PIF1/YML061C Protein Information

Standard Name	Pif1p 1, 2
Systematic Name	Yml061cp
Alias	Tst1p
ORF Classification	Verified
Description	DNA helicase, potent G-quadruplex DNA binder and unwinder; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells (1, 3, 4, 5, 6, 7, 8)
Name Description	Petite Integration Frequency 2

Experimental Data
Molecules/cell	259 9

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	859
Molecular Weight (Da)	97,681
Isoelectric Point (pI)	9.72

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pif1p (InterPro)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 12 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001683BA \| P07271 MIPS: YML061C NCBI: 130196 \| 193806354 \| 4178 \| 558414 \| 577135 \| 6323579 \| NP_013650.1 GenBank/EMBL/DDBJ: DAA09836.1 \| X05342 \| Z38114 \| Z46729
External Classifications	EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides]

Amino Acid Sequence (or in FASTA format)
1 MPKWIRSTLN HIIPRRPFIC SFNSFLLLKN VSHAKLSFSM SSRGFRSNNF 51 IQAQLKHPSI LSKEDLDLLS DSDDWEEPDC IQLETEKQEK KIITDIHKED 101 PVDKKPMRDK NVMNFINKDS PLSWNDMFKP SIIQPPQLIS ENSFDQSSQK 151 KSRSTGFKNP LRPALKKESS FDELQNNSIS QERSLEMINE NEKKKMQFGE 201 KIAVLTQRPS FTELQNDQDD SNLNPHNGVK VKIPICLSKE QESIIKLAEN 251 GHNIFYTGSA GTGKSILLRE MIKVLKGIYG RENVAVTAST GLAACNIGGI 301 TIHSFAGIGL GKGDADKLYK KVRRSRKHLR RWENIGALVV DEISMLDAEL 351 LDKLDFIARK IRKNHQPFGG IQLIFCGDFF QLPPVSKDPN RPTKFAFESK 401 AWKEGVKMTI MLQKVFRQRG DVKFIDMLNR MRLGNIDDET EREFKKLSRP 451 LPDDEIIPAE LYSTRMEVER ANNSRLSKLP GQVHIFNAID GGALEDEELK 501 ERLLQNFLAP KELHLKVGAQ VMMVKNLDAT LVNGSLGKVI EFMDPETYFC 551 YEALTNDPSM PPEKLETWAE NPSKLKAAME REQSDGEESA VASRKSSVKE 601 GFAKSDIGEP VSPLDSSVFD FMKRVKTDDE VVLENIKRKE QLMQTIHQNS 651 AGKRRLPLVR FKASDMSTRM VLVEPEDWAI EDENEKPLVS RVQLPLMLAW 701 SLSIHKSQGQ TLPKVKVDLR RVFEKGQAYV ALSRAVSREG LQVLNFDRTR 751 IKAHQKVIDF YLTLSSAESA YKQLEADEQV KKRKLDYAPG PKYKAKSKSK 801 SNSPAPISAT TQSNNGIAAM LQRHSRKRFQ LKKESNSNQV HSLVSDEPRG 851 QDTEDHILE*

Amino Acid Sequence (or in FASTA format)

       1  MPKWIRSTLN HIIPRRPFIC SFNSFLLLKN VSHAKLSFSM SSRGFRSNNF
      51  IQAQLKHPSI LSKEDLDLLS DSDDWEEPDC IQLETEKQEK KIITDIHKED
     101  PVDKKPMRDK NVMNFINKDS PLSWNDMFKP SIIQPPQLIS ENSFDQSSQK
     151  KSRSTGFKNP LRPALKKESS FDELQNNSIS QERSLEMINE NEKKKMQFGE
     201  KIAVLTQRPS FTELQNDQDD SNLNPHNGVK VKIPICLSKE QESIIKLAEN
     251  GHNIFYTGSA GTGKSILLRE MIKVLKGIYG RENVAVTAST GLAACNIGGI
     301  TIHSFAGIGL GKGDADKLYK KVRRSRKHLR RWENIGALVV DEISMLDAEL
     351  LDKLDFIARK IRKNHQPFGG IQLIFCGDFF QLPPVSKDPN RPTKFAFESK
     401  AWKEGVKMTI MLQKVFRQRG DVKFIDMLNR MRLGNIDDET EREFKKLSRP
     451  LPDDEIIPAE LYSTRMEVER ANNSRLSKLP GQVHIFNAID GGALEDEELK
     501  ERLLQNFLAP KELHLKVGAQ VMMVKNLDAT LVNGSLGKVI EFMDPETYFC
     551  YEALTNDPSM PPEKLETWAE NPSKLKAAME REQSDGEESA VASRKSSVKE
     601  GFAKSDIGEP VSPLDSSVFD FMKRVKTDDE VVLENIKRKE QLMQTIHQNS
     651  AGKRRLPLVR FKASDMSTRM VLVEPEDWAI EDENEKPLVS RVQLPLMLAW
     701  SLSIHKSQGQ TLPKVKVDLR RVFEKGQAYV ALSRAVSREG LQVLNFDRTR
     751  IKAHQKVIDF YLTLSSAESA YKQLEADEQV KKRKLDYAPG PKYKAKSKSK
     801  SNSPAPISAT TQSNNGIAAM LQRHSRKRFQ LKKESNSNQV HSLVSDEPRG
     851  QDTEDHILE*

external links for Pif1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Pif1p

1)	Foury F and Lahaye A (1987) Cloning and sequencing of the PIF gene involved in repair and recombination of yeast mitochondrial DNA. EMBO J 6(5):1441-9
2)	Foury F and Kolodynski J (1983) pif mutation blocks recombination between mitochondrial rho+ and rho- genomes having tandemly arrayed repeat units in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 80(17):5345-9
3)	Ivessa AS, et al. (2000) The Saccharomyces Pif1p DNA helicase and the highly related Rrm3p have opposite effects on replication fork progression in ribosomal DNA. Cell 100(4):479-89
4)	Zhou J, et al. (2000) Pif1p helicase, a catalytic inhibitor of telomerase in yeast. Science 289(5480):771-4
5)	Vega LR, et al. (2007) Sensitivity of yeast strains with long g-tails to levels of telomere-bound telomerase. PLoS Genet 3(6):e105
6)	Guirola M, et al. (2010) Lack of DNA helicase Pif1 disrupts zinc and iron homoeostasis in yeast. Biochem J 432(3):595-605
7)	Crider DG, et al. (2012) Rad53 is essential for a mitochondrial DNA inheritance checkpoint regulating G1 to S progression. J Cell Biol 198(5):793-8
8)	Paeschke K, et al. (2013) Pif1 family helicases suppress genome instability at G-quadruplex motifs. Nature ()
9)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41