TDA9/YML081W Protein Information Help

Standard Name Tda9p 1
Systematic Name Yml081wp
Alias Aaf1p 2
ORF Classification Verified
Description Transcription factor that regulates acetate production; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7)
Name Description Topoisomerase I Damage Affected 1
Experimental Data
Molecules/cell 672 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,251
Molecular Weight (Da) 141,464
Isoelectric Point (pI) 7.72

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Tda9p (InterPro)
Transmembrane Domains There are 1 total predicted transmembrane domains (TMHMM)
Physical Interactions There are 4 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013B9D1 | Q04545
MIPS: YML081W
NCBI: 2501692 | 575722 | 587530 | 6323559 | NP_013630.1 | NM_001182440.1
GenBank/EMBL/DDBJ: CAA86497.1 | CAA86657.1 | DAA09816.1 | Z46373 | Z46660
Amino Acid Sequence (or in FASTA format)
       1  MSSEEFKGLP IKRDISSTIY ADRPPALSAP PCVGATGNDK IQVLPIPKKS
      51  RTIKTDKPRP FLCHICTRGF VRQEHLKRHQ RAHTNEKPFL CVFCGRCFAR
     101  RDLVLRHQHK LHSALVSKES INSKDKTEID AINDKNIIQI QGNKQTILPT
     151  PSNPLAKTAV QLKKAAKEKK NGKQGKLDLS PSYGANNHST DVSPSVGNSS
     201  TPAVIEETDS SSHFPLPDTN IPTKSKRHAS FSASSAFTYS SDNFQKLHQQ
     251  AKSDFDELQE SIPHQVGFST PQLTAQQLIE NAIESGVVDL ETLDLPPFLS
     301  LDGLPPASSS AAVAASEQID ICPSSATDTI SGANSTPNQA ATAPPFQLPI
     351  ARESSSLFLA NTPYLSDFLT MGSSYGGSGG FAKSITADPS LDYFNYKNHS
     401  HPDSRHNNSS SGINYSNNKN NNESIEKSQN NSNVINETID HTDIHAHHAD
     451  AHDDSFIESE EWLSKFIMDS QIDNDLKLNI NHFNDIGFNN LHPQNPTTHS
     501  EPRNMHNENR DMHRSASKFQ SVSENISPRE QMSLFKTKQN KAISKFLSDE
     551  KIPSTASPSS SASPVQFDKK NVDINEFLLD ESVSNLFTTR QIDLFKKNVN
     601  LYSPLFQNQK DAVSSTSLTP SLTTQTATTQ SGPGWTDSSQ KLTFFTEQLR
     651  NLIIKENNLK SNLFPTVDEL NHYVDLYQVE FHKYFPFIHL YSIIPSSENY
     701  PLVISISMIG ALYGFHSTHA LLLSKIARTR VRMFLENTRS NHDKTPIWLM
     751  QSLVLLTFTS IFSNDMNAFR TVNTQIMILV QLIKISKLNY PLENFIKPPI
     801  ESDHVLEYQD NPAVLNQFKA QYNTREQINR NFKYFILAQS RIRICHIVLL
     851  ISNLFKSLVD FDCCFHSIDL KCGVPCYNEV LFFCENSRTW NENLTRFNIV
     901  LDSKFSLIEV SNGESNYEKC LMYLSNGNPY LYKNAKISFK TLLSLLISIH
     951  EKINIERDAL KDSYESDFHA KNVQWRMHSR PLVATMLKHW ELLYIKNGGI
    1001  LALSDENLPI INTNPSFRLI IPLYFFAKLR KCLDIAPTLR CIWNQDWNSM
    1051  NSSLEKVCYE RESLREATEY AVSVITFWID TVSVMKGKST QTPIFTITCI
    1101  FVSILVIAGY MRRLEDFAQN KNSDCMIGSL KSTDRILWLK AFKTLKRIES
    1151  HLSEREYKLQ TFAEFLRVPD NGSLDIESLD SSLIENTLNS HDVTNQALDI
    1201  ITRTRLSSRT LYCGARILGD TPVWPVSLLF AHALQSRAIY NINHRKSVNS
    1251  V*                                                    

external links for Tda9p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) CYC2008 (complexes) MIPS YeastGFP
YGOB Complexome Pfam domains YeastRC Public Image Repository
YOGY GeneMANIA YeastRC Structure Prediction (Seattle)

IMP

References cited on this page View Complete Literature Guide for Tda9p
1) Reid RJ, et al.  (2011) Selective ploidy ablation, a high-throughput plasmid transfer protocol, identifies new genes affecting topoisomerase I-induced DNA damage. Genome Res 21(3):477-86
2) Walkey CJ, et al.  (2012) The Fermentation Stress Response Protein Aaf1p/Yml081Wp Regulates Acetate Production in Saccharomyces cerevisiae. PLoS One 7(12):e51551
3) Giaever G, et al.  (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91
4) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87
7) Yang Y, et al.  (2010) Identifying cooperative transcription factors by combining ChIP-chip data and knockout data. Cell Res 20(11):1276-8
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41