RKR1/YMR247C Protein Information

Standard Name	Rkr1p 1
Systematic Name	Ymr247cp
Alias	Ltn1p 2
ORF Classification	Verified
Description	RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice (1, 2, 3)
Name Description	RING domain mutant Killed by rtf1 deletion 1

Experimental Data
Molecules/cell	222 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,562
Molecular Weight (Da)	180,184
Isoelectric Point (pI)	5.24

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rkr1p (InterPro)
Physical Interactions	There are 6 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013B92C \| Q04781 MIPS: YMR247C NCBI: 2497203 \| 6323904 \| 732925 \| 736313 \| NP_013975.1 GenBank/EMBL/DDBJ: DAA10148.1 \| Z48639 \| Z48756

Amino Acid Sequence (or in FASTA format)
1 MSFGGINTFQ QYNTDLGLGH NGVRISLNYF DGLPDPSLLN SLYSNELKLI 51 FKSLLKRDET TKEKALMDLS NLISDFNQNE YFFNDIFLLC WSQIYAKLII 101 SDYKVIRLQS HQITIMLVKS LRKKISKFLK DFIPLILLGT CELDYSVSKP 151 SLNELTECFN KDPAKINALW AVFQEQLLNL VKEIVVNENE DTISDERYSS 201 KEESEFRYHR VIASAVLLLI KLFVHNKDVS ERNSSSLKVI LSDESIWKLL 251 NLKNGQNTNA YETVLRLIDV LYTRGYMPSH KNIMKLAVKK LLKSLTHITS 301 KNILKVCPVL PSILNLLATL DDYEDGTIWS YDKSSKEKVL KFLSVSRTSP 351 SPGFFNAVFA LYSSTKRHSF LDYYLEWLPF WQKSVQRLNE KGFSARNSAE 401 VLNEFWTNFL KFAEDSSEER VKKMVESEIF NSLSCGKSLS EYTKLNQTLS 451 GVFPPDKWER EIEDYFTSDE DIRKIKVSFE KNLFALLVTS PNNESAISRL 501 FDFFVQLIET DPSNVFNKYD GVYDALNYFL DSDMIFLNGK IGKFINEIPT 551 LVQESTYQNF AGIMAQYSNS KFFKMNTDAI TSLEDFFIVA LSFNLPKTII 601 LATMNELDND IYQQLMKSDS LELELYIEDF MKNYKFDDSG EIFKGNNKFL 651 NQRTITTLYR SAVANGQVEQ FCAVLSKLDE TFFSTLLLNT DFLSCALYEV 701 SEDTNEKLFK LSLQLAKGNS EIANKLAQVI LQHAQVYFSP GAKEKYVTHA 751 VELINGCNDT SQIFFPANAI EVFARYMPAI DYRSSLVSSL STNTHLLLTD 801 DKPINLKNMQ KLIRYALFLD ALLDALPERV NNHIVAFITV VSELVTDYNC 851 LSEEPNDLYY DFGHTFFKHG KVNLNFSDIV GNVIQPANGG DAMLTFDIAE 901 SNSVYFFYYS RVLYKVLLNS IDTVSSTTLN GLLASVESFV TKTVRDQKST 951 DKDYLLCAIL LLMFNRSNSK DEITKLRTLL ASQLIGIREV ELVDQEFKSL 1001 ALLNNLLDIP QADKQFVPIA PQRLNMIFRS ILKWLDSDLA YEPSFSTVRL 1051 LLLDFFTKLM RFEGVRDMGI TAFELSERLL ADSLSMCQID DTLYLLELRS 1101 SCLNLYETLS QGVSKNGEEI SEYGDEIQEN LIELMFLNFN QERNNQVSTL 1151 FYQKLYKVIS SMELKKLESQ YKRIFEVVLN DKDIGSNINQ SRLLTTLLGS 1201 LVVKTQQDII IEYELRIQKQ TGSDVDGSAS DNDVNSKFKL PQKLLQKVTD 1251 EVPKEYLEYE NKNSFIKYLW YWHLILMYFK DTSYNMRQIF IEQLKEAGLI 1301 NRMFDFITDQ IDLRDTEFWK QVDTKEISEY NIVGNNFSPY KEDIFEECKK 1351 LLGHTLYQLF NNVGCLTSIW WLNIKDRTLQ NDIEKFVSEF ISPILIKNEF 1401 DDINSKMDRL TSNDDALTIK LNNITNEVKA SYLIDDQKLE ISFKLPKNYP 1451 LTNIQVNGVS RVGISEQKWK QWIMSTQHVI TGMNGSVLDS LELFTKNVHL 1501 QFSGFEECAI CYSILHAVDR KLPSKTCPTC KNKFHGACLY KWFRSSGNNT 1551 CPLCRSEIPF RR*

Amino Acid Sequence (or in FASTA format)

       1  MSFGGINTFQ QYNTDLGLGH NGVRISLNYF DGLPDPSLLN SLYSNELKLI
      51  FKSLLKRDET TKEKALMDLS NLISDFNQNE YFFNDIFLLC WSQIYAKLII
     101  SDYKVIRLQS HQITIMLVKS LRKKISKFLK DFIPLILLGT CELDYSVSKP
     151  SLNELTECFN KDPAKINALW AVFQEQLLNL VKEIVVNENE DTISDERYSS
     201  KEESEFRYHR VIASAVLLLI KLFVHNKDVS ERNSSSLKVI LSDESIWKLL
     251  NLKNGQNTNA YETVLRLIDV LYTRGYMPSH KNIMKLAVKK LLKSLTHITS
     301  KNILKVCPVL PSILNLLATL DDYEDGTIWS YDKSSKEKVL KFLSVSRTSP
     351  SPGFFNAVFA LYSSTKRHSF LDYYLEWLPF WQKSVQRLNE KGFSARNSAE
     401  VLNEFWTNFL KFAEDSSEER VKKMVESEIF NSLSCGKSLS EYTKLNQTLS
     451  GVFPPDKWER EIEDYFTSDE DIRKIKVSFE KNLFALLVTS PNNESAISRL
     501  FDFFVQLIET DPSNVFNKYD GVYDALNYFL DSDMIFLNGK IGKFINEIPT
     551  LVQESTYQNF AGIMAQYSNS KFFKMNTDAI TSLEDFFIVA LSFNLPKTII
     601  LATMNELDND IYQQLMKSDS LELELYIEDF MKNYKFDDSG EIFKGNNKFL
     651  NQRTITTLYR SAVANGQVEQ FCAVLSKLDE TFFSTLLLNT DFLSCALYEV
     701  SEDTNEKLFK LSLQLAKGNS EIANKLAQVI LQHAQVYFSP GAKEKYVTHA
     751  VELINGCNDT SQIFFPANAI EVFARYMPAI DYRSSLVSSL STNTHLLLTD
     801  DKPINLKNMQ KLIRYALFLD ALLDALPERV NNHIVAFITV VSELVTDYNC
     851  LSEEPNDLYY DFGHTFFKHG KVNLNFSDIV GNVIQPANGG DAMLTFDIAE
     901  SNSVYFFYYS RVLYKVLLNS IDTVSSTTLN GLLASVESFV TKTVRDQKST
     951  DKDYLLCAIL LLMFNRSNSK DEITKLRTLL ASQLIGIREV ELVDQEFKSL
    1001  ALLNNLLDIP QADKQFVPIA PQRLNMIFRS ILKWLDSDLA YEPSFSTVRL
    1051  LLLDFFTKLM RFEGVRDMGI TAFELSERLL ADSLSMCQID DTLYLLELRS
    1101  SCLNLYETLS QGVSKNGEEI SEYGDEIQEN LIELMFLNFN QERNNQVSTL
    1151  FYQKLYKVIS SMELKKLESQ YKRIFEVVLN DKDIGSNINQ SRLLTTLLGS
    1201  LVVKTQQDII IEYELRIQKQ TGSDVDGSAS DNDVNSKFKL PQKLLQKVTD
    1251  EVPKEYLEYE NKNSFIKYLW YWHLILMYFK DTSYNMRQIF IEQLKEAGLI
    1301  NRMFDFITDQ IDLRDTEFWK QVDTKEISEY NIVGNNFSPY KEDIFEECKK
    1351  LLGHTLYQLF NNVGCLTSIW WLNIKDRTLQ NDIEKFVSEF ISPILIKNEF
    1401  DDINSKMDRL TSNDDALTIK LNNITNEVKA SYLIDDQKLE ISFKLPKNYP
    1451  LTNIQVNGVS RVGISEQKWK QWIMSTQHVI TGMNGSVLDS LELFTKNVHL
    1501  QFSGFEECAI CYSILHAVDR KLPSKTCPTC KNKFHGACLY KWFRSSGNNT
    1551  CPLCRSEIPF RR*

external links for Rkr1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS
YGOB	CYC2008 (complexes)	Pfam domains
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	GeneMANIA

References cited on this page View Complete Literature Guide for Rkr1p

1)	Braun MA, et al. (2007) Identification of Rkr1, a nuclear RING domain protein with functional connections to chromatin modification in Saccharomyces cerevisiae. Mol Cell Biol 27(8):2800-11
2)	Bengtson MH and Joazeiro CA (2010) Role of a ribosome-associated E3 ubiquitin ligase in protein quality control. Nature 467(7314):470-3
3)	Fleischer TC, et al. (2006) Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes. Genes Dev 20(10):1294-307
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41