TEM1/YML064C Protein Information

Standard Name	Tem1p
Systematic Name	Yml064cp
ORF Classification	Verified
Description	GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis (1, 2, 3)
Name Description	TErmination of M phase 1

Experimental Data
Molecules/cell	573 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	245
Molecular Weight (Da)	27,296
Isoelectric Point (pI)	10.22

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Tem1p (InterPro)
Physical Interactions	There are 136 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000136BAE \| P38987 MIPS: YML064C NCBI: 45269852 \| 537606 \| 558411 \| 6323576 \| 730928 \| NP_013647.1 GenBank/EMBL/DDBJ: DAA09833.1 \| AY557980 \| D38172 \| Z38114

Amino Acid Sequence (or in FASTA format)
1 MATPSTGANN SIPAVRNQVE VQVGLVGDAQ VGKTSLMVKY VQNIYDKEYT 51 QTLGVNFLKR KVSIRSTDII FSIMDLGGQR EFINMLPIAT VGSSVIIFLF 101 DLTRPETLSS IKEWYRQAYG LNDSAIPILV GTKYDLLIDL DPEYQEQISR 151 TSMKYAQVMN APLIFCSTAK SINIQKIFKI ALAKIFNLTL TIPEINEIGD 201 PLLIYKHLGG QQHRHHNKSQ DRKSHNIRKP SSSPSSKAPS PGVNT*

Amino Acid Sequence (or in FASTA format)

       1  MATPSTGANN SIPAVRNQVE VQVGLVGDAQ VGKTSLMVKY VQNIYDKEYT
      51  QTLGVNFLKR KVSIRSTDII FSIMDLGGQR EFINMLPIAT VGSSVIIFLF
     101  DLTRPETLSS IKEWYRQAYG LNDSAIPILV GTKYDLLIDL DPEYQEQISR
     151  TSMKYAQVMN APLIFCSTAK SINIQKIFKI ALAKIFNLTL TIPEINEIGD
     201  PLLIYKHLGG QQHRHHNKSQ DRKSHNIRKP SSSPSSKAPS PGVNT*

external links for Tem1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Tem1p

1)	Shirayama M, et al. (1994) The yeast TEM1 gene, which encodes a GTP-binding protein, is involved in termination of M phase. Mol Cell Biol 14(11):7476-82
2)	Shou W, et al. (1999) Exit from mitosis is triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex. Cell 97(2):233-44
3)	Lippincott J, et al. (2001) The Tem1 small GTPase controls actomyosin and septin dynamics during cytokinesis. J Cell Sci 114(Pt 7):1379-86
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41