SKY1/YMR216C Protein Information

Standard Name	Sky1p 1
Systematic Name	Ymr216cp
ORF Classification	Verified
Description	SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 (1, 2)
Name Description	SRPK1-like Kinase in Yeast 1

Experimental Data
Molecules/cell	2420 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	742
Molecular Weight (Da)	83,237
Isoelectric Point (pI)	6.18

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Sky1p (InterPro)
Physical Interactions	There are 76 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012DEF9 \| Q03656 MIPS: YMR216C NCBI: 126031131 \| 126031132 \| 13399615 \| 2499619 \| 37928043 \| 37928044 \| 37928047 \| 37928048 \| 37928051 \| 37928052 \| 37928056 \| 37928057 \| 6323872 \| 854468 \| NP_013943.1 GenBank/EMBL/DDBJ: DAA10115.1 \| Z49809
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MGSSINYPGF VTKSAHLADT STDASISCEE ATSSQEAKKN FFQRDYNMMK 51 KAPAPTKSKL SLALQTSKSS SSANGTVQED TSSKTEDFST KSIKKKPDSG 101 VESHVSIQSD SGPQSDSDLD SDSSISSCDE RNEESLKDYR PGGYHPAFKG 151 EPYKDARYIL VRKLGWGHFS TVWLAKDMVN NTHVAMKIVR GDKVYTEAAE 201 DEIKLLQRVN DADNTKEDSM GANHILKLLD HFNHKGPNGV HVVMVFEVLG 251 ENLLALIKKY EHRGIPLIYV KQISKQLLLG LDYMHRRCGI IHTDIKPENV 301 LMEIGDVEGI VQMVEALDKQ KREAKRLQRH VSRSSDITAN DSSDEKWAEC 351 QTSMPCGSSS NSKSRSIEKD LSKRCFRRPR RHTIITGSQP LPSPISSSNF 401 FEMRAHFCGS SHNSFSSVSG NRNIPSSINN NSINNGIGIK NSNNSFLNSV 451 PHSVTRMFIN EDSNDNNNND NSKNKNNNNN NSNNNNNEDI MNTPLHEEQL 501 ADSLSTFDIS NISQSSDTNG PYISNTMDSN SNVSTDINSP ENLIQIKIAD 551 LGNACWYDEH YTNSIQTREY RSPEVLLGAP WGCGADIWST ACLIFELITG 601 DFLFEPDEGH SYTKDDDHIA QIIELLGELP SYLLRNGKYT RTFFNSRGLL 651 RNISKLKFWP LEDVLTEKYK FSKDEAKEIS DFLSPMLQLD PRKRADAGGL 701 VNHPWLKDTL GMEEIRVPDR ELYGSGSDIP GWFEEVRDHK RH*

Amino Acid Sequence (or in FASTA format)

       1  MGSSINYPGF VTKSAHLADT STDASISCEE ATSSQEAKKN FFQRDYNMMK
      51  KAPAPTKSKL SLALQTSKSS SSANGTVQED TSSKTEDFST KSIKKKPDSG
     101  VESHVSIQSD SGPQSDSDLD SDSSISSCDE RNEESLKDYR PGGYHPAFKG
     151  EPYKDARYIL VRKLGWGHFS TVWLAKDMVN NTHVAMKIVR GDKVYTEAAE
     201  DEIKLLQRVN DADNTKEDSM GANHILKLLD HFNHKGPNGV HVVMVFEVLG
     251  ENLLALIKKY EHRGIPLIYV KQISKQLLLG LDYMHRRCGI IHTDIKPENV
     301  LMEIGDVEGI VQMVEALDKQ KREAKRLQRH VSRSSDITAN DSSDEKWAEC
     351  QTSMPCGSSS NSKSRSIEKD LSKRCFRRPR RHTIITGSQP LPSPISSSNF
     401  FEMRAHFCGS SHNSFSSVSG NRNIPSSINN NSINNGIGIK NSNNSFLNSV
     451  PHSVTRMFIN EDSNDNNNND NSKNKNNNNN NSNNNNNEDI MNTPLHEEQL
     501  ADSLSTFDIS NISQSSDTNG PYISNTMDSN SNVSTDINSP ENLIQIKIAD
     551  LGNACWYDEH YTNSIQTREY RSPEVLLGAP WGCGADIWST ACLIFELITG
     601  DFLFEPDEGH SYTKDDDHIA QIIELLGELP SYLLRNGKYT RTFFNSRGLL
     651  RNISKLKFWP LEDVLTEKYK FSKDEAKEIS DFLSPMLQLD PRKRADAGGL
     701  VNHPWLKDTL GMEEIRVPDR ELYGSGSDIP GWFEEVRDHK RH*

external links for Sky1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
YGOB	CYC2008 (complexes)	Pfam domains
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Sky1p

1)	Siebel CW, et al. (1999) Conservation in budding yeast of a kinase specific for SR splicing factors. Proc Natl Acad Sci U S A 96(10):5440-5
2)	Forment J, et al. (2002) The yeast SR protein kinase Sky1p modulates salt tolerance, membrane potential and the Trk1,2 potassium transporter. Biochim Biophys Acta 1565(1):36-40
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41