ASC1/YMR116C Protein Information

Standard Name	Asc1p 1
Systematic Name	Ymr116cp
Alias	Cpc2p 2 , Nad1p 3
ORF Classification	Verified
Description	G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation (1, 2, 4, 5, 6)
Name Description	Absence of growth Suppressor of Cyp1 1

Experimental Data
Molecules/cell	333000 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	319
Molecular Weight (Da)	34,805
Isoelectric Point (pI)	6.17

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Asc1p (InterPro)
Physical Interactions	There are 99 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052E47 \| P38011 MIPS: YMR116C NCBI: 1730231 \| 223674073 \| 223674074 \| 223674075 \| 223674076 \| 315113467 \| 315113495 \| 339717630 \| 339717631 \| 339717632 \| 339717633 \| 339717634 \| 339717635 \| 55670021 \| 6323763 \| 817875 \| 817876 \| NP_013834.1 GenBank/EMBL/DDBJ: DAA10013.1 \| Z49702

Amino Acid Sequence (or in FASTA format)
1 MASNEVLVLR GTLEGHNGWV TSLATSAGQP NLLLSASRDK TLISWKLTGD 51 DQKFGVPVRS FKGHSHIVQD CTLTADGAYA LSASWDKTLR LWDVATGETY 101 QRFVGHKSDV MSVDIDKKAS MIISGSRDKT IKVWTIKGQC LATLLGHNDW 151 VSQVRVVPNE KADDDSVTII SAGNDKMVKA WNLNQFQIEA DFIGHNSNIN 201 TLTASPDGTL IASAGKDGEI MLWNLAAKKA MYTLSAQDEV FSLAFSPNRY 251 WLAAATATGI KVFSLDPQYL VDDLRPEFAG YSKAAEPHAV SLAWSADGQT 301 LFAGYTDNVI RVWQVMTAN*

Amino Acid Sequence (or in FASTA format)

       1  MASNEVLVLR GTLEGHNGWV TSLATSAGQP NLLLSASRDK TLISWKLTGD
      51  DQKFGVPVRS FKGHSHIVQD CTLTADGAYA LSASWDKTLR LWDVATGETY
     101  QRFVGHKSDV MSVDIDKKAS MIISGSRDKT IKVWTIKGQC LATLLGHNDW
     151  VSQVRVVPNE KADDDSVTII SAGNDKMVKA WNLNQFQIEA DFIGHNSNIN
     201  TLTASPDGTL IASAGKDGEI MLWNLAAKKA MYTLSAQDEV FSLAFSPNRY
     251  WLAAATATGI KVFSLDPQYL VDDLRPEFAG YSKAAEPHAV SLAWSADGQT
     301  LFAGYTDNVI RVWQVMTAN*

external links for Asc1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Asc1p

1)	Chantrel Y, et al. (1998) The transcriptional regulator Hap1p (Cyp1p) is essential for anaerobic or heme-deficient growth of Saccharomyces cerevisiae: Genetic and molecular characterization of an extragenic suppressor that encodes a WD repeat protein. Genetics 148(2):559-69
2)	Hoffmann B, et al. (1999) The WD protein Cpc2p is required for repression of Gcn4 protein activity in yeast in the absence of amino-acid starvation. Mol Microbiol 31(3):807-22
3)	Kuroha K, et al. (2010) Receptor for activated C kinase 1 stimulates nascent polypeptide-dependent translation arrest. EMBO Rep 11(12):956-61
4)	Gerbasi VR, et al. (2004) Yeast Asc1p and mammalian RACK1 are functionally orthologous core 40S ribosomal proteins that repress gene expression. Mol Cell Biol 24(18):8276-87
5)	Zeller CE, et al. (2007) The RACK1 ortholog Asc1 functions as a G-protein beta subunit coupled to glucose responsiveness in yeast. J Biol Chem 282(34):25168-76
6)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41