DNF3/YMR162C Protein Information Help

Standard Name Dnf3p 1
Systematic Name Ymr162cp
ORF Classification Verified
Description Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; localizes to the trans-Golgi network; likely involved in protein transport; type 4 P-type ATPase (1, 2, 3)
Name Description Drs2 Neo1 Family 1
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,656
Molecular Weight (Da) 188,318
Isoelectric Point (pI) 6.74

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Dnf3p (InterPro)
Transmembrane Domains There are 8 total predicted transmembrane domains (TMHMM)
Physical Interactions There are 8 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000126209 | Q12674
MIPS: YMR162C
NCBI: 2493011 | 6323814 | 825567 | NP_013885.1
GenBank/EMBL/DDBJ: DAA10058.1 | Z49705
External Classifications EC: 3.6.3.1 [Phospholipid-translocating ATPase] TC: 3.A.3.8 [The Aminophospholipid-ATPase (PLA) Family]
Amino Acid Sequence (or in FASTA format)
 
       1  MGIADGQRRR SSSLRTQMFN KHLYDKYRGR TDDEIELEDI NESKTFSGSD
      51  NNDKDDRDET SGNYAAEEDY EMEEYGSPDV SYSIITKILD TILDRRRTFH
     101  SKDGRHIPII LDHNAIEYKQ AATKRDGHLI DERFNKPYCD NRITSSRYTF
     151  YSFLPRQLYA QFSKLANTYF FIVAVLQMIP GWSTTGTYTT IIPLCVFMGI
     201  SMTREAWDDF RRHRLDKEEN NKPVGVLVKD GNNDAQEVYT LPSSVVSSTA
     251  YLTKSAAAEN NPPLNDDRNS SQGHFLDTHF NNFELLKNKY NVHIHQKKWE
     301  KLRVGDFVLL TQDDWVPADL LLLTCDGENS ECFVETMALD GETNLKSKQP
     351  HPELNKLTKA ASGLANINAQ VTVEDPNIDL YNFEGNLELK NHRNDTIMKY
     401  PLGPDNVIYR GSILRNTQNV VGMVIFSGEE TKIRMNALKN PRTKAPKLQR
     451  KINMIIVFMV FVVATISLFS YLGHVLHKKK YIDQNKAWYL FQADAGVAPT
     501  IMSFIIMYNT VIPLSLYVTM EIIKVVQSKM MEWDIDMYHA ETNTPCESRT
     551  ATILEELGQV SYIFSDKTGT LTDNKMIFRK FSLCGSSWLH NVDLGNSEDN
     601  FEDNRDNTNS LRLPPKAHNG SSIDVVSIGD QNVLDRLGFS DAPIEKGHRP
     651  SLDNFPKSRN SIEYKGNSSA IYTGRPSMRS LFGKDNSHLS KQASVISPSE
     701  TFSENIKSSF DLIQFIQRYP TALFSQKAKF FFLSLALCHS CLPKKTHNES
     751  IGEDSIEYQS SSPDELALVT AARDLGYIVL NRNAQILTIK TFPDGFDGEA
     801  KLENYEILNY IDFNSQRKRM SVLVRMPNQP NQVLLICKGA DNVIMERLHD
     851  RELAAKKMAD ICTSTKERKD AEAELVLQQR KSLERMVDEE AMARTSLRNS
     901  LSSVPRASLS LQAVRKSLSM KNSRTRDPEK QIDSIDQFLE TVKKSDQEIG
     951  SVVNKSRKSL HKQQIEKYGP RISIDGTHFP NNNVPIDTRK EGLQHDYDTE
    1001  ILEHIGSDEL ILNEEYVIER TLQAIDEFST EGLRTLVYAY KWIDIGQYEN
    1051  WNKRYHQAKT SLTDRKIKVD EAGAEIEDGL NLLGVTAIED KLQDGVSEAI
    1101  EKIRRAGIKM WMLTGDKRET AINIGYSCML IKDYSTVVIL TTTDENIISK
    1151  MNAVSQEVDS GNIAHCVVVI DGATMAMFEG NPTYMSVFVE LCTKTDSVIC
    1201  CRASPSQKAL MVSNIRNTDP NLVTLAIGDG ANDIAMIQSA DIGVGIAGKE
    1251  GLQASRVSDY SIGQFRFLLK LLFVHGRYNY IRTSKFMLCT FYKEITFYFT
    1301  QLIYQRYTMF SGSSLYEPWS LSMFNTLFTS LPVLCIGMFE KDLKPMTLLT
    1351  VPELYSYGRL SQGFNWLIFM EWVILATTNS LIITFLNVVM WGMSSLSDNT
    1401  MYPLGLINFT AIVALINVKS QFVEMHNRNW LAFTSVVLSC GGWLVWCCAL
    1451  PILNNTDQIY DVAYGFYNHF GKDITFWCTS LVLALLPITL DIVYKTFKVM
    1501  IWPSDSDIFA ELEQKSDIRK KLELGAYSEM RQGWTWDKDP STFTRYTDKV
    1551  LSRPRTNSRA SAKTHNSSIY SMSNGNVDHS SKKNFFGNSS KKSSERYEVL
    1601  PSGKLIKRPS LKTQSSKDSI GGNITTKLTK KLKLPSRNVE DEDVNQIIQA
    1651  RLKDLE*

external links for Dnf3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome TCDB
YGOB GeneMANIA YeastRC Structure Prediction (Seattle)
YOGY


References cited on this page View Complete Literature Guide for Dnf3p
1) Hua Z, et al.  (2002) An essential subfamily of Drs2p-related P-type ATPases is required for protein trafficking between Golgi complex and endosomal/vacuolar system. Mol Biol Cell 13(9):3162-77
2) Alder-Baerens N, et al.  (2006) Loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles. Mol Biol Cell 17(4):1632-42
3) Catty P, et al.  (1997) The complete inventory of the yeast Saccharomyces cerevisiae P-type transport ATPases. FEBS Lett 409(3):325-32