YKU80/YMR106C Protein Information

Standard Name	Yku80p 1
Systematic Name	Ymr106cp
Alias	Hdf2p 2
ORF Classification	Verified
Description	Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair (1, 3, 4, 5, 6, 7)
Name Description	Yeast KU protein 1

Experimental Data
Molecules/cell	358 8

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	629
Molecular Weight (Da)	71,240
Isoelectric Point (pI)	4.99

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Yku80p (InterPro)
Physical Interactions	There are 101 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000034135 \| Q04437 MIPS: YMR106C NCBI: 112362655 \| 112362657 \| 112362659 \| 112362661 \| 112362663 \| 112362665 \| 112362667 \| 112362669 \| 112362671 \| 112362673 \| 112362675 \| 112362677 \| 112362679 \| 2498435 \| 6323753 \| 817864 \| NP_013824.1 \| NM_001182606.1 GenBank/EMBL/DDBJ: DAA10003.1 \| AM296333 \| AM296334 \| AM296335 \| AM296336 \| AM296337 \| AM296338 \| AM296339 \| AM296340 \| AM296341 \| AM296342 \| AM296343 \| AM296344 \| AM296345 \| Z49702
External Classifications	EC: 3.6.4.12 [DNA helicase]

Amino Acid Sequence (or in FASTA format)
1 MSSESTTFIV DVSPSMMKNN NVSKSMAYLE YTLLNKSKKS RKTDWISCYL 51 ANCPVSENSQ EIPNVFQIQS FLAPVTTTAT IGFIKRLKQY CDQHSHDSSN 101 EGLQSMIQCL LVVSLDIKQQ FQARKILKQI VVFTDNLDDL DITDEEIDLL 151 TEELSTRIIL IDCGKDTQEE RKKSNWLKLV EAIPNSRIYN MNELLVEITS 201 PATSVVKPVR VFSGELRLGA DILSTQTSNP SGSMQDENCL CIKVEAFPAT 251 KAVSGLNRKT AVEVEDSQKK ERYVGVKSII EYEIHNEGNK KNVSEDDQSG 301 SSYIPVTISK DSVTKAYRYG ADYVVLPSVL VDQTVYESFP GLDLRGFLNR 351 EALPRYFLTS ESSFITADTR LGCQSDLMAF SALVDVMLEN RKIAVARYVS 401 KKDSEVNMCA LCPVLIEHSN INSEKKFVKS LTLCRLPFAE DERVTDFPKL 451 LDRTTTSGVP LKKETDGHQI DELMEQFVDS MDTDELPEIP LGNYYQPIGE 501 VTTDTTLPLP SLNKDQEENK KDPLRIPTVF VYRQQQVLLE WIHQLMINDS 551 REFEIPELPD SLKNKISPYT HKKFDSTKLV EVLGIKKVDK LKLDSELKTE 601 LEREKIPDLE TLLKRGEQHS RGSPNNSNN*

Amino Acid Sequence (or in FASTA format)

       1  MSSESTTFIV DVSPSMMKNN NVSKSMAYLE YTLLNKSKKS RKTDWISCYL
      51  ANCPVSENSQ EIPNVFQIQS FLAPVTTTAT IGFIKRLKQY CDQHSHDSSN
     101  EGLQSMIQCL LVVSLDIKQQ FQARKILKQI VVFTDNLDDL DITDEEIDLL
     151  TEELSTRIIL IDCGKDTQEE RKKSNWLKLV EAIPNSRIYN MNELLVEITS
     201  PATSVVKPVR VFSGELRLGA DILSTQTSNP SGSMQDENCL CIKVEAFPAT
     251  KAVSGLNRKT AVEVEDSQKK ERYVGVKSII EYEIHNEGNK KNVSEDDQSG
     301  SSYIPVTISK DSVTKAYRYG ADYVVLPSVL VDQTVYESFP GLDLRGFLNR
     351  EALPRYFLTS ESSFITADTR LGCQSDLMAF SALVDVMLEN RKIAVARYVS
     401  KKDSEVNMCA LCPVLIEHSN INSEKKFVKS LTLCRLPFAE DERVTDFPKL
     451  LDRTTTSGVP LKKETDGHQI DELMEQFVDS MDTDELPEIP LGNYYQPIGE
     501  VTTDTTLPLP SLNKDQEENK KDPLRIPTVF VYRQQQVLLE WIHQLMINDS
     551  REFEIPELPD SLKNKISPYT HKKFDSTKLV EVLGIKKVDK LKLDSELKTE
     601  LEREKIPDLE TLLKRGEQHS RGSPNNSNN*

external links for Yku80p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Yku80p

1)	Boulton SJ and Jackson SP (1996) Identification of a Saccharomyces cerevisiae Ku80 homologue: roles in DNA double strand break rejoining and in telomeric maintenance. Nucleic Acids Res 24(23):4639-48
2)	Feldmann H, et al. (1996) HDF2, the second subunit of the Ku homologue from Saccharomyces cerevisiae. J Biol Chem 271(44):27765-9
3)	Martin SG, et al. (1999) Relocalization of telomeric Ku and SIR proteins in response to DNA strand breaks in yeast. Cell 97(5):621-33
4)	Milne GT, et al. (1996) Mutations in two Ku homologs define a DNA end-joining repair pathway in Saccharomyces cerevisiae. Mol Cell Biol 16(8):4189-98
5)	Boulton SJ and Jackson SP (1998) Components of the Ku-dependent non-homologous end-joining pathway are involved in telomeric length maintenance and telomeric silencing. EMBO J 17(6):1819-28
6)	Laroche T, et al. (1998) Mutation of yeast Ku genes disrupts the subnuclear organization of telomeres. Curr Biol 8(11):653-6
7)	Bertuch AA and Lundblad V (2003) The Ku heterodimer performs separable activities at double-strand breaks and chromosome termini. Mol Cell Biol 23(22):8202-15
8)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41