RNH1/YMR234W Protein Information

Standard Name	Rnh1p
Systematic Name	Ymr234wp
ORF Classification	Verified
Description	Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) (1, 2, 3)
Name Description	RNase H

Experimental Data
Molecules/cell	149 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	348
Molecular Weight (Da)	39,431
Isoelectric Point (pI)	10.11

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rnh1p (InterPro)
Physical Interactions	There are 4 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000531D3 \| Q04740 MIPS: YMR234W NCBI: 1350805 \| 159162900 \| 4357 \| 6323890 \| 887615 \| NP_013961.1 \| NM_001182741.1 GenBank/EMBL/DDBJ: DAA10134.1 \| X57160 \| Z49939
External Classifications	EC: 3.1.26.4 [Ribonuclease H]

Amino Acid Sequence (or in FASTA format)
1 MARQGNFYAV RKGRETGIYN TWNECKNQVD GYGGAIYKKF NSYEQAKSFL 51 GQPNTTSNYG SSTHAGGQVS KPHTTQKRVH RRNRPLHYSS LTSSSACSSL 101 SSANTNTFYS VKSNVPNIES KIFNNWKDCQ AYVKHKRGIT FKKFEDQLAA 151 ENFISGMSAH DYKLMNISKE SFESKYKLSS NTMYNKSMNV YCDGSSFGNG 201 TSSSRAGYGA YFEGAPEENI SEPLLSGAQT NNRAEIEAVS EALKKIWEKL 251 TNEKEKVNYQ IKTDSEYVTK LLNDRYMTYD NKKLEGLPNS DLIVPLVQRF 301 VKVKKYYELN KECFKNNGKF QIEWVKGHDG DPGNEMADFL AKKGASRR*

Amino Acid Sequence (or in FASTA format)

       1  MARQGNFYAV RKGRETGIYN TWNECKNQVD GYGGAIYKKF NSYEQAKSFL
      51  GQPNTTSNYG SSTHAGGQVS KPHTTQKRVH RRNRPLHYSS LTSSSACSSL
     101  SSANTNTFYS VKSNVPNIES KIFNNWKDCQ AYVKHKRGIT FKKFEDQLAA
     151  ENFISGMSAH DYKLMNISKE SFESKYKLSS NTMYNKSMNV YCDGSSFGNG
     201  TSSSRAGYGA YFEGAPEENI SEPLLSGAQT NNRAEIEAVS EALKKIWEKL
     251  TNEKEKVNYQ IKTDSEYVTK LLNDRYMTYD NKKLEGLPNS DLIVPLVQRF
     301  VKVKKYYELN KECFKNNGKF QIEWVKGHDG DPGNEMADFL AKKGASRR*

external links for Rnh1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Rnh1p

1)	Cerritelli SM and Crouch RJ (1995) The non-RNase H domain of Saccharomyces cerevisiae RNase H1 binds double-stranded RNA: magnesium modulates the switch between double-stranded RNA binding and RNase H activity. RNA 1(3):246-59
2)	Itaya M, et al. (1991) Selective cloning of genes encoding RNase H from Salmonella typhimurium, Saccharomyces cerevisiae and Escherichia coli rnh mutant. Mol Gen Genet 227(3):438-45
3)	Huet J, et al. (1977) Characterization of ribonuclease H activity associated yeast RNA polymerase A. J Biol Chem 252(24):8848-55
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41