CUE1/YMR264W Protein Information

Standard Name	Cue1p 1
Systematic Name	Ymr264wp
Alias	Kis4p 2
ORF Classification	Verified
Description	Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication (1, 3, 4)
Name Description	Coupling of Ubiquitin conjugation to ER degradation 1

Experimental Data
Molecules/cell	589 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	203
Molecular Weight (Da)	22,762
Isoelectric Point (pI)	6.69

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Cue1p (InterPro)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 33 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013B93C \| P38428 MIPS: YMR264W NCBI: 173195 \| 2506768 \| 45269838 \| 6323920 \| 809087 \| NP_013991.1 GenBank/EMBL/DDBJ: DAA10164.1 \| AY557973 \| M88172 \| Z49260

Amino Acid Sequence (or in FASTA format)
1 MEDSRLLITL ILVFGVIFLK KFFQSNQHPS AQRLSATGVN AHGRPQGSTQ 51 NALRRTGRVN GGHPVTTQMV ETVQNLAPNL HPEQIRYSLE NTGSVEETVE 101 RYLRGDEFSF PPGFEPSRAP MGANAAVDNN AAGGGEFNDP RKKNMICAEN 151 LLDKFHVDLN EDMSNLSFKD LDIEERKRLL VWQARKNLET KLQSDKDLQS 201 LLT*

Amino Acid Sequence (or in FASTA format)

       1  MEDSRLLITL ILVFGVIFLK KFFQSNQHPS AQRLSATGVN AHGRPQGSTQ
      51  NALRRTGRVN GGHPVTTQMV ETVQNLAPNL HPEQIRYSLE NTGSVEETVE
     101  RYLRGDEFSF PPGFEPSRAP MGANAAVDNN AAGGGEFNDP RKKNMICAEN
     151  LLDKFHVDLN EDMSNLSFKD LDIEERKRLL VWQARKNLET KLQSDKDLQS
     201  LLT*

external links for Cue1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Cue1p

1)	Biederer T, et al. (1997) Role of Cue1p in ubiquitination and degradation at the ER surface. Science 278(5344):1806-9
2)	Kopski KM and Huffaker TC (1997) Suppressors of the ndc10-2 mutation: a role for the ubiquitin system in Saccharomyces cerevisiae kinetochore function. Genetics 147(2):409-20
3)	Shih SC, et al. (2003) A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain. EMBO J 22(6):1273-81
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41