MTF1/YMR228W Protein Information

Standard Name	Mtf1p
Systematic Name	Ymr228wp
ORF Classification	Verified
Description	Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation (1, 2, 3, 4, 5)
Name Description	Mitochondrial Transcription Factor 6

Experimental Data
Molecules/cell	9380 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	341
Molecular Weight (Da)	39,726
Isoelectric Point (pI)	6.52

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Mtf1p (InterPro)
Physical Interactions	There are 16 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001683E2 \| P14908 MIPS: YMR228W NCBI: 15988002 \| 1709156 \| 4310 \| 6323884 \| 887609 \| NP_013955.1 GenBank/EMBL/DDBJ: DAA10127.1 \| X13513 \| Z49939

Amino Acid Sequence (or in FASTA format)
1 MSVPIPGIKD ISKLKFFYGF KYLWNPTVYN KIFDKLDLTK TYKHPEELKV 51 LDLYPGVGIQ SAIFYNKYCP RQYSLLEKRS SLYKFLNAKF EGSPLQILKR 101 DPYDWSTYSN LIDEERIFVP EVQSSDHIND KFLTVANVTG EGSEGLIMQW 151 LSCIGNKNWL YRFGKVKMLL WMPSTTARKL LARPGMHSRS KCSVVREAFT 201 DTKLIAISDA NELKGFDSQC IEEWDPILFS AAEIWPTKGK PIALVEMDPI 251 DFDFDVDNWD YVTRHLMILK RTPLNTVMDS LGHGGQQYFN SRITDKDLLK 301 KCPIDLTNDE FIYLTKLFME WPFKPDILMD FVDMYQTEHS G*

Amino Acid Sequence (or in FASTA format)

       1  MSVPIPGIKD ISKLKFFYGF KYLWNPTVYN KIFDKLDLTK TYKHPEELKV
      51  LDLYPGVGIQ SAIFYNKYCP RQYSLLEKRS SLYKFLNAKF EGSPLQILKR
     101  DPYDWSTYSN LIDEERIFVP EVQSSDHIND KFLTVANVTG EGSEGLIMQW
     151  LSCIGNKNWL YRFGKVKMLL WMPSTTARKL LARPGMHSRS KCSVVREAFT
     201  DTKLIAISDA NELKGFDSQC IEEWDPILFS AAEIWPTKGK PIALVEMDPI
     251  DFDFDVDNWD YVTRHLMILK RTPLNTVMDS LGHGGQQYFN SRITDKDLLK
     301  KCPIDLTNDE FIYLTKLFME WPFKPDILMD FVDMYQTEHS G*

external links for Mtf1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Mtf1p

1)	Mangus DA, et al. (1994) Release of the yeast mitochondrial RNA polymerase specificity factor from transcription complexes. J Biol Chem 269(42):26568-74
2)	Schubot FD, et al. (2001) Crystal structure of the transcription factor sc-mtTFB offers insights into mitochondrial transcription. Protein Sci 10(10):1980-8
3)	Matsunaga M and Jaehning JA (2004) Intrinsic promoter recognition by a "core" RNA polymerase. J Biol Chem 279(43):44239-42
4)	Tang GQ, et al. (2011) Transcription factor-dependent DNA bending governs promoter recognition by the mitochondrial RNA polymerase. J Biol Chem 286(44):38805-13
5)	Velazquez G, et al. (2012) Conservation of promoter melting mechanisms in divergent regions of the single-subunit RNA polymerases. Biochemistry 51(18):3901-10
6)	Lisowsky T and Michaelis G (1989) Mutations in the genes for mitochondrial RNA polymerase and a second mitochondrial transcription factor of Saccharomyces cerevisiae. Mol Gen Genet 219(1-2):125-8
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41