RAD52/YML032C Protein Information

Standard Name	Rad52p
Systematic Name	Yml032cp
ORF Classification	Verified
Description	Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis (1, 2, 3, 4)
Name Description	RADiation sensitive

Experimental Data
Molecules/cell	1080 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	471
Molecular Weight (Da)	52,403
Isoelectric Point (pI)	8.56

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rad52p (InterPro)
Physical Interactions	There are 102 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00001683C2 \| P06778 MIPS: YML032C NCBI: 109940131 \| 131814 \| 172341 \| 27808713 \| 51013739 \| 575687 \| NP_013680.2 GenBank/EMBL/DDBJ: DAA09866.1 \| AY693144 \| M10249 \| Z46659

Amino Acid Sequence (or in FASTA format)
1 MNEIMDMDEK KPVFGNHSED IQTKLDKKLG PEYISKRVGF GTSRIAYIEG 51 WRVINLANQI FGYNGWSTEV KSVVIDFLDE RQGKFSIGCT AIVRVTLTSG 101 TYREDIGYGT VENERRKPAA FERAKKSAVT DALKRSLRGF GNALGNCLYD 151 KDFLAKIDKV KFDPPDFDEN NLFRPTDEIS ESSRTNTLHE NQEQQQYPNK 201 RRQLTKVTNT NPDSTKNLVK IENTVSRGTP MMAAPAEANS KNSSNKDTDL 251 KSLDASKQDQ DDLLDDSLMF SDDFQDDDLI NMGNTNSNVL TTEKDPVVAK 301 QSPTASSNPE AEQITFVTAK AATSVQNERY IGEESIFDPK YQAQSIRHTV 351 DQTTSKHIPA SVLKDKTMTT ARDSVYEKFA PKGKQLSMKN NDKELGPHML 401 EGAGNQVPRE TTPIKTNATA FPPAAAPRFA PPSKVVHPNG NGAVPAVPQQ 451 RSTRREVGRP KINPLHARKP T*

Amino Acid Sequence (or in FASTA format)

       1  MNEIMDMDEK KPVFGNHSED IQTKLDKKLG PEYISKRVGF GTSRIAYIEG
      51  WRVINLANQI FGYNGWSTEV KSVVIDFLDE RQGKFSIGCT AIVRVTLTSG
     101  TYREDIGYGT VENERRKPAA FERAKKSAVT DALKRSLRGF GNALGNCLYD
     151  KDFLAKIDKV KFDPPDFDEN NLFRPTDEIS ESSRTNTLHE NQEQQQYPNK
     201  RRQLTKVTNT NPDSTKNLVK IENTVSRGTP MMAAPAEANS KNSSNKDTDL
     251  KSLDASKQDQ DDLLDDSLMF SDDFQDDDLI NMGNTNSNVL TTEKDPVVAK
     301  QSPTASSNPE AEQITFVTAK AATSVQNERY IGEESIFDPK YQAQSIRHTV
     351  DQTTSKHIPA SVLKDKTMTT ARDSVYEKFA PKGKQLSMKN NDKELGPHML
     401  EGAGNQVPRE TTPIKTNATA FPPAAAPRFA PPSKVVHPNG NGAVPAVPQQ
     451  RSTRREVGRP KINPLHARKP T*

external links for Rad52p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Rad52p

1)	Adzuma K, et al. (1984) Primary structure of the RAD52 gene in Saccharomyces cerevisiae. Mol Cell Biol 4(12):2735-44
2)	Shinohara A and Ogawa T (1998) Stimulation by Rad52 of yeast Rad51-mediated recombination. Nature 391(6665):404-7
3)	Mortensen UH, et al. (1996) DNA strand annealing is promoted by the yeast Rad52 protein. Proc Natl Acad Sci U S A 93(20):10729-34
4)	Symington LS (2002) Role of RAD52 epistasis group genes in homologous recombination and double-strand break repair. Microbiol Mol Biol Rev 66(4):630-70, table of contents
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41