PHO84/YML123C Protein Information Help

Standard Name Pho84p
Systematic Name Yml123cp
ORF Classification Verified
Description High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity (1, 2, 3, 4, 5) Also known as: phoT 6
Name Description PHOsphate metabolism
Experimental Data
Molecules/cell 335000 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 587
Molecular Weight (Da) 64,381
Isoelectric Point (pI) 6.41

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Pho84p (InterPro)
Transmembrane Domains There are 9 total predicted transmembrane domains (TMHMM)
Physical Interactions There are 39 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00001318AC | P25297
MIPS: YML123C
NCBI: 1346710 | 218455 | 6323512 | 805019 | NP_013583.1 | NM_001182486.1
GenBank/EMBL/DDBJ: DAA09776.1 | D90346 | Z49218
External Classifications TC: 2.A.1.9 [The Phosphate:H+ Symporter (PHS) Family]
Amino Acid Sequence (or in FASTA format)
 
       1  MSSVNKDTIH VAERSLHKEH LTEGGNMAFH NHLNDFAHIE DPLERRRLAL
      51  ESIDDEGFGW QQVKTISIAG VGFLTDSYDI FAINLGITMM SYVYWHGSMP
     101  GPSQTLLKVS TSVGTVIGQF GFGTLADIVG RKRIYGMELI IMIVCTILQT
     151  TVAHSPAINF VAVLTFYRIV MGIGIGGDYP LSSIITSEFA TTKWRGAIMG
     201  AVFANQAWGQ ISGGIIALIL VAAYKGELEY ANSGAECDAR CQKACDQMWR
     251  ILIGLGTVLG LACLYFRLTI PESPRYQLDV NAKLELAAAA QEQDGEKKIH
     301  DTSDEDMAIN GLERASTAVE SLDNHPPKAS FKDFCRHFGQ WKYGKILLGT
     351  AGSWFTLDVA FYGLSLNSAV ILQTIGYAGS KNVYKKLYDT AVGNLILICA
     401  GSLPGYWVSV FTVDIIGRKP IQLAGFIILT ALFCVIGFAY HKLGDHGLLA
     451  LYVICQFFQN FGPNTTTFIV PGECFPTRYR STAHGISAAS GKVGAIIAQT
     501  ALGTLIDHNC ARDGKPTNCW LPHVMEIFAL FMLLGIFTTL LIPETKRKTL
     551  EEINELYHDE IDPATLNFRN KNNDIESSSP SQLQHEA*

external links for Pho84p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome TCDB
YGOB DIP YeastRC Structure Prediction (Seattle)
YOGY GeneMANIA


YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Pho84p
1) Bun-ya M, et al.  (1996) Two new genes, PHO86 and PHO87, involved in inorganic phosphate uptake in Saccharomyces cerevisiae. Curr Genet 29(4):344-51
2) Nishimura K, et al.  (1999) Transcription of some PHO genes in Saccharomyces cerevisiae is regulated by spt7p. Yeast 15(16):1711-7
3) Bun-Ya M, et al.  (1991) The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter. Mol Cell Biol 11(6):3229-38
4) Jensen LT, et al.  (2003) The Saccharomyces cerevisiae high affinity phosphate transporter encoded by PHO84 also functions in manganese homeostasis. J Biol Chem 278(43):42036-40
5) Ofiteru AM, et al.  (2012) Overexpression of the PHO84 gene causes heavy metal accumulation and induces Ire1p-dependent unfolded protein response in Saccharomyces cerevisiae cells. Appl Microbiol Biotechnol 94(2):425-35
6) Ueda Y, et al.  (1975) Isolation and characterization of recessive, constitutive mutations for repressible acid phosphatase synthesis in Saccharomyces cerevisiae. J Bacteriol 122(3):911-22
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41