKEQQQQ QQQQP*

Amino Acid Sequence (or in FASTA format)

       1  MSNRDNESML RTTSSDKAIA SQRDKRKSEV LIAAQSLDNE IRSVKNLKRL
      51  SIGSMDLLID PELDIKFGGE SSGRRSWSGT TSSSASMPSD TTTVNNTRYS
     101  DPTPLENLHG RGNSGIESSN KTKQGNYLGI KKGVHSPSRK LNANVLKKNL
     151  LWVPANQHPN VKPDNFLELV QDTLQNIQLS DNGEDNDGNS NENNDIEDNG
     201  EDKESQSYEN KENNTINLNR GLSRHGNASL IRRPSTLRRS YTEFDDNEDD
     251  DNKGDSASET VNKVEERISK IKERPVSLRD ITEELTKISN SAGLTDNDAI
     301  TLARTLSMAG SYSDKKDQPQ PEGHYDEGDI GFSTSQANTL DDGEFASNMP
     351  INNTMTWPER SSLRRSRFNT YRIRSQEQEK EVEQSVDEMK NDDEERLKLT
     401  KNTIKVEIDP HKSPFRQQDE DSENMSSPGS IGDFQDIYNH YRQSSGEWEQ
     451  EMGIEKEAEE VPVKVRNDTV EQDLELREGT TDMVKPSATD DNKETKRHRR
     501  RNGWTWLNNK MSREDDNEEN QGDDENEENV DSQRMELDNS KKHYISLFNG
     551  GEKTEVSNKE EMNNSSTSTA TSQTRQKIEK TFANLFRRKP HHKHDASSSP
     601  SSSPSSSPSI PNNDAVHVRV RKSKKLGNKS GREPVEPIVL RNRPRPHRHH
     651  HSRHGSQKIS VKTLKDSQPQ QQIPLQPQLE GAIEIEKKEE SDSESLPQLQ
     701  PAVSVSSTKS NSRDREEEEA KKKNKKRSNT TEISNQQHSK HVQKENTDEQ
     751  KAQLQAPAQE QVQTSVPVQA SAPVQNSAPV QTSAPVEASA QTQAPAAPPL
     801  KHTSILPPRK LTFADVKKPD KPNSPVQFTD SAFGFPLPLL TVSTVIMFDH
     851  RLPINVERAI YRLSHLKLSN SKRGLREQVL LSNFMYAYLN LVNHTLYMEQ
     901  VAHDKEQQQQ QQQQP*

external links for Zds1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Zds1p

1)	Yu Y, et al. (1996) Mutations in the homologous ZDS1 and ZDS2 genes affect cell cycle progression. Mol Cell Biol 16(10):5254-63
2)	Bi E and Pringle JR (1996) ZDS1 and ZDS2, genes whose products may regulate Cdc42p in Saccharomyces cerevisiae. Mol Cell Biol 16(10):5264-75
3)	Schwer B and Shuman S (1996) Multicopy suppressors of temperature-sensitive mutations of yeast mRNA capping enzyme. Gene Expr 5(6):331-44
4)	Ma XJ, et al. (1996) A search for proteins that interact genetically with histone H3 and H4 amino termini uncovers novel regulators of the Swe1 kinase in Saccharomyces cerevisiae. Genes Dev 10(11):1327-40
5)	Roy N and Runge KW (1999) The ZDS1 and ZDS2 proteins require the Sir3p component of yeast silent chromatin to enhance the stability of short linear centromeric plasmids. Chromosoma 108(3):146-61
6)	Griffioen G, et al. (2001) Nucleocytoplasmic distribution of budding yeast protein kinase A regulatory subunit Bcy1 requires Zds1 and is regulated by Yak1-dependent phosphorylation of its targeting domain. Mol Cell Biol 21(2):511-23
7)	Estruch F, et al. (2005) Physical and genetic interactions link the yeast protein Zds1p with mRNA nuclear export. J Biol Chem 280(10):9691-7
8)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
9)	Queralt E and Uhlmann F (2008) Separase cooperates with Zds1 and Zds2 to activate Cdc14 phosphatase in early anaphase. J Cell Biol 182(5):873-83
10)	Yasutis K, et al. (2010) Zds2p Regulates Swe1p-dependent Polarized Cell Growth in Saccharomyces cerevisiae via a Novel Cdc55p Interaction Domain. Mol Biol Cell 21(24):4373-86
11)	Rossio V and Yoshida S (2011) Spatial regulation of Cdc55-PP2A by Zds1/Zds2 controls mitotic entry and mitotic exit in budding yeast. J Cell Biol 193(3):445-54
12)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41