PIF1/YML061C Protein Information Help

Standard Name Pif1p 1, 2
Systematic Name Yml061cp
Alias Tst1p
ORF Classification Verified
Description DNA helicase, potent G-quadruplex DNA binder and unwinder; promotes DNA synthesis during break-induced replication (BIR); important for crossover recombination; nuclear form acts as a catalytic inhibitor of telomerase; mitochondrial form is involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells (1, 3, 4, 5, 6, 7, 8, 9)
Name Description Petite Integration Frequency 2
Experimental Data
Molecules/cell 259 10
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 859
Molecular Weight (Da) 97,681
Isoelectric Point (pI) 9.72

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Pif1p (InterPro)
Signal Peptide(s) There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions There are 13 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00001683BA | P07271
MIPS: YML061C
NCBI: 130196 | 193806354 | 4178 | 558414 | 577135 | 6323579 | NP_013650.1 | NM_001182420.1
GenBank/EMBL/DDBJ: CAA28953.1 | CAA86260.1 | CAA86714.1 | DAA09836.1 | X05342 | Z38114 | Z46729
External Classifications EC: 3.6.4.12 [DNA helicase]
Amino Acid Sequence (or in FASTA format)
       1  MPKWIRSTLN HIIPRRPFIC SFNSFLLLKN VSHAKLSFSM SSRGFRSNNF
      51  IQAQLKHPSI LSKEDLDLLS DSDDWEEPDC IQLETEKQEK KIITDIHKED
     101  PVDKKPMRDK NVMNFINKDS PLSWNDMFKP SIIQPPQLIS ENSFDQSSQK
     151  KSRSTGFKNP LRPALKKESS FDELQNNSIS QERSLEMINE NEKKKMQFGE
     201  KIAVLTQRPS FTELQNDQDD SNLNPHNGVK VKIPICLSKE QESIIKLAEN
     251  GHNIFYTGSA GTGKSILLRE MIKVLKGIYG RENVAVTAST GLAACNIGGI
     301  TIHSFAGIGL GKGDADKLYK KVRRSRKHLR RWENIGALVV DEISMLDAEL
     351  LDKLDFIARK IRKNHQPFGG IQLIFCGDFF QLPPVSKDPN RPTKFAFESK
     401  AWKEGVKMTI MLQKVFRQRG DVKFIDMLNR MRLGNIDDET EREFKKLSRP
     451  LPDDEIIPAE LYSTRMEVER ANNSRLSKLP GQVHIFNAID GGALEDEELK
     501  ERLLQNFLAP KELHLKVGAQ VMMVKNLDAT LVNGSLGKVI EFMDPETYFC
     551  YEALTNDPSM PPEKLETWAE NPSKLKAAME REQSDGEESA VASRKSSVKE
     601  GFAKSDIGEP VSPLDSSVFD FMKRVKTDDE VVLENIKRKE QLMQTIHQNS
     651  AGKRRLPLVR FKASDMSTRM VLVEPEDWAI EDENEKPLVS RVQLPLMLAW
     701  SLSIHKSQGQ TLPKVKVDLR RVFEKGQAYV ALSRAVSREG LQVLNFDRTR
     751  IKAHQKVIDF YLTLSSAESA YKQLEADEQV KKRKLDYAPG PKYKAKSKSK
     801  SNSPAPISAT TQSNNGIAAM LQRHSRKRFQ LKKESNSNQV HSLVSDEPRG
     851  QDTEDHILE*                                            

external links for Pif1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily LoQate
Ashbya (AGD) BOND GPMdb (Mass Spec.) Organelle DB
Candida (CGD) CYC2008 (complexes) MIPS YPL+
YGOB Complexome Pfam domains YeastGFP
YOGY DIP YeastRC Structure Prediction (Seattle) YeastRC Public Image Repository

GeneMANIA


IMP

References cited on this page View Complete Literature Guide for Pif1p
1) Foury F and Lahaye A  (1987) Cloning and sequencing of the PIF gene involved in repair and recombination of yeast mitochondrial DNA. EMBO J 6(5):1441-9
2) Foury F and Kolodynski J  (1983) pif mutation blocks recombination between mitochondrial rho+ and rho- genomes having tandemly arrayed repeat units in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 80(17):5345-9
3) Ivessa AS, et al.  (2000) The Saccharomyces Pif1p DNA helicase and the highly related Rrm3p have opposite effects on replication fork progression in ribosomal DNA. Cell 100(4):479-89
4) Zhou J, et al.  (2000) Pif1p helicase, a catalytic inhibitor of telomerase in yeast. Science 289(5480):771-4
5) Vega LR, et al.  (2007) Sensitivity of yeast strains with long g-tails to levels of telomere-bound telomerase. PLoS Genet 3(6):e105
6) Guirola M, et al.  (2010) Lack of DNA helicase Pif1 disrupts zinc and iron homoeostasis in yeast. Biochem J 432(3):595-605
7) Crider DG, et al.  (2012) Rad53 is essential for a mitochondrial DNA inheritance checkpoint regulating G1 to S progression. J Cell Biol 198(5):793-8
8) Paeschke K, et al.  (2013) Pif1 family helicases suppress genome instability at G-quadruplex motifs. Nature 497(7450):458-62
9) Wilson MA, et al.  (2013) Pif1 helicase and Pold promote recombination-coupled DNA synthesis via bubble migration. Nature 502(7471):393-6
10) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41