HOT1/YMR172W Protein Information Help

Standard Name Hot1p 1
Systematic Name Ymr172wp
ORF Classification Verified
Description Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p (1, 2, 3)
Name Description High-Osmolarity-induced Transcription 1
Experimental Data
Molecules/cell 149 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 719
Molecular Weight (Da) 79,415
Isoelectric Point (pI) 8.2

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Hot1p (InterPro)
Transmembrane Domains There are 1 total predicted transmembrane domains (TMHMM)
Signal Peptide(s) There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions There are 30 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052F01 | Q03213
MIPS: YMR172W
NCBI: 2497172 | 6323824 | 854442 | NP_013895.1
GenBank/EMBL/DDBJ: DAA10068.1 | Z49808
Amino Acid Sequence (or in FASTA format)
 
       1  MSGMGIAILC IVRTKIYRIT ISFDYSTLMS PFFLFLMMPT TLKDGYRMNS
      51  QVNEDAIGIN LDLSLPTHIS PTTGSESASG SNASTLRNDG NALDGGLLRT
     101  SAAISAPTGT SQPTETIGEK LSNEERVNSN VSASNSTTAG TGRMLSQSLT
     151  NDSPSNEIST DQLKIFQRMD EMSARMIEME ESFNKLSNKI AEQNTMVLNL
     201  KQDNYKVMNK LNILLKLVAQ PSARPSTNNA QNKLAIELLN SISAVSSAYL
     251  QKMQNNGSGR QHTADLCTGD SNTHSGINQH RTTNGTIDVN TNTAQLNNQF
     301  SNALNTILPD QQHNRNNVSQ NINQSLPNRQ LGPVINTQAN QNQSQVLIHN
     351  TNTHQQVNRS PISFPNASTD KPFKLNPNGI KRRRRNTQSN NNASTNDHAS
     401  AAQKPISALS PLTNSHNSTT SMNYTNSSIH SGVTSASNSF HDLNSLNNFG
     451  TTTALSLPSL ALDNASFPPN QNVIPPIINN TQQPLSFSQL INQDSTTSEL
     501  LPSGKSGVNT NIVNRNRAST LPSYPKPMTV KSNVDDDGYQ EDDDDDGDDE
     551  GDGRDNEEDS TAEEDEVDDE IETDMKNASI NKRRRSLHHK KSNSLNGRRK
     601  LHGESATKPN INSDLHYRIL KAPTDVKTIW EEYDTGIRGK PSIKHLEAKY
     651  GNKWRLNKNK KTFSRRKRLY KFILNGMERG KTAQEMIETL ENKRLYKDDE
     701  DGEVKKRTIG WLQESLAGI*

external links for Hot1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Hot1p
1) Rep M, et al.  (1999) Osmotic stress-induced gene expression in Saccharomyces cerevisiae requires Msn1p and the novel nuclear factor Hot1p. Mol Cell Biol 19(8):5474-85
2) Rep M, et al.  (2000) The transcriptional response of Saccharomyces cerevisiae to osmotic shock. Hot1p and Msn2p/Msn4p are required for the induction of subsets of high osmolarity glycerol pathway-dependent genes. J Biol Chem 275(12):8290-300
3) Alepuz PM, et al.  (2003) Osmostress-induced transcription by Hot1 depends on a Hog1-mediated recruitment of the RNA Pol II. EMBO J 22(10):2433-42
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41