RCF1/YML030W Protein Information Help

Standard Name Rcf1p 1
Systematic Name Yml030wp
Alias Aim31p 2
ORF Classification Verified
Description Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast (1, 3, 4, 5)
Name Description Respiratory superComplex Factor 1
Experimental Data
Molecules/cell 1210 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 159
Molecular Weight (Da) 18,477
Isoelectric Point (pI) 9.83

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Rcf1p (InterPro)
Transmembrane Domains There are 2 total predicted transmembrane domains (TMHMM)
Physical Interactions There are 56 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052DF1 | Q03713
MIPS: YML030W
NCBI: 2497100 | 45270358 | 575689 | 6323611 | NP_013682.1
GenBank/EMBL/DDBJ: DAA09868.1 | AY558234 | Z46659
Amino Acid Sequence (or in FASTA format)
 
       1  MSRMPSSFDV TERDLDDMTF GERIIYHCKK QPLVPIGCLL TTGAVILAAQ
      51  NVRLGNKWKA QYYFRWRVGL QAATLVALVA GSFIYGTSGK ELKAKEEQLK
     101  EKAKMREKLW IQELERREEE TEARRKRAEL ARMKTLENEE EIKNLEKELS
     151  DLENKLGKK*

external links for Rcf1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome

YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Rcf1p
1) Strogolova V, et al.  (2012) Rcf1 and Rcf2, members of the hypoxia-induced gene 1 protein family, are critical components of the mitochondrial cytochrome bc1-cytochrome c oxidase supercomplex. Mol Cell Biol 32(8):1363-73
2) Hess DC, et al.  (2009) Computationally driven, quantitative experiments discover genes required for mitochondrial biogenesis. PLoS Genet 5(3):e1000407
3) Helbig AO, et al.  (2009) A three-way proteomics strategy allows differential analysis of yeast mitochondrial membrane protein complexes under anaerobic and aerobic conditions. Proteomics 9(20):4787-98
4) Vukotic M, et al.  (2012) Rcf1 mediates cytochrome oxidase assembly and respirasome formation, revealing heterogeneity of the enzyme complex. Cell Metab 15(3):336-47
5) Chen YC, et al.  (2012) Identification of a protein mediating respiratory supercomplex stability. Cell Metab 15(3):348-60
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41