BUB2/YMR055C Protein Information

Standard Name	Bub2p
Systematic Name	Ymr055cp
Alias	Pac7p 1
ORF Classification	Verified
Description	Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage (2, 3, 4, 5, 6, 7, 8)
Name Description	Budding Uninhibited by Benzimidazole 9

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	306
Molecular Weight (Da)	35,027
Isoelectric Point (pI)	8.69

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Bub2p (InterPro)
Transmembrane Domains	There are 1 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 33 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000126B2F \| P26448 MIPS: YMR055C NCBI: 115149 \| 171135 \| 6323700 \| 817888 \| NP_013771.1 GenBank/EMBL/DDBJ: DAA09954.1 \| M64706 \| Z49703

Amino Acid Sequence (or in FASTA format)
1 MTSIEDLISN PPLLLHSSLS QLRYLILSEG LPISEDKQQQ RTRCYVWTVL 51 SQTSMEASTQ RYLALLKLGP PSTTIYQKIK NDTSRTFQTD PNFRNRVSED 101 ALIRCLSCFA WQTQQRRQKT RFGRIPVSTY VQGMNVLLAP LLYSCPSEPM 151 AYQLFTKLCY EMIPTYLTKN LNGAQNGAKL LDISLRIIDP KLSKFLSDNL 201 LTAEIYGMPS ILTLSSCNKP LDQVIKLWDF MFAYGFHMNI LFVVAFLVKM 251 RSKVFKSDSP VNLLRQFPDF DADEIIRLGV GFIAKIPAQI YDLLVDHLTD 301 PDIYIP*

Amino Acid Sequence (or in FASTA format)

       1  MTSIEDLISN PPLLLHSSLS QLRYLILSEG LPISEDKQQQ RTRCYVWTVL
      51  SQTSMEASTQ RYLALLKLGP PSTTIYQKIK NDTSRTFQTD PNFRNRVSED
     101  ALIRCLSCFA WQTQQRRQKT RFGRIPVSTY VQGMNVLLAP LLYSCPSEPM
     151  AYQLFTKLCY EMIPTYLTKN LNGAQNGAKL LDISLRIIDP KLSKFLSDNL
     201  LTAEIYGMPS ILTLSSCNKP LDQVIKLWDF MFAYGFHMNI LFVVAFLVKM
     251  RSKVFKSDSP VNLLRQFPDF DADEIIRLGV GFIAKIPAQI YDLLVDHLTD
     301  PDIYIP*

external links for Bub2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Bub2p

1)	Geiser JR, et al. (1997) Saccharomyces cerevisiae genes required in the absence of the CIN8-encoded spindle motor act in functionally diverse mitotic pathways. Mol Biol Cell 8(6):1035-50
2)	Fraschini R, et al. (1999) Budding yeast Bub2 is localized at spindle pole bodies and activates the mitotic checkpoint via a different pathway from Mad2. J Cell Biol 145(5):979-91
3)	Pereira G, et al. (2000) The Bub2p spindle checkpoint links nuclear migration with mitotic exit. Mol Cell 6(1):1-10
4)	Krishnan R, et al. (2000) Saccharomyces cerevisiae BUB2 prevents mitotic exit in response to both spindle and kinetochore damage. Genetics 156(2):489-500
5)	Hu F, et al. (2001) Regulation of the Bub2/Bfa1 GAP complex by Cdc5 and cell cycle checkpoints. Cell 107(5):655-65
6)	Pereira G, et al. (2001) Modes of spindle pole body inheritance and segregation of the Bfa1p-Bub2p checkpoint protein complex. EMBO J 20(22):6359-70
7)	Pereira G, et al. (2002) Regulation of the Bfa1p-Bub2p complex at spindle pole bodies by the cell cycle phosphatase Cdc14p. J Cell Biol 157(3):367-79
8)	Molk JN, et al. (2004) The differential roles of budding yeast Tem1p, Cdc15p, and Bub2p protein dynamics in mitotic exit. Mol Biol Cell 15(4):1519-32
9)	Hoyt MA, et al. (1991) S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function. Cell 66(3):507-17