CEF1/YMR213W Protein Information

Standard Name	Cef1p 1
Systematic Name	Ymr213wp
Alias	Ntc85p 2
ORF Classification	Verified
Description	Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p (1, 2)
Name Description	CErevisiae homolog of cdc Five 1

Experimental Data
Molecules/cell	784 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	590
Molecular Weight (Da)	67,730
Isoelectric Point (pI)	8.62

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Cef1p (InterPro)
Physical Interactions	There are 158 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000531EB \| Q03654 MIPS: YMR213W NCBI: 2498003 \| 51013735 \| 6323869 \| 854465 \| NP_013940.1 \| NM_001182720.1 GenBank/EMBL/DDBJ: DAA10112.1 \| AY693142 \| Z49809

Amino Acid Sequence (or in FASTA format)
1 MPPVPIYVKG GVWTNVEDQI LKAAVQKYGT HQWSKVASLL QKKTARQSEL 51 RWNEYLNPKL NFTEFSKEED AQLLDLAREL PNQWRTIADM MARPAQVCVE 101 RYNRLLESED SGGAALSTGV TDLKAGDINP NAETQMARPD NGDLEDEEKE 151 MLAEARARLL NTQGKKATRK IRERMLEESK RIAELQKRRE LKQAGINVAI 201 KKPKKKYGTD IDYNEDIVYE QAPMPGIYDT STEDRQIKKK FEQFERKVNR 251 KGLDGNKDKP SKKNKDKKRK HDENEHVEKA ALGESTTLTD EYKKPKLILS 301 APGTKQGKVT YKKKLESKRQ KLIEAQATGT VLTPKELLPH DSGQEDNERS 351 NIKSGKQLKS RIRKFLVQMF ASLPSPKNDF EIVLSEDEKE EDAEIAEYEK 401 EFENERAMNE EDNFIEPPSQ NDAPRVSLVA VPLAYSTLPI PEFKNNPQSA 451 IDNKYNLLVA NAINKEPHMV PEDTVDFLKE VESRMQHITQ GRTSMKIQFK 501 TAMPPTEVLL ESIQSKVESI EQLQRKLQHV QPLEQQNNEM CSTLCHHSLP 551 ALIEGQRKYY ADYYAYRQEI RSLEGRRKRL QAMLNSSSSI *

Amino Acid Sequence (or in FASTA format)

       1  MPPVPIYVKG GVWTNVEDQI LKAAVQKYGT HQWSKVASLL QKKTARQSEL
      51  RWNEYLNPKL NFTEFSKEED AQLLDLAREL PNQWRTIADM MARPAQVCVE
     101  RYNRLLESED SGGAALSTGV TDLKAGDINP NAETQMARPD NGDLEDEEKE
     151  MLAEARARLL NTQGKKATRK IRERMLEESK RIAELQKRRE LKQAGINVAI
     201  KKPKKKYGTD IDYNEDIVYE QAPMPGIYDT STEDRQIKKK FEQFERKVNR
     251  KGLDGNKDKP SKKNKDKKRK HDENEHVEKA ALGESTTLTD EYKKPKLILS
     301  APGTKQGKVT YKKKLESKRQ KLIEAQATGT VLTPKELLPH DSGQEDNERS
     351  NIKSGKQLKS RIRKFLVQMF ASLPSPKNDF EIVLSEDEKE EDAEIAEYEK
     401  EFENERAMNE EDNFIEPPSQ NDAPRVSLVA VPLAYSTLPI PEFKNNPQSA
     451  IDNKYNLLVA NAINKEPHMV PEDTVDFLKE VESRMQHITQ GRTSMKIQFK
     501  TAMPPTEVLL ESIQSKVESI EQLQRKLQHV QPLEQQNNEM CSTLCHHSLP
     551  ALIEGQRKYY ADYYAYRQEI RSLEGRRKRL QAMLNSSSSI *

external links for Cef1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Cef1p

1)	Ohi R, et al. (1998) Myb-related Schizosaccharomyces pombe cdc5p is structurally and functionally conserved in eukaryotes. Mol Cell Biol 18(7):4097-108
2)	Tsai WY, et al. (1999) Cef1p is a component of the Prp19p-associated complex and essential for pre-mRNA splicing. J Biol Chem 274(14):9455-62
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41