CEP3/YMR168C Protein Information Help

Standard Name Cep3p 1
Systematic Name Ymr168cp
Alias Cbf3p 2 , Csl1p 3
ORF Classification Verified
Description Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain (1, 4, 5) Also known as: CBF3B 2
Name Description CEntromere Protein 6
Experimental Data
Molecules/cell 1900 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 608
Molecular Weight (Da) 71,357
Isoelectric Point (pI) 6.26

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Cep3p (InterPro)
Physical Interactions There are 45 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000127153 | P40969
MIPS: YMR168C
NCBI: 160286017 | 163931085 | 575395 | 595483 | 6323820 | 729057 | 825573 | NP_013891.1 | NM_001182672.1
GenBank/EMBL/DDBJ: DAA10064.1 | U12339 | X81396 | Z49705
Amino Acid Sequence (or in FASTA format)
 
       1  MFNRTTQLKS KHPCSVCTRR KVKCDRMIPC GNCRKRGQDS ECMKSTKLIT
      51  ASSSKEYLPD LLLFWQNYEY WITNIGLYKT KQRDLTRTPA NLDTDTEECM
     101  FWMNYLQKDQ SFQLMNFAME NLGALYFGSI GDISELYLRV EQYWDRRADK
     151  NHSVDGKYWD ALIWSVFTMC IYYMPVEKLA EIFSVYPLHE YLGSNKRLNW
     201  EDGMQLVMCQ NFARCSLFQL KQCDFMAHPD IRLVQAYLIL ATTTFPYDEP
     251  LLANSLLTQC IHTFKNFHVD DFRPLLNDDP VESIAKVTLG RIFYRLCGCD
     301  YLQSGPRKPI ALHTEVSSLL QHAAYLQDLP NVDVYREENS TEVLYWKIIS
     351  LDRDLDQYLN KSSKPPLKTL DAIRRELDIF QYKVDSLEED FRSNNSRFQK
     401  FIALFQISTV SWKLFKMYLI YYDTADSLLK VIHYSKVIIS LIVNNFHAKS
     451  EFFNRHPMVM QTITRVVSFI SFYQIFVESA AVKQLLVDLT ELTANLPTIF
     501  GSKLDKLVYL TERLSKLKLL WDKVQLLDSG DSFYHPVFKI LQNDIKIIEL
     551  KNDEMFSLIK GLGSLVPLNK LRQESLLEEE DENNTEPSDF RTIVEEFQSE
     601  YNISDILS*

external links for Cep3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Cep3p
1) Strunnikov AV, et al.  (1995) CEP3 encodes a centromere protein of Saccharomyces cerevisiae. J Cell Biol 128(5):749-60
2) Lechner J and Carbon J  (1991) A 240 kd multisubunit protein complex, CBF3, is a major component of the budding yeast centromere. Cell 64(4):717-25
3) Baker RE, et al.  (1998) Mutations synthetically lethal with cep1 target S. cerevisiae kinetochore components. Genetics 149(1):73-85
4) Espelin CW, et al.  (1997) Probing the architecture of a simple kinetochore using DNA-protein crosslinking. J Cell Biol 139(6):1383-96
5) Lechner J  (1994) A zinc finger protein, essential for chromosome segregation, constitutes a putative DNA binding subunit of the Saccharomyces cerevisiae kinetochore complex, Cbf3. EMBO J 13(21):5203-11
6) Baker RE and Masison DC  (1990) Isolation of the gene encoding the Saccharomyces cerevisiae centromere-binding protein CP1. Mol Cell Biol 10(6):2458-67
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41