PEP5/YMR231W Protein Information

Standard Name	Pep5p 1
Systematic Name	Ymr231wp
Alias	End1p 2 , Vam1p , Vpl9p 3 , Vps11p 3, 4 , Vpt11p 4
ORF Classification	Verified
Description	Histone E3 ligase, component of CORVET tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p; involved in ubiquitylation and degradation of excess histones (5, 6, 7, 8)
Name Description	carboxyPEPtidase Y-deficient 9

Experimental Data
Molecules/cell	1200 10

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,029
Molecular Weight (Da)	117,476
Isoelectric Point (pI)	5.67

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pep5p (InterPro)
Physical Interactions	There are 102 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052FFA \| P12868 MIPS: YMR231W NCBI: 129788 \| 3680 \| 6323887 \| 666102 \| 887612 \| NP_013958.1 GenBank/EMBL/DDBJ: DAA10131.1 \| X15355 \| X54466 \| Z49939

Amino Acid Sequence (or in FASTA format)
1 MSLSSWRQFQ LFENIPIRDP NFGGDSLLYS DPTLCAATIV DPQTLIIAVN 51 SNIIKVVKLN QSQVIHEFQS FPHDFQITFL KVINGEFLVA LAESIGKPSL 101 IRVYKLEKLP NREQLYHSQV ELKNGNNTYP ISVVSISNDL SCIVVGFING 151 KIILIRGDIS RDRGSQQRII YEDPSKEPIT ALFLNNDATA CFAATTSRIL 201 LFNTTGRNRG RPSLVLNSKN GLDLNCGSFN PATNEFICCL SNFIEFFSSS 251 GKKHQFAFDL SLRKRIFCVD KDHILIVTEE TGVPTTSISV NELSPTIINR 301 IFIIDAKNKI ISLNFVVSSA IIDIFSTSQS GKNITYLLTS EGVMHRITPK 351 SLENQINIII QKELYPFALQ LAKQHSLSPL DVQEIHKKYG DYLFKKGLRK 401 EATDQYIQCL DVVETSEIIS KFGVKEVPDP ESMRNLADYL WSLIKNSISQ 451 RDHVTLLLIV LIKLKDVEGI DTFIQHFDRK GIWNEGVVMD DMDDVTFFYS 501 DNDFFDLDLI LELMKESDFK RLSYRLAKKY SKDSLIIVDI LLNLLHNPVK 551 AIKYIKSLPI DETLRCLVTY SKKLLEESPN ETNALLIEVF TGKFKPSTFE 601 VDLDRRDTTG DFSENIRTVF YSYKTFFNYM NSNGTSDAMS ESSEASHEHE 651 EPTYHPPKPS IVFSSFVTKP FEFVVFLEAC LACYQQYEGF DEDRQVILTT 701 LYDLYLNLAQ NDVPERIDDW RSRATGVLRE SNKLVYSAAS NNTSKRVDNS 751 IMLLISHMDQ SSASAKDKTK IDIASFANDN PEMDLLSTFR AMTLNEEPST 801 CLKFLEKYGT EEPKLLQVAL SYFVSNKLIF KEMGGNEVLK EKVLRPIIEG 851 ERMPLLDIIK ALSRTNVAHF GLIQDIIIDH VKTEDTEIKR NEKLIESYDK 901 ELKEKNKKLK NTINSDQPLH VPLKNQTCFM CRLTLDIPVV FFKCGHIYHQ 951 HCLNEEEDTL ESERKLFKCP KCLVDLETSN KLFEAQHEVV EKNDLLNFAL 1001 NSEEGSRDRF KVITEFLGRG AISYSDITI*

Amino Acid Sequence (or in FASTA format)

       1  MSLSSWRQFQ LFENIPIRDP NFGGDSLLYS DPTLCAATIV DPQTLIIAVN
      51  SNIIKVVKLN QSQVIHEFQS FPHDFQITFL KVINGEFLVA LAESIGKPSL
     101  IRVYKLEKLP NREQLYHSQV ELKNGNNTYP ISVVSISNDL SCIVVGFING
     151  KIILIRGDIS RDRGSQQRII YEDPSKEPIT ALFLNNDATA CFAATTSRIL
     201  LFNTTGRNRG RPSLVLNSKN GLDLNCGSFN PATNEFICCL SNFIEFFSSS
     251  GKKHQFAFDL SLRKRIFCVD KDHILIVTEE TGVPTTSISV NELSPTIINR
     301  IFIIDAKNKI ISLNFVVSSA IIDIFSTSQS GKNITYLLTS EGVMHRITPK
     351  SLENQINIII QKELYPFALQ LAKQHSLSPL DVQEIHKKYG DYLFKKGLRK
     401  EATDQYIQCL DVVETSEIIS KFGVKEVPDP ESMRNLADYL WSLIKNSISQ
     451  RDHVTLLLIV LIKLKDVEGI DTFIQHFDRK GIWNEGVVMD DMDDVTFFYS
     501  DNDFFDLDLI LELMKESDFK RLSYRLAKKY SKDSLIIVDI LLNLLHNPVK
     551  AIKYIKSLPI DETLRCLVTY SKKLLEESPN ETNALLIEVF TGKFKPSTFE
     601  VDLDRRDTTG DFSENIRTVF YSYKTFFNYM NSNGTSDAMS ESSEASHEHE
     651  EPTYHPPKPS IVFSSFVTKP FEFVVFLEAC LACYQQYEGF DEDRQVILTT
     701  LYDLYLNLAQ NDVPERIDDW RSRATGVLRE SNKLVYSAAS NNTSKRVDNS
     751  IMLLISHMDQ SSASAKDKTK IDIASFANDN PEMDLLSTFR AMTLNEEPST
     801  CLKFLEKYGT EEPKLLQVAL SYFVSNKLIF KEMGGNEVLK EKVLRPIIEG
     851  ERMPLLDIIK ALSRTNVAHF GLIQDIIIDH VKTEDTEIKR NEKLIESYDK
     901  ELKEKNKKLK NTINSDQPLH VPLKNQTCFM CRLTLDIPVV FFKCGHIYHQ
     951  HCLNEEEDTL ESERKLFKCP KCLVDLETSN KLFEAQHEVV EKNDLLNFAL
    1001  NSEEGSRDRF KVITEFLGRG AISYSDITI*

external links for Pep5p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Pep5p

1)	Woolford, C., et al. (1989) Personal Communication, Mortimer Map Edition 10
2)	Chvatchko Y, et al. (1986) Two yeast mutants defective in endocytosis are defective in pheromone response. Cell 46(3):355-64
3)	Rothman JH, et al. (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
4)	Robinson JS, et al. (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
5)	Srivastava A, et al. (2000) Pep3p/Pep5p complex: a putative docking factor at multiple steps of vesicular transport to the vacuole of Saccharomyces cerevisiae. Genetics 156(1):105-22
6)	Dulic V and Riezman H (1989) Characterization of the END1 gene required for vacuole biogenesis and gluconeogenic growth of budding yeast. EMBO J 8(5):1349-59
7)	Peplowska K, et al. (2007) The CORVET Tethering Complex Interacts with the Yeast Rab5 Homolog Vps21 and Is Involved in Endo-Lysosomal Biogenesis. Dev Cell 12(5):739-50
8)	Singh RK, et al. (2012) Novel E3 Ubiquitin Ligases That Regulate Histone Protein Levels in the Budding Yeast Saccharomyces cerevisiae. PLoS One 7(5):e36295
9)	Jones EW (1977) Proteinase mutants of Saccharomyces cerevisiae. Genetics 85(1):23-33
10)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41