UBX4/YMR067C Protein Information

Standard Name	Ubx4p 1
Systematic Name	Ymr067cp
Alias	Cui1p 2
ORF Classification	Verified
Description	UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress (1, 3, 4)
Name Description	UBiquitin regulatory X 1

Experimental Data
Molecules/cell	3570 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	416
Molecular Weight (Da)	46,964
Isoelectric Point (pI)	9.54

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Ubx4p (InterPro)
Physical Interactions	There are 15 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013BA31 \| P54730 MIPS: YMR067C NCBI: 1730608 \| 6323712 \| 763014 \| NP_013783.1 GenBank/EMBL/DDBJ: DAA09965.1 \| Z48952

Amino Acid Sequence (or in FASTA format)
1 MPMVTVKYNF QLFKCKVSLN STLNDVLHQS IQFFQLHTSS NDWSLIHLDK 51 PVPLDLPWRL LNLPTGVNLE LSKSSNFPVA NKTNREDIPF NTIKIRFQIP 101 GRDSVVKEMP SDQPIAPILR QMSGAAGDDF KIQVFSKIIE FKTIKDENLT 151 LENLGIQEPS SVRLIFNNTS HSEGISANSA IHPKQTPPTM TNPETVASLP 201 PHELHKPSVF LPSDEPLAVI KDQIEDEEDY ELTVEQAKKY QKMLSSKAGT 251 LGGPILTKRL REQSANNLPK KNKAISECLL RVKFPDRSHI QIAFKPNEDM 301 RTVYNVVSQF LIDENMPFTL NQSHPFKPLA KDDKKLLDDL EFGSKTMLLF 351 ETNSNSNGPL IKAHLLEDAQ KITHETRTTP SVNTINKSNP QGPSDNATSI 401 KKTLNRVPKW MKLSKK*

Amino Acid Sequence (or in FASTA format)

       1  MPMVTVKYNF QLFKCKVSLN STLNDVLHQS IQFFQLHTSS NDWSLIHLDK
      51  PVPLDLPWRL LNLPTGVNLE LSKSSNFPVA NKTNREDIPF NTIKIRFQIP
     101  GRDSVVKEMP SDQPIAPILR QMSGAAGDDF KIQVFSKIIE FKTIKDENLT
     151  LENLGIQEPS SVRLIFNNTS HSEGISANSA IHPKQTPPTM TNPETVASLP
     201  PHELHKPSVF LPSDEPLAVI KDQIEDEEDY ELTVEQAKKY QKMLSSKAGT
     251  LGGPILTKRL REQSANNLPK KNKAISECLL RVKFPDRSHI QIAFKPNEDM
     301  RTVYNVVSQF LIDENMPFTL NQSHPFKPLA KDDKKLLDDL EFGSKTMLLF
     351  ETNSNSNGPL IKAHLLEDAQ KITHETRTTP SVNTINKSNP QGPSDNATSI
     401  KKTLNRVPKW MKLSKK*

external links for Ubx4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Ubx4p

1)	Schuberth C, et al. (2004) Shp1 and Ubx2 are adaptors of Cdc48 involved in ubiquitin-dependent protein degradation. EMBO Rep 5(8):818-24
2)	Decottignies A, et al. (2004) Binding of Cdc48p to a ubiquitin-related UBX domain from novel yeast proteins involved in intracellular proteolysis and sporulation. Yeast 21(2):127-39
3)	Alberts SM, et al. (2009) Ubx4 modulates cdc48 activity and influences degradation of misfolded proteins of the endoplasmic reticulum. J Biol Chem 284(24):16082-9
4)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41