MSS1/YMR023C Protein Information

Standard Name	Mss1p
Systematic Name	Ymr023cp
Alias	Pet53p
ORF Classification	Verified
Description	Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 (1, 2, 3, 4)
Name Description	Mitochondrial Splicing System

Experimental Data
Molecules/cell	768 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	526
Molecular Weight (Da)	58,351
Isoelectric Point (pI)	7.73

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Mss1p (InterPro)
Physical Interactions	There are 3 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012F689 \| P32559 MIPS: YMR023C NCBI: 110610140 \| 1709142 \| 4006 \| 6323665 \| 798934 \| NP_013736.1 GenBank/EMBL/DDBJ: DAA09921.1 \| DQ834923 \| X69481 \| Z49211

Amino Acid Sequence (or in FASTA format)
1 MNSASFLQSR LISRSFLVRR SLKRYSGLAK PYTFQQPTIY ALSTPANQTS 51 AIAIIRISGT HAKYIYNRLV DSSTVPPIRK AILRNIYSPS SCSVKPHDQK 101 ESKILLDTSL LLYFQAPYSF TGEDVLELHV HGGKAVVNSI LKAIGSLHDR 151 SSGKDIRFAL PGDFSRRAFQ NGKFDLTQLE GIKDLIDSET ESQRRSALSS 201 FNGDNKILFE NWRETIIENM AQLTAIIDFA DDNSQEIQNT DEIFHNVEKN 251 IICLRDQIVT FMQKVEKSTI LQNGIKLVLL GAPNVGKSSL VNSLTNDDIS 301 IVSDIPGTTR DSIDAMINVN GYKVIICDTA GIREKSSDKI EMLGIDRAKK 351 KSVQSDLCLF IVDPTDLSKL LPEDILAHLS SKTFGNKRII IVVNKSDLVS 401 DDEMTKVLNK LQTRLGSKYP ILSVSCKTKE GIESLISTLT SNFESLSQSS 451 ADASPVIVSK RVSEILKNDV LYGLEEFFKS KDFHNDIVLA TENLRYASDG 501 IAKITGQAIG IEEILDSVFS KFCIGK*

Amino Acid Sequence (or in FASTA format)

       1  MNSASFLQSR LISRSFLVRR SLKRYSGLAK PYTFQQPTIY ALSTPANQTS
      51  AIAIIRISGT HAKYIYNRLV DSSTVPPIRK AILRNIYSPS SCSVKPHDQK
     101  ESKILLDTSL LLYFQAPYSF TGEDVLELHV HGGKAVVNSI LKAIGSLHDR
     151  SSGKDIRFAL PGDFSRRAFQ NGKFDLTQLE GIKDLIDSET ESQRRSALSS
     201  FNGDNKILFE NWRETIIENM AQLTAIIDFA DDNSQEIQNT DEIFHNVEKN
     251  IICLRDQIVT FMQKVEKSTI LQNGIKLVLL GAPNVGKSSL VNSLTNDDIS
     301  IVSDIPGTTR DSIDAMINVN GYKVIICDTA GIREKSSDKI EMLGIDRAKK
     351  KSVQSDLCLF IVDPTDLSKL LPEDILAHLS SKTFGNKRII IVVNKSDLVS
     401  DDEMTKVLNK LQTRLGSKYP ILSVSCKTKE GIESLISTLT SNFESLSQSS
     451  ADASPVIVSK RVSEILKNDV LYGLEEFFKS KDFHNDIVLA TENLRYASDG
     501  IAKITGQAIG IEEILDSVFS KFCIGK*

external links for Mss1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Mss1p

1)	Decoster E, et al. (1993) MSS1, a nuclear-encoded mitochondrial GTPase involved in the expression of COX1 subunit of cytochrome c oxidase. J Mol Biol 232(1):79-88
2)	Colby G, et al. (1998) MTO1 codes for a mitochondrial protein required for respiration in paromomycin-resistant mutants of Saccharomyces cerevisiae. J Biol Chem 273(43):27945-52
3)	Li X and Guan MX (2002) A human mitochondrial GTP binding protein related to tRNA modification may modulate phenotypic expression of the deafness-associated mitochondrial 12S rRNA mutation. Mol Cell Biol 22(21):7701-11
4)	Umeda N, et al. (2005) Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs. Implications for the molecular pathogenesis of human mitochondrial diseases. J Biol Chem 280(2):1613-24
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41