ATG16/YMR159C Protein Information Help

Standard Name Atg16p 1
Systematic Name Ymr159cp
Alias Sap18p 2 , Cvt11p 3 , Apg16p 4 , Apg15p 5
ORF Classification Verified
Description Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress (4, 6, 7, 8, 9)
Name Description AuTophaGy related 1
Experimental Data
Molecules/cell 573 10
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 150
Molecular Weight (Da) 17,222
Isoelectric Point (pI) 6.31

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Atg16p (InterPro)
Physical Interactions There are 31 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013B8EA | Q03818
MIPS: YMR159C
NCBI: 122920045 | 122920047 | 122920049 | 122920051 | 122920054 | 2497167 | 262367845 | 262367846 | 262367847 | 262367848 | 262367849 | 262367850 | 262367851 | 262367852 | 433286618 | 6323811 | 825564 | NP_013882.1
GenBank/EMBL/DDBJ: DAA10055.1 | Z49705
Amino Acid Sequence (or in FASTA format)
 
       1  MGNFIITERK KAKEERSNPQ TDSMDDLLIR RLTDRNDKEA HLNELFQDNS
      51  GAIGGNIVSH DDALLNTLAI LQKELKSKEQ EIRRLKEVIA LKNKNTERLN
     101  DELISGTIEN NVLQQKLSDL KKEHSQLVAR WLKKTEKETE AMNSEIDGTK
     151  *

external links for Atg16p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
YGOB BioPIXIE MIPS YeastGFP
YOGY CYC2008 (complexes) Pfam domains YeastRC Public Image Repository

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Atg16p
1) Klionsky DJ, et al.  (2003) A unified nomenclature for yeast autophagy-related genes. Dev Cell 5(4):539-45
2) Zhang Y, et al.  (1997) Histone deacetylases and SAP18, a novel polypeptide, are components of a human Sin3 complex. Cell 89(3):357-64
3) Harding TM, et al.  (1996) Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway. J Biol Chem 271(30):17621-4
4) Mizushima N, et al.  (1999) Apg16p is required for the function of the Apg12p-Apg5p conjugate in the yeast autophagy pathway. EMBO J 18(14):3888-96
5) Tsukada M and Ohsumi Y  (1993) Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae. FEBS Lett 333(1-2):169-74
6) Suzuki K, et al.  (2001) The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation. EMBO J 20(21):5971-81
7) Kuma A, et al.  (2002) Formation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast. J Biol Chem 277(21):18619-25
8) Meijer WH, et al.  (2007) ATG genes involved in non-selective autophagy are conserved from yeast to man, but the selective Cvt and pexophagy pathways also require organism-specific genes. Autophagy 3(2):106-16
9) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
10) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41