ZRC1/YMR243C Protein Information Help

Standard Name Zrc1p
Systematic Name Ymr243cp
Alias Osr1p
ORF Classification Verified
Description Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description Zinc Resistance Conferring 6
Experimental Data
Molecules/cell 8200 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 442
Molecular Weight (Da) 48,344
Isoelectric Point (pI) 6.34

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Zrc1p (InterPro)
Transmembrane Domains There are 6 total predicted transmembrane domains (TMHMM)
Signal Peptide(s) There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions There are 13 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013C47E | P20107
MIPS: YMR243C
NCBI: 1353181 | 51013877 | 5924031 | 6323899 | 736309 | NP_013970.1
GenBank/EMBL/DDBJ: DAA10144.1 | AY693213 | X17537 | Z48756
External Classifications TC: 2.A.4.2
Amino Acid Sequence (or in FASTA format)
 
       1  MITGKELRII SLLTLDTVFF LLEITIGYMS HSLALIADSF HMLNDIISLL
      51  VALWAVDVAK NRGPDAKYTY GWKRAEILGA LINAVFLIAL CFSIMIEALQ
     101  RLIEPQEIQN PRLVLYVGVA GLISNVVGLF LFHDHGSDSL HSHSHGSVES
     151  GNNDLDIESN ATHSHSHASL PNDNLAIDED AISSPGPSGQ IGEVLPQSVV
     201  NRLSNESQPL LNHDDHDHSH ESKKPGHRSL NMHGVFLHVL GDALGNIGVI
     251  AAALFIWKTE YSWRYYSDPI VSLIITIIIF SSALPLSRRA SRILLQATPS
     301  TISADQIQRE ILAVPGVIAV HDFHVWNLTE SIYIASIHVQ IDCAPDKFMS
     351  SAKLIRKIFH QHGIHSATVQ PEFVSGDVNE DIRRRFSIIA GGSPSSSQEA
     401  FDSHGNTEHG RKKRSPTAYG ATTASSNCIV DDAVNCNTSN CL*

external links for Zrc1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome TCDB

DIP YeastRC Structure Prediction (Seattle)

GeneMANIA

References cited on this page View Complete Literature Guide for Zrc1p
1) MacDiarmid CW, et al.  (2000) Zinc transporters that regulate vacuolar zinc storage in Saccharomyces cerevisiae. EMBO J 19(12):2845-55
2) Li L and Kaplan J  (1998) Defects in the yeast high affinity iron transport system result in increased metal sensitivity because of the increased expression of transporters with a broad transition metal specificity. J Biol Chem 273(35):22181-7
3) MacDiarmid CW, et al.  (2003) Induction of the ZRC1 metal tolerance gene in zinc-limited yeast confers resistance to zinc shock. J Biol Chem 278(17):15065-72
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Tuschl K, et al.  (2012) Syndrome of hepatic cirrhosis, dystonia, polycythemia, and hypermanganesemia caused by mutations in SLC30A10, a manganese transporter in man. Am J Hum Genet 90(3):457-66
6) Kamizono A, et al.  (1989) Identification of a gene conferring resistance to zinc and cadmium ions in the yeast Saccharomyces cerevisiae. Mol Gen Genet 219(1-2):161-7
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41