GAS3/YMR215W Protein Information

Standard Name	Gas3p 1
Systematic Name	Ymr215wp
ORF Classification	Verified
Description	Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation (1, 2, 3, 4)
Name Description	Glycophospholipid-Anchored Surface protein 5

Experimental Data
Molecules/cell	16400 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	524
Molecular Weight (Da)	56,793
Isoelectric Point (pI)	4.43

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Gas3p (InterPro)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 49 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012B0E4 \| Q03655 MIPS: YMR215W NCBI: 2494678 \| 51013855 \| 6323871 \| 854467 \| NP_013942.1 GenBank/EMBL/DDBJ: DAA10114.1 \| AY693202 \| Z49809

Amino Acid Sequence (or in FASTA format)
1 MQLSKSILLA ALAATPSLVN AMLPIHIKNY RFIKPSSATN SESDNEVFFV 51 KGVDYQPGGS SGYDADSDTD ILSDPEVCAR DAYAFQQLGV NTVRIYSLNP 101 DLNHDKCMTI FNNAGIYAIL DVNSGNYGES LNRADPSGTY DSLYLSRVFK 151 FIDAFKNYPN VLGFFSGNEV INDQSDYAKI DPPYIRAVQR DMKQYISKHA 201 NRSIPVGYSA ADNTDLRLAT FKYLQCNSLD GNKVNDDLDI SKSDFFGLNT 251 YEWCSGTSSW ESSGYDKLNS TFEDAVIPLI FSEYGCNKNT PRTFDEVSEG 301 LYGGLKNVFS GGLVYEYTEE ANNYGLVKLD DSGSLTYKDD FVNLESQLKN 351 VSLPTTKESE ISSDSIYKCD NSAITNIYSG FGTNNFTLPS QPAEIANMIE 401 YGVNGTNTGK ILTDYAVPTT FNYTIKNNKD DTISATISYD KANSLNELDV 451 TATTVAKSAS TSQSSSRSLT SSTSPSSSTG SSSSTGSSSA SSSSKSKGVG 501 NIVNVSFSQS GYLALFAGLI SALL*

Amino Acid Sequence (or in FASTA format)

       1  MQLSKSILLA ALAATPSLVN AMLPIHIKNY RFIKPSSATN SESDNEVFFV
      51  KGVDYQPGGS SGYDADSDTD ILSDPEVCAR DAYAFQQLGV NTVRIYSLNP
     101  DLNHDKCMTI FNNAGIYAIL DVNSGNYGES LNRADPSGTY DSLYLSRVFK
     151  FIDAFKNYPN VLGFFSGNEV INDQSDYAKI DPPYIRAVQR DMKQYISKHA
     201  NRSIPVGYSA ADNTDLRLAT FKYLQCNSLD GNKVNDDLDI SKSDFFGLNT
     251  YEWCSGTSSW ESSGYDKLNS TFEDAVIPLI FSEYGCNKNT PRTFDEVSEG
     301  LYGGLKNVFS GGLVYEYTEE ANNYGLVKLD DSGSLTYKDD FVNLESQLKN
     351  VSLPTTKESE ISSDSIYKCD NSAITNIYSG FGTNNFTLPS QPAEIANMIE
     401  YGVNGTNTGK ILTDYAVPTT FNYTIKNNKD DTISATISYD KANSLNELDV
     451  TATTVAKSAS TSQSSSRSLT SSTSPSSSTG SSSSTGSSSA SSSSKSKGVG
     501  NIVNVSFSQS GYLALFAGLI SALL*

external links for Gas3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
YGOB	CYC2008 (complexes)	Pfam domains
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Gas3p

1)	Popolo L and Vai M (1999) The Gas1 glycoprotein, a putative wall polymer cross-linker. Biochim Biophys Acta 1426(2):385-400
2)	Hamada K, et al. (1999) Amino acid residues in the omega-minus region participate in cellular localization of yeast glycosylphosphatidylinositol-attached proteins. J Bacteriol 181(13):3886-9
3)	Mouyna I, et al. (2000) Identification of the catalytic residues of the first family of beta(1-3)glucanosyltransferases identified in fungi. Biochem J 347 Pt 3():741-7
4)	Rolli E, et al. (2010) GAS3, a developmentally regulated gene, encodes a highly mannosylated and inactive protein of the Gas family of Saccharomyces cerevisiae. Yeast 27(8):597-610
5)	Nuoffer C, et al. (1991) Determinants for glycophospholipid anchoring of the Saccharomyces cerevisiae GAS1 protein to the plasma membrane. Mol Cell Biol 11(1):27-37
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41