HLJ1/YMR161W Protein Information

Standard Name	Hlj1p 1
Systematic Name	Ymr161wp
ORF Classification	Verified
Description	Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ (2, 3, 4, 5)
Name Description	HomoLogous to E. coli dnaJ protein

Experimental Data
Molecules/cell	1840 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	224
Molecular Weight (Da)	25,008
Isoelectric Point (pI)	10.07

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Hlj1p (InterPro)
Transmembrane Domains	There are 1 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 2 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012C941 \| P48353 MIPS: YMR161W NCBI: 1346294 \| 6323813 \| 825566 \| 972936 \| NP_013884.1 GenBank/EMBL/DDBJ: DAA10057.1 \| U19358 \| Z49705

Amino Acid Sequence (or in FASTA format)
1 MSFTEDQEKI ALEILSKDKH EFYEILKVDR KATDSEIKKA YRKLAIKLHP 51 DKNSHPKAGE AFKVINRAFE VLSNEEKRSI YDRIGRDPDD RQMPSRGAAS 101 GFRGSAGGSP MGGGFEDMFF NSRFGGQRAG PPEDIFDFLF NAGGSPFGAS 151 PFGPSASTFS FGGPGGFRVY TNNRGGSPFM RQQPRSRQQQ QQAEENAVNS 201 QLKNMLVLFI IFIVLPMIKD YLFS*

Amino Acid Sequence (or in FASTA format)

       1  MSFTEDQEKI ALEILSKDKH EFYEILKVDR KATDSEIKKA YRKLAIKLHP
      51  DKNSHPKAGE AFKVINRAFE VLSNEEKRSI YDRIGRDPDD RQMPSRGAAS
     101  GFRGSAGGSP MGGGFEDMFF NSRFGGQRAG PPEDIFDFLF NAGGSPFGAS
     151  PFGPSASTFS FGGPGGFRVY TNNRGGSPFM RQQPRSRQQQ QQAEENAVNS
     201  QLKNMLVLFI IFIVLPMIKD YLFS*

external links for Hlj1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	GeneMANIA

References cited on this page View Complete Literature Guide for Hlj1p

1)	Stepanek, P., et al. (1995) HLJ1, a Saccharomyces cerevisiae homolog of Escherichia coli dnaJ with a high-copy lethal phenotype. Unpublished
2)	Beilharz T, et al. (2003) Bipartite signals mediate subcellular targeting of tail-anchored membrane proteins in Saccharomyces cerevisiae. J Biol Chem 278(10):8219-23
3)	Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
4)	Huyer G, et al. (2004) Distinct machinery is required in Saccharomyces cerevisiae for the endoplasmic reticulum-associated degradation of a multispanning membrane protein and a soluble luminal protein. J Biol Chem 279(37):38369-78
5)	Youker RT, et al. (2004) Distinct roles for the Hsp40 and Hsp90 molecular chaperones during cystic fibrosis transmembrane conductance regulator degradation in yeast. Mol Biol Cell 15(11):4787-97
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41