HFA1/YMR207C Protein Information

Standard Name	Hfa1p
Systematic Name	Ymr207cp
ORF Classification	Verified
Description	Mitochondrial acetyl-coenzyme A carboxylase; catalyzes production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress; HFA1 has a paralog, ACC1, that arose from the whole genome duplication (1, 2, 3, 4, 5)

Experimental Data
Molecules/cell	396 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	2,123
Molecular Weight (Da)	241,784
Isoelectric Point (pI)	8.05

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Hfa1p (InterPro)
Physical Interactions	There are 13 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012C66F \| P32874 MIPS: YMR207C NCBI: 1326008 \| 1708192 \| 2804173 \| 388250 \| 6323863 \| 763183 \| NP_013934.1 \| NM_001182714.1 GenBank/EMBL/DDBJ: DAA10106.1 \| D78165 \| Z22558 \| Z48755 \| Z49809
External Classifications	EC: 6.3.4.14 [Biotin carboxylase] EC: 6.4.1.2 [Acetyl-CoA carboxylase]

Amino Acid Sequence (or in FASTA format)
1 MRSIRKWAYE TFNDEKIIQF VVMATPDDLH ANSEYIRMAD QYVQVPGGTN 51 NNNYANIDLI LDVAEQTDVD AVWAGWGHAS ENPCLPELLA SSQRKILFIG 101 PPGRAMRSLG DKISSTIVAQ SAKIPCIPWS GSHIDTIHID NKTNFVSVPD 151 DVYVRGCCSS PEDALEKAKL IGFPVMIKAS EGGGGKGIRR VDNEDDFIAL 201 YRQAVNETPG SPMFVMKVVT DARHLEVQLL ADQYGTNITL FGRDCSIQRR 251 HQKIIEEAPV TITKPETFQR MERAAIRLGE LVGYVSAGTV EYLYSPKDDK 301 FYFLELNPRL QVEHPTTEMI SGVNLPATQL QIAMGIPMHM ISDIRKLYGL 351 DPTGTSYIDF KNLKRPSPKG HCISCRITSE DPNEGFKPST GKIHELNFRS 401 SSNVWGYFSV GNNGAIHSFS DSQFGHIFAV GNDRQDAKQN MVLALKDFSI 451 RGEFKTPIEY LIELLETRDF ESNNISTGWL DDLILKNLSS DSKLDPTLAI 501 ICGAAMKAYV FTEKVRNKYL ELLRRGQVPP KDFLKTKFPV DFIFDNNRYL 551 FNVAQSSEEQ FILSINKSQC EVNVQKLSSD CLLISVDGKC HTVYWKDDIR 601 GTRLSIDSNT IFLEAELNPT QVISPTPGKL VKYLVRSGDH VFAGQQYAEI 651 EIMKMQMPLV AKSDGVIELL RQPGSIIEAG DVIAKLTLDS PSKANESSLY 701 RGELPVLGPP LIEGSRPNHK LRVLINRLEN ILNGYHENSG IETTLKELIK 751 ILRDGRLPYS EWDSQISTVR NRLPRQLNEG LGNLVKKSVS FPAKELHKLM 801 KRYLEENTND HVVYVALQPL LKISERYSEG LANHECEIFL KLIKKYYAVE 851 KIFENHDIHE ERNLLNLRRK DLTNLKKILC ISLSHANVVA KNKLVTAILH 901 EYEPLCQDSS KMSLKFRAVI HDLASLESKW AKEVAVKARS VLLRGIFPPI 951 KKRKEHIKTL LQLHIKDTGA ENIHSRNIYS CMRDFGNLIH SNLIQLQDLF 1001 FFFGHQDTAL SSIASEIYAR YAYGNYQLKS IKIHKGAPDL LMSWQFSSLR 1051 NYLVNSDGES DEFTKLSKPP STSGKSSANS FGLLVNMRAL ESLEKTLDEV 1101 YEQIHIPEER LSSGENSLIV NILSPIRYRS ENDLIKTLKI KLHENERGLS 1151 KLKVNRITFA FIAANAPAVK FYSFDGTTYD EISQIRNMDP SYEAPLELGK 1201 MSNYKIRSLP TYDSSIRIFE GISKFTPLDK RFFVRKIINS FMYNDQKTTE 1251 ENLKAEINAQ VVYMLEHLGA VDISNSDLNH IFLSFNTVLN IPVHRLEEIV 1301 STILKTHETR LFQERITDVE ICISVECLET KKPAPLRLLI SNKSGYVVKI 1351 ETYYEKIGKN GNLILEPCSE QSHYSQKSLS LPYSVKDWLQ PKRYKAQFMG 1401 TTYVYDFPGL FHQAAIQQWK RYFPKHKLND SFFSWVELIE QNGNLIKVNR 1451 EPGLNNIGMV AFEIMVQTPE YPEGRNMIVI SNDITYNIGS FGPREDLFFD 1501 RVTNYARERG IPRIYLAANS GAKLGIAEEL IPLFRVAWND PSDPTKGFQY 1551 LYLAPKDMQL LKDSGKGNSV VVEHKMVYGE ERYIIKAIVG FEEGLGVECL 1601 QGSGLIAGAT SKAYRDIFTI TAVTCRSVGI GSYLVRLGQR TIQVEDKPII 1651 LTGASAINKV LGTDIYTSNL QIGGTQIMYK NGIAHLTASN DMKAIEKIMT 1701 WLSYVPAKRD MSPPLLETMD RWDRDVDFKP AKQVPYEARW LIEGKWDSNN 1751 NFQSGLFDKD SFFETLSGWA KGVIVGRARL GGIPVGVIAV ETKTIEEIIP 1801 ADPANLDSSE FSVKEAGQVW YPNSAFKTAQ TINDFNYGEQ LPLIILANWR 1851 GFSGGQRDMY NEVLKYGSFI VDALVDYKQP ILIYIPPFGE LRGGSWVVID 1901 PTINPEQMEM YADVESRGGV LEPDGVVSIK YRKEKMIETM IRLDSTYGHL 1951 RRTLTEKKLS LEKQNDLTKR LKIRERQLIP IYNQISIQFA DLHDRSTRML 2001 VKGVIRNELE WKKSRRFLYW RLRRRLNEGQ VIKRLQKKTC DNKTKMKYDD 2051 LLKIVQSWYN DLDVNDDRAV VEFIERNSKK IDKNIEEFEI SLLIDELKKK 2101 FEDRRGNIVL EELTRLVDSK RKR*

Amino Acid Sequence (or in FASTA format)

       1  MRSIRKWAYE TFNDEKIIQF VVMATPDDLH ANSEYIRMAD QYVQVPGGTN
      51  NNNYANIDLI LDVAEQTDVD AVWAGWGHAS ENPCLPELLA SSQRKILFIG
     101  PPGRAMRSLG DKISSTIVAQ SAKIPCIPWS GSHIDTIHID NKTNFVSVPD
     151  DVYVRGCCSS PEDALEKAKL IGFPVMIKAS EGGGGKGIRR VDNEDDFIAL
     201  YRQAVNETPG SPMFVMKVVT DARHLEVQLL ADQYGTNITL FGRDCSIQRR
     251  HQKIIEEAPV TITKPETFQR MERAAIRLGE LVGYVSAGTV EYLYSPKDDK
     301  FYFLELNPRL QVEHPTTEMI SGVNLPATQL QIAMGIPMHM ISDIRKLYGL
     351  DPTGTSYIDF KNLKRPSPKG HCISCRITSE DPNEGFKPST GKIHELNFRS
     401  SSNVWGYFSV GNNGAIHSFS DSQFGHIFAV GNDRQDAKQN MVLALKDFSI
     451  RGEFKTPIEY LIELLETRDF ESNNISTGWL DDLILKNLSS DSKLDPTLAI
     501  ICGAAMKAYV FTEKVRNKYL ELLRRGQVPP KDFLKTKFPV DFIFDNNRYL
     551  FNVAQSSEEQ FILSINKSQC EVNVQKLSSD CLLISVDGKC HTVYWKDDIR
     601  GTRLSIDSNT IFLEAELNPT QVISPTPGKL VKYLVRSGDH VFAGQQYAEI
     651  EIMKMQMPLV AKSDGVIELL RQPGSIIEAG DVIAKLTLDS PSKANESSLY
     701  RGELPVLGPP LIEGSRPNHK LRVLINRLEN ILNGYHENSG IETTLKELIK
     751  ILRDGRLPYS EWDSQISTVR NRLPRQLNEG LGNLVKKSVS FPAKELHKLM
     801  KRYLEENTND HVVYVALQPL LKISERYSEG LANHECEIFL KLIKKYYAVE
     851  KIFENHDIHE ERNLLNLRRK DLTNLKKILC ISLSHANVVA KNKLVTAILH
     901  EYEPLCQDSS KMSLKFRAVI HDLASLESKW AKEVAVKARS VLLRGIFPPI
     951  KKRKEHIKTL LQLHIKDTGA ENIHSRNIYS CMRDFGNLIH SNLIQLQDLF
    1001  FFFGHQDTAL SSIASEIYAR YAYGNYQLKS IKIHKGAPDL LMSWQFSSLR
    1051  NYLVNSDGES DEFTKLSKPP STSGKSSANS FGLLVNMRAL ESLEKTLDEV
    1101  YEQIHIPEER LSSGENSLIV NILSPIRYRS ENDLIKTLKI KLHENERGLS
    1151  KLKVNRITFA FIAANAPAVK FYSFDGTTYD EISQIRNMDP SYEAPLELGK
    1201  MSNYKIRSLP TYDSSIRIFE GISKFTPLDK RFFVRKIINS FMYNDQKTTE
    1251  ENLKAEINAQ VVYMLEHLGA VDISNSDLNH IFLSFNTVLN IPVHRLEEIV
    1301  STILKTHETR LFQERITDVE ICISVECLET KKPAPLRLLI SNKSGYVVKI
    1351  ETYYEKIGKN GNLILEPCSE QSHYSQKSLS LPYSVKDWLQ PKRYKAQFMG
    1401  TTYVYDFPGL FHQAAIQQWK RYFPKHKLND SFFSWVELIE QNGNLIKVNR
    1451  EPGLNNIGMV AFEIMVQTPE YPEGRNMIVI SNDITYNIGS FGPREDLFFD
    1501  RVTNYARERG IPRIYLAANS GAKLGIAEEL IPLFRVAWND PSDPTKGFQY
    1551  LYLAPKDMQL LKDSGKGNSV VVEHKMVYGE ERYIIKAIVG FEEGLGVECL
    1601  QGSGLIAGAT SKAYRDIFTI TAVTCRSVGI GSYLVRLGQR TIQVEDKPII
    1651  LTGASAINKV LGTDIYTSNL QIGGTQIMYK NGIAHLTASN DMKAIEKIMT
    1701  WLSYVPAKRD MSPPLLETMD RWDRDVDFKP AKQVPYEARW LIEGKWDSNN
    1751  NFQSGLFDKD SFFETLSGWA KGVIVGRARL GGIPVGVIAV ETKTIEEIIP
    1801  ADPANLDSSE FSVKEAGQVW YPNSAFKTAQ TINDFNYGEQ LPLIILANWR
    1851  GFSGGQRDMY NEVLKYGSFI VDALVDYKQP ILIYIPPFGE LRGGSWVVID
    1901  PTINPEQMEM YADVESRGGV LEPDGVVSIK YRKEKMIETM IRLDSTYGHL
    1951  RRTLTEKKLS LEKQNDLTKR LKIRERQLIP IYNQISIQFA DLHDRSTRML
    2001  VKGVIRNELE WKKSRRFLYW RLRRRLNEGQ VIKRLQKKTC DNKTKMKYDD
    2051  LLKIVQSWYN DLDVNDDRAV VEFIERNSKK IDKNIEEFEI SLLIDELKKK
    2101  FEDRRGNIVL EELTRLVDSK RKR*

external links for Hfa1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Hfa1p

1)	Kearsey SE (1993) Identification of a Saccharomyces cerevisiae gene closely related to FAS3 (acetyl-CoA carboxylase) DNA Seq 4(1):69-70
2)	Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
3)	Hoja U, et al. (2004) HFA1 encoding an organelle-specific acetyl-CoA carboxylase controls mitochondrial fatty acid synthesis in Saccharomyces cerevisiae. J Biol Chem 279(21):21779-86
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41