CSM3/YMR048W Protein Information

Standard Name	Csm3p 1
Systematic Name	Ymr048wp
ORF Classification	Verified
Description	Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress (1, 2, 3, 4, 5)
Name Description	Chromosome Segregation in Meiosis 1

Experimental Data
Molecules/cell	358 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	317
Molecular Weight (Da)	36,354
Isoelectric Point (pI)	4.61

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Csm3p (InterPro)
Physical Interactions	There are 51 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013BA24 \| Q04659 MIPS: YMR048W NCBI: 2497131 \| 45270690 \| 6323692 \| 817881 \| NP_013763.1 GenBank/EMBL/DDBJ: DAA09947.1 \| AY558400 \| Z49703

Amino Acid Sequence (or in FASTA format)
1 MDQDFDSLLL GFNDSDSVQK DPTVPNGLDG SVVDPTIADP TAITARKRRP 51 QVKLTAEKLL SDKGLPYVLK NAHKRIRISS KKNSYDNLSN IIQFYQLWAH 101 ELFPKAKFKD FMKICQTVGK TDPVLREYRV SLFRDEMGMS FDVGTRETGQ 151 DLERQSPMVE EHVTSAEERP IVADSFAQDK RNVNNVDYDN DEDDDIYHLS 201 YRNRRGRVLD ERGNNETVLN NVVPPKEDLD ALLKTFRVQG PVGLEENEKK 251 LLLGWLDAHR KMEKGSMTEE DVQLIQSLEE WEMNDIEGQH THYDLLPGGD 301 EFGVDQDELD AMKEMGF*

Amino Acid Sequence (or in FASTA format)

       1  MDQDFDSLLL GFNDSDSVQK DPTVPNGLDG SVVDPTIADP TAITARKRRP
      51  QVKLTAEKLL SDKGLPYVLK NAHKRIRISS KKNSYDNLSN IIQFYQLWAH
     101  ELFPKAKFKD FMKICQTVGK TDPVLREYRV SLFRDEMGMS FDVGTRETGQ
     151  DLERQSPMVE EHVTSAEERP IVADSFAQDK RNVNNVDYDN DEDDDIYHLS
     201  YRNRRGRVLD ERGNNETVLN NVVPPKEDLD ALLKTFRVQG PVGLEENEKK
     251  LLLGWLDAHR KMEKGSMTEE DVQLIQSLEE WEMNDIEGQH THYDLLPGGD
     301  EFGVDQDELD AMKEMGF*

external links for Csm3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Csm3p

1)	Rabitsch KP, et al. (2001) A screen for genes required for meiosis and spore formation based on whole-genome expression. Curr Biol 11(13):1001-9
2)	Calzada A, et al. (2005) Molecular anatomy and regulation of a stable replisome at a paused eukaryotic DNA replication fork. Genes Dev 19(16):1905-19
3)	Mohanty BK, et al. (2006) The Tof1p-Csm3p protein complex counteracts the Rrm3p helicase to control replication termination of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 103(4):897-902
4)	Bando M, et al. (2009) Csm3, Tof1, and Mrc1 form a heterotrimeric mediator complex that associates with DNA replication forks. J Biol Chem 284(49):34355-65
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41