ECM16/YMR128W Protein Information

Standard Name	Ecm16p 1
Systematic Name	Ymr128wp
Alias	Dhr1p
ORF Classification	Verified
Description	Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis (2, 3, 4)
Name Description	ExtraCellular Mutant 1

Experimental Data
Molecules/cell	2000 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,267
Molecular Weight (Da)	144,953
Isoelectric Point (pI)	6.28

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ecm16p (InterPro)
Physical Interactions	There are 114 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052F3B \| Q04217 MIPS: YMR128W NCBI: 2500542 \| 6323776 \| 728667 \| NP_013847.1 GenBank/EMBL/DDBJ: DAA10025.1 \| Z48622
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides]

Amino Acid Sequence (or in FASTA format)
1 MGTYRKRFNE KARSGHMAKL KELKRIRNKQ FTRQDENDER VENPDSAPAE 51 SSTTEPNANA EILEPLTEEE KKMKKRKLQE LFTPKESKVS RLKKKRLDKF 101 IEHQLKREER KTIIGKLQDY KIDTSLLTSS KRLGEGRQTK KEEFKEALSL 151 ERQGRGNEQT NEILYEEYEP KVWDEYGEGG SSEDDDGEDD FEASFGSMPK 201 PTDNEEKKSS GFIDHRPAKF GGSGLSFGFS NIKVINKESK TPKKKYNWRQ 251 RVEMEELKKH GKEDEMDFDT TSEDDDEEED QEEEDKMHPS ENPLEEVESA 301 DSETGSEKFD QNDVANEFKD WANQEIKKLE GRDQELVTPT LNIDYKPIIR 351 KEDLDDGLQE AYVPINENST RKAFYVEVSR SDEIQKARIQ LPVFGEEHKI 401 MEAIHHNDVV IICGETGSGK TTQVPQFLYE AGFGAEDSPD YPGMVGITQP 451 RRVAAVSMAE RVANELGDHG HKVGYQIRFD STAKEDTKVK FMTDGVLLRE 501 MMHDFKLTKY SSIIIDEAHE RNINTDILIG MLSRCVRLRA KLHKENPIEH 551 KKLKLIIMSA TLRVSDFSEN KTLFPIAPPV LQVDARQFPV SIHFNRRTAF 601 NYTDEAFRKT CKIHQKLPPG AILVFLTGQQ EITHMVKRLR KEFPFKKNSK 651 YNKDLETPVS KMGINSKTTD LEAEDIDFSV QVIDQDKFKS AIRYEEDEGN 701 SGNGEDEEDE EEEGFEEVLT EGQTANDPLY VLPLYSLLPT KEQMRVFQKP 751 PQGSRLCIVA TNVAETSLTI PGVRYVVDSG RSKERKYNES NGVQSFEVGW 801 VSKASANQRS GRAGRTGPGH CYRLYSSAVF EHDFEQFSKP EILRMPVESI 851 VLQMKSMAIH NIINFPFPTP PDRVALSKAI QLLQYLGALD NKEMITEDGK 901 KMSLFPLSPR FSKMLLVSDE KACLPYIVAI VSALSVGDPF INEFELGINE 951 ISRKPNPDEN LDDKIREHDE STPGMDPELK KELRSKFYKS RSQFSKLDKF 1001 SDVFRLLSVV SAMDYVPKEQ KEIFMKKNFL RGKLMEEIVK LRKQLMYIIK 1051 SNTSKENIAV VIRNEDLKSD IPSVIQIKLL KQMICAGFVD HVAVRADVLF 1101 PDDAKITNRT SIINIPYIPV LATRTPNIED CFVYIHPTSI LNNLGEMPPK 1151 YMLYYSLHLG GNNKTRMNTL CDIASTPLAN IARKGLLLTY SKPLTGQGLK 1201 TVNLSPTERY CYVVPRFGST VDNDLKIGWD LNPIAVHQKK QKGQWTVIKF 1251 ITRKGFQTIT GEEKEKK*

Amino Acid Sequence (or in FASTA format)

       1  MGTYRKRFNE KARSGHMAKL KELKRIRNKQ FTRQDENDER VENPDSAPAE
      51  SSTTEPNANA EILEPLTEEE KKMKKRKLQE LFTPKESKVS RLKKKRLDKF
     101  IEHQLKREER KTIIGKLQDY KIDTSLLTSS KRLGEGRQTK KEEFKEALSL
     151  ERQGRGNEQT NEILYEEYEP KVWDEYGEGG SSEDDDGEDD FEASFGSMPK
     201  PTDNEEKKSS GFIDHRPAKF GGSGLSFGFS NIKVINKESK TPKKKYNWRQ
     251  RVEMEELKKH GKEDEMDFDT TSEDDDEEED QEEEDKMHPS ENPLEEVESA
     301  DSETGSEKFD QNDVANEFKD WANQEIKKLE GRDQELVTPT LNIDYKPIIR
     351  KEDLDDGLQE AYVPINENST RKAFYVEVSR SDEIQKARIQ LPVFGEEHKI
     401  MEAIHHNDVV IICGETGSGK TTQVPQFLYE AGFGAEDSPD YPGMVGITQP
     451  RRVAAVSMAE RVANELGDHG HKVGYQIRFD STAKEDTKVK FMTDGVLLRE
     501  MMHDFKLTKY SSIIIDEAHE RNINTDILIG MLSRCVRLRA KLHKENPIEH
     551  KKLKLIIMSA TLRVSDFSEN KTLFPIAPPV LQVDARQFPV SIHFNRRTAF
     601  NYTDEAFRKT CKIHQKLPPG AILVFLTGQQ EITHMVKRLR KEFPFKKNSK
     651  YNKDLETPVS KMGINSKTTD LEAEDIDFSV QVIDQDKFKS AIRYEEDEGN
     701  SGNGEDEEDE EEEGFEEVLT EGQTANDPLY VLPLYSLLPT KEQMRVFQKP
     751  PQGSRLCIVA TNVAETSLTI PGVRYVVDSG RSKERKYNES NGVQSFEVGW
     801  VSKASANQRS GRAGRTGPGH CYRLYSSAVF EHDFEQFSKP EILRMPVESI
     851  VLQMKSMAIH NIINFPFPTP PDRVALSKAI QLLQYLGALD NKEMITEDGK
     901  KMSLFPLSPR FSKMLLVSDE KACLPYIVAI VSALSVGDPF INEFELGINE
     951  ISRKPNPDEN LDDKIREHDE STPGMDPELK KELRSKFYKS RSQFSKLDKF
    1001  SDVFRLLSVV SAMDYVPKEQ KEIFMKKNFL RGKLMEEIVK LRKQLMYIIK
    1051  SNTSKENIAV VIRNEDLKSD IPSVIQIKLL KQMICAGFVD HVAVRADVLF
    1101  PDDAKITNRT SIINIPYIPV LATRTPNIED CFVYIHPTSI LNNLGEMPPK
    1151  YMLYYSLHLG GNNKTRMNTL CDIASTPLAN IARKGLLLTY SKPLTGQGLK
    1201  TVNLSPTERY CYVVPRFGST VDNDLKIGWD LNPIAVHQKK QKGQWTVIKF
    1251  ITRKGFQTIT GEEKEKK*

external links for Ecm16p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
YGOB	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Ecm16p

1)	Lussier M, et al. (1997) Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147(2):435-50
2)	Shiratori A, et al. (1999) Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and Northern analysis. Yeast 15(3):219-53
3)	Colley A, et al. (2000) Dhr1p, a putative DEAH-box RNA helicase, is associated with the box C+D snoRNP U3. Mol Cell Biol 20(19):7238-46
4)	Dragon F, et al. (2002) A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis. Nature 417(6892):967-70
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41