SGS1/YMR190C Protein Information

Standard Name	Sgs1p 1
Systematic Name	Ymr190cp
ORF Classification	Verified
Description	Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress (2, 3, 4, 5, 6, 7, 8, 9)
Name Description	Slow Growth Suppressor

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,447
Molecular Weight (Da)	163,836
Isoelectric Point (pI)	6.27

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Sgs1p (InterPro)
Physical Interactions	There are 64 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000531CC \| P35187 MIPS: YMR190C NCBI: 349194 \| 464912 \| 6323844 \| 642282 \| 6980735 \| 726279 \| NP_013915.1 GenBank/EMBL/DDBJ: DAA10088.1 \| L07870 \| U22341 \| Z47815
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides] EC: 3.6.4.-

Amino Acid Sequence (or in FASTA format)
1 MVTKPSHNLR REHKWLKETA TLQEDKDFVF QAIQKHIANK RPKTNSPPTT 51 PSKDECGPGT TNFITSIPAS GPTNTATKQH EVMQTLSNDT EWLSYTATSN 101 QYADVPMVDI PASTSVVSNP RTPNGSKTHN FNTFRPHMAS SLVENDSSRN 151 LGSRNNNKSV IDNSSIGKQL ENDIKLEVIR LQGSLIMALK EQSKLLLQKC 201 SIIESTSLSE DAKRLQLSRD IRPQLSNMSI RIDSLEKEII KAKKDGMSKD 251 QSKGRSQVSS QDDNIISSIL PSPLEYNTSS RNSNLTSTTA TTVTKALAIT 301 GAKQNITNNT GKNSNNDSNN DDLIQVLDDE DDIDCDPPVI LKEGAPHSPA 351 FPHLHMTSEE QDELTRRRNM RSREPVNYRI PDRDDPFDYV MGKSLRDDYP 401 DVEREEDELT MEAEDDAHSS YMTTRDEEKE ENELLNQSDF DFVVNDDLDP 451 TQDTDYHDNM DVSANIQESS QEGDTRSTIT LSQNKNVQVI LSSPTAQSVP 501 SNGQNQIGVE HIDLLEDDLE KDAILDDSMS FSFGRQHMPM SHSDLELIDS 551 EKENEDFEED NNNNGIEYLS DSDLERFDEE RENRTQVADI QELDNDLKII 601 TERKLTGDNE HPPPSWSPKI KREKSSVSQK DEEDDFDDDF SLSDIVSKSN 651 LSSKTNGPTY PWSDEVLYRL HEVFKLPGFR PNQLEAVNAT LQGKDVFVLM 701 PTGGGKSLCY QLPAVVKSGK THGTTIVISP LISLMQDQVE HLLNKNIKAS 751 MFSSRGTAEQ RRQTFNLFIN GLLDLVYISP EMISASEQCK RAISRLYADG 801 KLARIVVDEA HCVSNWGHDF RPDYKELKFF KREYPDIPMI ALTATASEQV 851 RMDIIHNLEL KEPVFLKQSF NRTNLYYEVN KKTKNTIFEI CDAVKSRFKN 901 QTGIIYCHSK KSCEQTSAQM QRNGIKCAYY HAGMEPDERL SVQKAWQADE 951 IQVICATVAF GMGIDKPDVR FVYHFTVPRT LEGYYQETGR AGRDGNYSYC 1001 ITYFSFRDIR TMQTMIQKDK NLDRENKEKH LNKLQQVMAY CDNVTDCRRK 1051 LVLSYFNEDF DSKLCHKNCD NCRNSANVIN EERDVTEPAK KIVKLVESIQ 1101 NERVTIIYCQ DVFKGSRSSK IVQANHDTLE EHGIGKSMQK SEIERIFFHL 1151 ITIRVLQEYS IMNNSGFASS YVKVGPNAKK LLTGKMEIKM QFTISAPNSR 1201 PSTSSSFQAN EDNIPVIAQK STTIGGNVAA NPPRFISAKE HLRSYTYGGS 1251 TMGSSHPITL KNTSDLRSTQ ELNNLRMTYE RLRELSLNLG NRMVPPVGNF 1301 MPDSILKKMA AILPMNDSAF ATLGTVEDKY RRRFKYFKAT IADLSKKRSS 1351 EDHEKYDTIL NDEFVNRAAA SSNGIAQSTG TKSKFFGANL NEAKENEQII 1401 NQIRQSQLPK NTTSSKSGTR SISKSSKKSA NGRRGFRNYR GHYRGRK*

Amino Acid Sequence (or in FASTA format)

       1  MVTKPSHNLR REHKWLKETA TLQEDKDFVF QAIQKHIANK RPKTNSPPTT
      51  PSKDECGPGT TNFITSIPAS GPTNTATKQH EVMQTLSNDT EWLSYTATSN
     101  QYADVPMVDI PASTSVVSNP RTPNGSKTHN FNTFRPHMAS SLVENDSSRN
     151  LGSRNNNKSV IDNSSIGKQL ENDIKLEVIR LQGSLIMALK EQSKLLLQKC
     201  SIIESTSLSE DAKRLQLSRD IRPQLSNMSI RIDSLEKEII KAKKDGMSKD
     251  QSKGRSQVSS QDDNIISSIL PSPLEYNTSS RNSNLTSTTA TTVTKALAIT
     301  GAKQNITNNT GKNSNNDSNN DDLIQVLDDE DDIDCDPPVI LKEGAPHSPA
     351  FPHLHMTSEE QDELTRRRNM RSREPVNYRI PDRDDPFDYV MGKSLRDDYP
     401  DVEREEDELT MEAEDDAHSS YMTTRDEEKE ENELLNQSDF DFVVNDDLDP
     451  TQDTDYHDNM DVSANIQESS QEGDTRSTIT LSQNKNVQVI LSSPTAQSVP
     501  SNGQNQIGVE HIDLLEDDLE KDAILDDSMS FSFGRQHMPM SHSDLELIDS
     551  EKENEDFEED NNNNGIEYLS DSDLERFDEE RENRTQVADI QELDNDLKII
     601  TERKLTGDNE HPPPSWSPKI KREKSSVSQK DEEDDFDDDF SLSDIVSKSN
     651  LSSKTNGPTY PWSDEVLYRL HEVFKLPGFR PNQLEAVNAT LQGKDVFVLM
     701  PTGGGKSLCY QLPAVVKSGK THGTTIVISP LISLMQDQVE HLLNKNIKAS
     751  MFSSRGTAEQ RRQTFNLFIN GLLDLVYISP EMISASEQCK RAISRLYADG
     801  KLARIVVDEA HCVSNWGHDF RPDYKELKFF KREYPDIPMI ALTATASEQV
     851  RMDIIHNLEL KEPVFLKQSF NRTNLYYEVN KKTKNTIFEI CDAVKSRFKN
     901  QTGIIYCHSK KSCEQTSAQM QRNGIKCAYY HAGMEPDERL SVQKAWQADE
     951  IQVICATVAF GMGIDKPDVR FVYHFTVPRT LEGYYQETGR AGRDGNYSYC
    1001  ITYFSFRDIR TMQTMIQKDK NLDRENKEKH LNKLQQVMAY CDNVTDCRRK
    1051  LVLSYFNEDF DSKLCHKNCD NCRNSANVIN EERDVTEPAK KIVKLVESIQ
    1101  NERVTIIYCQ DVFKGSRSSK IVQANHDTLE EHGIGKSMQK SEIERIFFHL
    1151  ITIRVLQEYS IMNNSGFASS YVKVGPNAKK LLTGKMEIKM QFTISAPNSR
    1201  PSTSSSFQAN EDNIPVIAQK STTIGGNVAA NPPRFISAKE HLRSYTYGGS
    1251  TMGSSHPITL KNTSDLRSTQ ELNNLRMTYE RLRELSLNLG NRMVPPVGNF
    1301  MPDSILKKMA AILPMNDSAF ATLGTVEDKY RRRFKYFKAT IADLSKKRSS
    1351  EDHEKYDTIL NDEFVNRAAA SSNGIAQSTG TKSKFFGANL NEAKENEQII
    1401  NQIRQSQLPK NTTSSKSGTR SISKSSKKSA NGRRGFRNYR GHYRGRK*

external links for Sgs1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Sgs1p

1)	Sternglanz, R. (1993) Personal Communication, Mortimer Map Edition 12
2)	Sun H, et al. (1999) The Saccharomyces cerevisiae Sgs1 helicase efficiently unwinds G-G paired DNAs. Nucleic Acids Res 27(9):1978-84
3)	Huber MD, et al. (2002) G4 DNA unwinding by BLM and Sgs1p: substrate specificity and substrate-specific inhibition. Nucleic Acids Res 30(18):3954-61
4)	Versini G, et al. (2003) The yeast Sgs1 helicase is differentially required for genomic and ribosomal DNA replication. EMBO J 22(8):1939-49
5)	Rockmill B, et al. (2003) The Sgs1 helicase regulates chromosome synapsis and meiotic crossing over. Curr Biol 13(22):1954-62
6)	Oh SD, et al. (2008) RecQ helicase, Sgs1, and XPF family endonuclease, Mus81-Mms4, resolve aberrant joint molecules during meiotic recombination. Mol Cell 31(3):324-36
7)	Jessop L and Lichten M (2008) Mus81/Mms4 endonuclease and Sgs1 helicase collaborate to ensure proper recombination intermediate metabolism during meiosis. Mol Cell 31(3):313-23
8)	Marrakchi R, et al. (2011) The isomerase Rrd1 mediates rapid loss of the Sgs1 helicase in response to rapamycin. Biochem Cell Biol 89(3):332-40
9)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76