CAC2/YML102W Protein Information

Standard Name	Cac2p 1
Systematic Name	Yml102wp
ORF Classification	Verified
Description	Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription (1, 10, 2, 3, 4, 5, 6, 7, 8, 9)
Name Description	Chromatin Assembly Complex 1

Experimental Data
Molecules/cell	2130 11

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	468
Molecular Weight (Da)	51,252
Isoelectric Point (pI)	6.3

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Cac2p (InterPro)
Physical Interactions	There are 43 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000126D77 \| Q04199 MIPS: YML102W NCBI: 2494909 \| 530342 \| 6323534 \| NP_013605.1 GenBank/EMBL/DDBJ: DAA09796.1 \| X80835

Amino Acid Sequence (or in FASTA format)
1 MEASHLQIYW HDSQPVYSLT FQKNSANDKL FTAGGDNKVR IWKLNRDENG 51 QNGGVRKIES LDFLGSLTHH EQAINVIRFN SKGDVLASAG DDGQVLLWKQ 101 EDPNTQQESV VRPFGMDAET SEADENKEKW VVWKRLRGGS GATAAAEIYD 151 LAWSPDNRNI VVACMDNSIR LFDVGAGMLV CGQSDHGHYV QGVAWDPLNQ 201 FILSQSADRS LHVYGVILSS AGVVTGLKLR SKIAKAELPC PGDVLRTNYL 251 FHNETLPSFF RRCSISPCGG LVVIPSGVYK VAGDEVANCV YVYTRSGILN 301 SAGGVKNRPA IRIPSLKKPA LMAAFSPVFY ETCQKSVLKL PYKLVFAIAT 351 TNEVLVYDTD VLEPLCVVGN IHYSPITDLA WSEDGSTLLI SSTDGFCSYV 401 SIDTETQFGS RIEPPAMHAE PLDTDESAVA AKNQREAGGI VNMLPVKKIP 451 CNSSDSKKRR IHPTPVDL*

Amino Acid Sequence (or in FASTA format)

       1  MEASHLQIYW HDSQPVYSLT FQKNSANDKL FTAGGDNKVR IWKLNRDENG
      51  QNGGVRKIES LDFLGSLTHH EQAINVIRFN SKGDVLASAG DDGQVLLWKQ
     101  EDPNTQQESV VRPFGMDAET SEADENKEKW VVWKRLRGGS GATAAAEIYD
     151  LAWSPDNRNI VVACMDNSIR LFDVGAGMLV CGQSDHGHYV QGVAWDPLNQ
     201  FILSQSADRS LHVYGVILSS AGVVTGLKLR SKIAKAELPC PGDVLRTNYL
     251  FHNETLPSFF RRCSISPCGG LVVIPSGVYK VAGDEVANCV YVYTRSGILN
     301  SAGGVKNRPA IRIPSLKKPA LMAAFSPVFY ETCQKSVLKL PYKLVFAIAT
     351  TNEVLVYDTD VLEPLCVVGN IHYSPITDLA WSEDGSTLLI SSTDGFCSYV
     401  SIDTETQFGS RIEPPAMHAE PLDTDESAVA AKNQREAGGI VNMLPVKKIP
     451  CNSSDSKKRR IHPTPVDL*

external links for Cac2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Cac2p

1)	Kaufman PD, et al. (1997) Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I. Genes Dev 11(3):345-57
2)	Enomoto S and Berman J (1998) Chromatin assembly factor I contributes to the maintenance, but not the re-establishment, of silencing at the yeast silent mating loci. Genes Dev 12(2):219-32
3)	Game JC and Kaufman PD (1999) Role of Saccharomyces cerevisiae chromatin assembly factor-I in repair of ultraviolet radiation damage in vivo. Genetics 151(2):485-97
4)	Smith JS, et al. (1999) A genetic screen for ribosomal DNA silencing defects identifies multiple DNA replication and chromatin-modulating factors. Mol Cell Biol 19(4):3184-97
5)	Sharp JA, et al. (2002) Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae. Genes Dev 16(1):85-100
6)	Krude T (2002) Chromatin assembly: the kinetochore connection. Curr Biol 12(7):R256-8
7)	Zabaronick SR and Tyler JK (2005) The histone chaperone anti-silencing function 1 is a global regulator of transcription independent of passage through S phase. Mol Cell Biol 25(2):652-60
8)	Zhou H, et al. (2006) Chromatin assembly factor 1 interacts with histone H3 methylated at lysine 79 in the processes of epigenetic silencing and DNA repair. Biochemistry 45(9):2852-61
9)	Kim JA and Haber JE (2009) Chromatin assembly factors Asf1 and CAF-1 have overlapping roles in deactivating the DNA damage checkpoint when DNA repair is complete. Proc Natl Acad Sci U S A 106(4):1151-6
10)	Kim HJ, et al. (2009) Potential role of the histone chaperone, CAF-1, in transcription. BMB Rep 42(4):227-31
11)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41